Patents by Inventor Ronald Sapolsky
Ronald Sapolsky has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20230340575Abstract: This disclosure provides methods and systems useful in array-based analysis of mixed nucleic acid populations, including for multiplex genotyping of a mixed nucleic acid sample and for detecting differences in copy number of a target polynucleotide and/or a target chromosome (e.g., microdeletions, duplications and aneuploidies). The disclosure also provides methods and systems useful in the diagnosis of genetic abnormalities in a mixed nucleic acid population taken non-invasively from an organism, such as a sample of blood, plasma, serum, urine stool or saliva. The disclosed methods and systems find use in multiple applications, including prenatal testing and cancer diagnostics.Type: ApplicationFiled: December 20, 2022Publication date: October 26, 2023Inventors: Ronald SAPOLSKY, Michael SHAPERO, Jeanette SCHMIDT, Eric FUNG, Orna MIZRAHI MAN, Jiang LI, Monica CHADHA, Anju SHUKLA
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Patent number: 11603557Abstract: This disclosure provides methods and systems useful in array-based analysis of mixed nucleic acid populations, including for multiplex genotyping of a mixed nucleic acid sample and for detecting differences in copy number of a target polynucleotide and/or a target chromosome (e.g., microdeletions, duplications and aneuploidies). The disclosure also provides methods and systems useful in the diagnosis of genetic abnormalities in a mixed nucleic acid population taken non-invasively from an organism, such as a sample of blood, plasma, serum, urine stool or saliva. The disclosed methods and systems find use in multiple applications, including prenatal testing and cancer diagnostics. The disclosure is based on the hybridisation of amplified fragments from the sample, e.g. a maternal sample, which may employ molecular inversion probes MIP to an oligonucleotide array and the detection of the alleles based on different signals from the different alleles of the SNP.Type: GrantFiled: June 1, 2018Date of Patent: March 14, 2023Assignee: Affymetrix, Inc.Inventors: Ronald Sapolsky, Michael Shapero, Jeanette Schmidt, Eric Fung, Orna Mizrahi Man, Jiang Li, Monica Chadha, Anju Shukla
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Publication number: 20230002814Abstract: This disclosure provides methods and kits useful in analysis of mixed nucleic acid populations, including for multiplex genotyping of a mixed nucleic acid sample and for detecting differences in copy number of a target polynucleotide and/or a target chromosome (e.g., microdeletions, duplications, and aneuploidies). The disclosure also provides methods and systems useful in the diagnosis of genetic abnormalities in a mixed nucleic acid population taken non-invasively from an organism, such as a sample of blood, plasma, serum, urine stool or saliva. The disclosed methods and systems find use in multiple applications, including prenatal testing and cancer diagnostics. The method is based on the hybridization of amplified fragments obtained from the sample, e.g., using molecular inversion probes (MIP) to an oligonucleotide array and the detection of the alleles based on different signals from the different alleles of the SNP.Type: ApplicationFiled: September 12, 2022Publication date: January 5, 2023Inventors: Michael SHAPERO, Ronald SAPOLSKY, Eric FUNG, Jeanette SCHMIDT, Monica CHADHA, Anju SHUKLA
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Patent number: 11441174Abstract: This disclosure provides methods and kits useful in analysis of mixed nucleic acid populations, including for multiplex genotyping of a mixed nucleic acid sample and for detecting differences in copy number of a target polynucleotide and/or a target chromosome (e.g., microdeletions, duplications and aneuploidies). The disclosure also provides methods and systems useful in the diagnosis of genetic abnormalities in a mixed nucleic acid population taken non-invasively from an organism, such as a sample of blood, plasma, serum, urine stool or saliva. The disclosed methods and systems find use in multiple applications, including prenatal testing and cancer diagnostics. The method is based on the hybridisation of amplified fragments obtained from the sample, e.g. using molecular inversion probes (MIP) to an oligonucleotide array and the detection of the alleles based on different signals from the different alleles of the SNP.Type: GrantFiled: June 1, 2018Date of Patent: September 13, 2022Inventors: Michael Shapero, Ronald Sapolsky, Eric Fung, Jeanette Schmidt, Monica Chadha, Anju Shukla
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Publication number: 20210147920Abstract: This disclosure provides methods and kits useful in analysis of mixed nucleic acid populations, including for multiplex genotyping of a mixed nucleic acid sample and for detecting differences in copy number of a target polynucleotide and/or a target chromosome (e.g., microdeletions, duplications and aneuploidies). The disclosure also provides methods and systems useful in the diagnosis of genetic abnormalities in a mixed nucleic acid population taken non-invasively from an organism, such as a sample of blood, plasma, serum, urine stool or saliva. The disclosed methods and systems find use in multiple applications, including prenatal testing and cancer diagnostics. The method is based on the hybridisation of amplified fragments obtained from the sample, e.g. using molecular inversion probes (MIP) to an oligonucleotide array and the detection of the alleles based on different signals from the different alleles of the SNP.Type: ApplicationFiled: June 1, 2018Publication date: May 20, 2021Inventors: Michael SHAPERO, Ronald SAPOLSKY, Eric FUNG, Jeanette SCHMIDT, Monica CHADHA, Anju SHUKLA
