Abstract: A honeycomb denitration catalyst used for flue gas at 400° C.-600° C. and preparation method thereof. The honeycomb denitration catalyst includes a catalyst coating and a honeycomb ceramic, where a slurry of the catalyst coating is made from components having the following mass percentages: 15%-25% of a zeolite, 5%-10% of a ?-alumina, 5%-10% of a catalyst auxiliary agent, 5% of a binder, and 50%-70% of deionized water. The honeycomb ceramic is soaked repeatedly into the slurry of the catalyst coating. After the soaking is completed, the obtained product is dried and calcined to obtain the honeycomb denitration catalyst. The honeycomb denitration catalyst contains a catalyst auxiliary agent and has excellent denitration activity at high temperatures, sulphur-resistance and water-tolerance ability, stability and NOx removing ability.