Patents by Inventor Ronghao Zhou

Ronghao Zhou has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Adenine DNA base editor variants with reduced off-target RNA editing
    Patent number: 11946040
    Abstract: Engineered adenine base editor (ABE) variants with reduced RNA editing activity, and methods of using the same.
    Type: Grant
    Filed: February 4, 2020
    Date of Patent: April 2, 2024
    Assignee: The General Hospital Corporation
    Inventors: J. Keith Joung, Julian Grunewald, Ronghao Zhou
  • C-to-G Transversion DNA Base Editors
    Publication number: 20220411777
    Abstract: Engineered transversion base editors that enable expanded amino acid modifications and methods of using the same. Described herein, for example, are fusion proteins containing cytidine deaminases (e.g. human or rat APOBECs, pmCDA1 or AID) or adenosine deaminases (e.g. E. coli TadAs) or a combination thereof, catalytically impaired CRISPR-Cas proteins (e.g. Cas9, CasX or Cas12 nucleases), linkers, nuclear localization signals (NLSs) and a human or E. coli uracil-n-glycosylase (UNG) and/or REV1 protein that enable the CRISPR-guided programmable introduction of C-to-G and G-to-C transversions in DNA. The UNG may be fused to the deaminase-Cas fusion or not, in which case endogenous UNG may be recruited using molecular machinery that is integrated into the deaminase-Cas fusion architecture, e.g. using peptide or RNA aptamers or scFVs, sdABs or Fabs.
    Type: Application
    Filed: August 31, 2020
    Publication date: December 29, 2022
    Inventors: J. Keith Joung, Ibrahim Cagri Kurt, Ronghao Zhou, Julian Grunewald
  • Combinatorial Adenine and Cytosine DNA Base Editors
    Publication number: 20220290121
    Abstract: Engineered bifunctional adenine and cytosine base editor (BACE) variants that enable expanded amino acid modifications and methods of using the same. Described herein are fusion proteins containing adenosine deaminases, cytidine deaminases, catalytically impaired CRISPR-Cas proteins (e.g., Cas9, CasX or Cas12 nucleases), linkers, nuclear localization signals (NLSs) and uracil-n-glycosylase inhibitors (UGIs) that enable the CRISPR-guided programmable introduction of simultaneous A-to-G (T-to-C) and C-to-T (G-to-A) substitutions in DNA.
    Type: Application
    Filed: August 31, 2020
    Publication date: September 15, 2022
    Inventors: J. Keith Joung, Ronghao Zhou, Julian Grunewald
  • Selective Curbing of Unwanted RNA Editing (SECURE) DNA Base Editor Variants
    Publication number: 20210395730
    Abstract: Engineered base editor variants with reduced RNA editing activity, and methods of using the same.
    Type: Application
    Filed: October 10, 2019
    Publication date: December 23, 2021
    Inventors: Julian Grunewald, J. Keith Joung, Ronghao Zhou
  • ADENINE DNA BASE EDITOR VARIANTS WITH REDUCED OFF-TARGET RNA EDITING
    Publication number: 20200308571
    Abstract: Engineered adenine base editor (ABE) variants with reduced RNA editing activity, and methods of using the same.
    Type: Application
    Filed: February 4, 2020
    Publication date: October 1, 2020
    Inventors: J. Keith Joung, Julian Grunewald, Ronghao Zhou