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Publication number: 20210147919Abstract: This disclosure provides methods and systems useful in array-based analysis of mixed nucleic acid populations, including for multiplex genotyping of a mixed nucleic acid sample and for detecting differences in copy number of a target polynucleotide and/or a target chromosome (e.g., microdeletions, duplications and aneuploidies). The disclosure also provides methods and systems useful in the diagnosis of genetic abnormalities in a mixed nucleic acid population taken non-invasively from an organism, such as a sample of blood, plasma, serum, urine stool or saliva. The disclosed methods and systems find use in multiple applications, including prenatal testing and cancer diagnostics. The disclosure is based on the hybridisation of amplified fragments from the sample, e.g. a maternal sample, which may employ molecular inversion probes MIP to an oligonucleotide array and the detection of the alleles based on different signals from the different alleles of the SNP.Type: ApplicationFiled: June 1, 2018Publication date: May 20, 2021Inventors: Ronald SAPOLSKY, Michael SHAPERO, Jeanette SCHMIDT, Eric FUNG, Orna MIZRAHI MAN, Jiang LI, Monica CHADHA, Anju SHUKLA
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Publication number: 20120258879Abstract: The present invention generally provides a rapid efficient method for analyzing polymorphic or biallelic markers, and arrays for carrying out these analyses. In general, the methods of the present invention employ arrays of oligonucleotide probes that are complementary to target nucleic acids which correspond to the marker sequences of an individual. The probes are typically arranged in detection blocks, each block being capable of discriminating the three genotypes for a given marker, i.e., the heterozygote or either of the two homozygotes.Type: ApplicationFiled: November 15, 2011Publication date: October 11, 2012Applicant: AFFYMETRIX, INC.Inventors: Robert J. Lipshutz, Ronald Sapolsky, Ghassan Ghandour
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Publication number: 20100286924Abstract: The present invention generally provides a rapid efficient method for analyzing polymorphic or biallelic markers, and arrays for carrying out these analyses. In general, the methods of the present invention employ arrays of oligonucleotide probes that are complementary to target nucleic acids which correspond to the marker sequences of an individual. The probes are typically arranged in detection blocks, each block being capable of discriminating the three genotypes for a given marker, i.e., the heterozygote or either of the two homozygotes.Type: ApplicationFiled: January 25, 2010Publication date: November 11, 2010Applicant: AFFYMETRIX, INC.Inventors: ROBERT J. LIPSHUTZ, RONALD SAPOLSKY, GHASSAN GHANDOUR
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Publication number: 20080044920Abstract: The present invention generally provides a rapid efficient method for analyzing polymorphic or biallelic markers, and arrays for carrying out these analyses. In general, the methods of the present invention employ arrays of oligonucleotide probes that are complementary to target nucleic acids which correspond to the marker sequences of an individual. The probes are typically arranged in detection blocks, each block being capable of discriminating the three genotypes for a given marker, i.e., the heterozygote or either of the two homozygotes.Type: ApplicationFiled: February 16, 2007Publication date: February 21, 2008Applicant: Affymetrix, INC.Inventors: Robert Lipshutz, Ronald Sapolsky, Ghassan Ghandour
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Publication number: 20060223097Abstract: The present invention relates to novel methods for sequencing and mapping genetic markers in polynucleotide sequences using Type-IIs restriction endonucleases. The methods herein described result in the “capturing” and determination of specific oligonucleotide sequences located adjacent to Type-IIs restriction sites. The resulting sequences are useful as effective markers for use in genetic napping, screening and manipulation.Type: ApplicationFiled: June 12, 2006Publication date: October 5, 2006Applicant: Affymetrix, INC.Inventors: Ronald Sapolsky, Robert Lipshutz, Thomas Gingeras
