Abstract: A novel method for the labeling of oligonucleotides which results in the economical synthesis of 5? labeled molecules. A set of suitably protected and carefully selected set of amino linkers, a modified deprotination/cleavage protocol and standard coupling methodologies to are used to allow for the convergent synthesis of any number of labeled oligonucleotides.

Abstract: A novel method for the labeling of oligonucleotides which results in the economical synthesis of 5′ labeled molecules. A set of suitably protected and carefully selected set of amino linkers, a modified deprotination/cleavage protocol and standard coupling methodologies to are used to allow for the convergent synthesis of any number of labeled oligonucleotides.

Abstract: Fluorescent dyes based on rhodamine are derivatized to form labeled conjugates that fluoresce upon excitation with light of an appropriate wavelength. Particularly preferred embodiments are certain single isomer form rhodamine phosphoramidites. These rhodamine phosphoramidites enhance the efficiency of synthesizing rhodamine-labeled oligonucleotides by solid phase methods. Conjugate embodiments of the invention are prevented from being converted to a non-fluorescent lactam form due to having a fully substituted amide linkage derived from the 3-position carboxylate.

Abstract: A serum-free insect cell culture medium is provided which provides improvements in the maximum insect cell density and replication of insect viruses within these cells, at a significantly lower cost than commercially-available media. A method is provided for preparing a lipid emulsion for use in insect culture media for large scale culture, and which produces a more stable emulsion with a longer shelf life. The method is preferably carried out by combining in an organic solvent a surfactant and a mixture of lipids to make a lipid phase, heating the lipid phase to a temperature of 40 to 70 degrees Celsius to form an anhydrous lipid phase and adding an aqueous phase thereto to provide a stable lipid micro-emulsion.

Abstract: A novel method for the labeling of oligonucleotides which results in the economical synthesis of 5′ labeled molecules. A set of suitably protected and carefully selected set of amino linkers, a modified deprotination/cleavage protocol and standard coupling methodologies to are used to allow for the convergent synthesis of any number of labeled oligonucleotides.

Abstract: A serum-free insect cell culture medium is described which provides improvements in the maximum cell density supported and replication of insect viruses within these cells, at a significantly lower cost than commercially-available media. An improved method for preparing a lipid emulsion which is easy to scale and which produces a more stable emulsion with a longer shelf life is also described.