Abstract: Specific epitopic regions of thyroid peroxidase (TPO), a thyroid specific membrane autoantigen, have been identified within amino acid residues 456 to 933, 457 to 633, 513 to 633, and 633 to 933 of the protein (SEQ ID NO:2), with at least one distinct binding region within TPO located from amino acid residues 592 to 613 (SEQ ID NO:7). The identification and production of these localized epitopes/epitopic regions provide specific therapeutic reagents for administration to autoimmune thyroid disease patients.

Abstract: Specific epitopic regions of thyroid peroxidase (TPO), a thyroid specific membrane autoantigen, have been identified within amino acid residues 456 to 933, 517 to 630, and 633 to 933 of the protein (SEQ ID NO:1), with at least one distinct binding region within TPO located from amino acid residues 592 to 613 (SEQ ID NO:3). The identification and production of these localized epitopes/epitopic regions provide specific diagnostic reagents for autoimmune thyroid disease.

Abstract: Sheep antiserum developed against the human glycohemoglobin, Hb A.sub.1c, distinguishes this fraction from the major component Hb A.sub.o. Partial cross relativity is observed with Hb A.sub.1a and Hb A.sub.1b, as well as with analogous glycohemoglobins from mouse and dog hemolysates. The reactivity of the hemoglobins with this specific antiserum is abolished by the reduction of the keto group of the sugar ligand. The immunological specificity displayed provides the basis of a quantitative assay for Hb A.sub.1c, which is suitable for studies of clinical and experimental diabetes.