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Publication number: 20060110752Abstract: The present invention generally provides a rapid efficient method for analyzing polymorphic or biallelic markers, and arrays for carrying out these analyses. In general, the methods of the present invention employ arrays of oligonucleotide probes that are complementary to target nucleic acids which correspond to the marker sequences of an individual. The probes are typically arranged in detection blocks, each block being capable of discriminating the three genotypes for a given marker, e g, the heterozygote or either of the two homozygotes.Type: ApplicationFiled: October 11, 2005Publication date: May 25, 2006Applicant: Affymetrix, INC.Inventors: Robert Lipshutz, Ronald Sapolsky, Ghassan Ghandour
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Patent number: 6953663Abstract: The present invention generally provides a rapid efficient method for analyzing polymorphic or biallelic markers, and arrays for carrying out these analyses. In general, the methods of the present invention employ arrays of oligonucleotide probes that are complementary to target nucleic acids which correspond to the marker sequences of an individual. The probes are typically arranged in detection blocks, each block being capable of discriminating the three genotypes for a given marker, e.g., the heterozygote or either of the two homozygotes. The method allows for rapid, automatable analysis of genetic linkage to even complex polygenic traits.Type: GrantFiled: June 27, 2003Date of Patent: October 11, 2005Assignee: Affymetrix, Inc.Inventors: Robert J. Lipshutz, Ronald Sapolsky, Ghassan Ghandour
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Publication number: 20050208570Abstract: The invention provides oligonucleotides and their complements that can be used as allele-specific probes or primers for sequencing, oligonucleotide probe hybridization, and allele-specific amplification. Such oligonucleotides can be used, for example, to facilitate genetic distinction between individual plants in plant populations.Type: ApplicationFiled: May 9, 2005Publication date: September 22, 2005Applicant: Affymetrix, Inc.Inventors: Bertrand Lemieux, Benoit Landry, Ronald Sapolsky, Alain Murigneux
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Publication number: 20050026212Abstract: The present invention relates to novel methods for sequencing and mapping genetic markers in polynucleotide sequences using Type-IIs restriction endonucleases. The methods herein described result in the “capturing” and determination of specific oligonucleotide sequences located adjacent to Type-IIs restriction sites. The resulting sequences are useful as effective markers for use in genetic mapping, screening and manipulation.Type: ApplicationFiled: September 16, 2004Publication date: February 3, 2005Applicants: Affymetrix, INC., Affymax Technologies N.V.Inventors: Ronald Sapolsky, Robert Lipshutz, Thomas Gingeras
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Patent number: 6586186Abstract: The present invention generally provides a rapid efficient method for analyzing polymorphic or biallelic markers, and arrays for carrying out these analyses. In general, the methods of the present invention employ arrays of oligonucleotide probes that are complementary to target nucleic acids which correspond to the marker sequences of an individual. The probes are typically arranged in detection blocks, each block being capable of discriminating the three genotypes for a given marker, e.g., the heterozygote or either of the two homozygotes. The method allows for rapid, automatable analysis of genetic linkage to even complex polygenic traits.Type: GrantFiled: August 24, 2001Date of Patent: July 1, 2003Assignee: Affymetrix, Inc.Inventors: Robert J. Lipshutz, Ronald Sapolsky, Ghassan Ghandour
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Publication number: 20020098496Abstract: The present invention generally provides a rapid efficient method for analyzing polymorphic or biallelic markers, and arrays for carrying out these analyses. In general, the methods of the present invention employ arrays of oligonucleotide probes that are complementary to target nucleic acids which correspond to the marker sequences of an individual. The probes are typically arranged in detection blocks, each block being capable of discriminating the three genotypes for a given marker, e.g., the heterozygote or either of the two homozygotes. The method allows for rapid, automatable analysis of genetic linkage to even complex polygenic traits.Type: ApplicationFiled: August 24, 2001Publication date: July 25, 2002Inventors: Robert J. Lipshutz, Ronald Sapolsky, Ghassan Ghandour
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Patent number: 6300063Abstract: The present invention generally provides a rapid efficient method for analyzing polymorphic or biallelic markers, and arrays for carrying out these analyses. In general, the methods of the present invention employ arrays of oligonucleotide probes that are complementary to target nucleic acids which correspond to the marker sequences of an individual. The probes are typically arranged in detection blocks, each block being capable of discriminating the three genotypes for a given marker, e.g., the heterozygote or either of the two homozygotes. The method allows for rapid, automatable analysis of genetic linkage to even complex polygenic traits.Type: GrantFiled: May 8, 1997Date of Patent: October 9, 2001Assignee: Affymetrix, Inc.Inventors: Robert J. Lipshutz, Ronald Sapolsky, Ghassan Ghandour