Patents by Inventor Ronnie Palmgren
Ronnie Palmgren has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20180223003Abstract: The present invention relates to dimeric pentadentate chelators with exceptionally strong binding of metal ions, for detection, immobilization and purification of biomolecules. Dimeric chelators offer a cooperativity of binding of two adjacent immobilized metal ions simultaneously to a histidine-tagged biomolecule, which gives advantageous properties regarding strength of binding compared to a corresponding monomer chelator. In addition, a dimer increases the selectivity (ease of separation) against non-tagged biomolecules with low metal-ion affinity.Type: ApplicationFiled: March 27, 2018Publication date: August 9, 2018Inventors: Mattias Algotsson, Lars C. Andersson, Eggert Brekkan, Lee Hedemyr, Jean-Luc Maloisel, Ronnie Palmgren
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Patent number: 9989446Abstract: The invention discloses a sample preservation method which comprises the following steps: a) providing a paper substrate comprising ligands, where the ligands comprise charged groups, b) applying a sample comprising at least one analyte and at least one contaminant on the paper substrate, c) drying the sample on the paper substrate and, d) extracting at least part of the paper substrate to provide a solution of the analyte.Type: GrantFiled: February 27, 2012Date of Patent: June 5, 2018Assignee: GE HEALTHCARE UK LIMITEDInventors: Philip Robert Mallender, Douglas Mark Green, Sarah Janet Clegg, Barry Edmund Johnson, Jimmy Hedin Dahlstrom, Mattias Algotsson, Ronnie Palmgren
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Patent number: 9944722Abstract: The present invention relates to dimeric pentadentate chelators with exceptionally strong binding of metal ions, for detection, immobilization and purification of biomolecules. Dimeric chelators offer a cooperativity of binding of two adjacent immobilized metal ions simultaneously to a histidine-tagged biomolecule, which gives advantageous properties regarding strength of binding compared to a corresponding monomer chelator. In addition, a dimer increases the selectivity (ease of separation) against non-tagged biomolecules with low metal-ion affinity.Type: GrantFiled: May 30, 2011Date of Patent: April 17, 2018Assignee: GE Healthcare Bioprocess R&D ABInventors: Mattias Algotsson, Lars C. Andersson, Eggert Brekkan, Lee Hedemyr, Jean-Luc Maloisel, Ronnie Palmgren
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Patent number: 9797871Abstract: The invention relates to a system and method for the stable storage of sensitive biological or chemical target substance, in a bound form on certain capture media. The method comprised providing a sample containing the target substance in a suitable buffer; combining the sample with a capture media to effect reversible binding of the target substance to the capture media; and storing the capture media with the target substance at between about ?20 and 20° C.; and recovering the target substance from the capture media. The target substance recovered maintains the desired activity. Also provides are methods for reducing aggregates in the sensitive biological or chemical target substance.Type: GrantFiled: April 28, 2011Date of Patent: October 24, 2017Assignee: GE Healthcare Bio-Sciences ABInventors: James Van Alstine, Johan Ohman, Philippe Busson, Ronnie Palmgren, Klas Allmer, John Daicic
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Publication number: 20170247407Abstract: The invention discloses a polypeptide capable of binding immunoglobulins or immunoglobulin-containing proteins, which polypeptide comprises six or more domains of protein Z or the C domain of protein A or a functional variant thereof. It also discloses separation matrices comprising the polypeptide and methods of using the separation matrices for separation of immunoglobulins or immunoglobulin-containing proteins.Type: ApplicationFiled: May 16, 2017Publication date: August 31, 2017Applicant: GE Healthcare BioProcess R&D ABInventors: Hans J. Johansson, Ronnie Palmgren
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Patent number: 9657055Abstract: The invention discloses a polypeptide capable of binding immunoglobulins or immunoglobulin-containing proteins, which polypeptide comprises six or more domains of protein Z or the C domain of protein A or a functional variant thereof. It also discloses separation matrices comprising the polypeptide and methods of using the separation matrices for separation of immunoglobulins or immunoglobulin-containing proteins.Type: GrantFiled: November 28, 2012Date of Patent: May 23, 2017Assignee: GE Healthcare BioProcess R&D ABInventors: Hans J. Johansson, Ronnie Palmgren
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Publication number: 20170051010Abstract: The present invention relates to a method for removal of large contaminants, such as virus, in a chromatographic process for purification of a target molecule, preferably monoclonal antibodies, mAbs, by using a specifically designed chromatographic bead having a thin outer layer and a core functionalized with a ligand adsorbing the mAbs or parts thereof.Type: ApplicationFiled: June 4, 2015Publication date: February 23, 2017Inventors: Jean-Luc Maloisel, Ola Lind, Bjorn Noren, Ronnie Palmgren
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Patent number: 9063082Abstract: The invention relates to a method to produce gels for electrophoresis, wherein at least two monomer solutions are printed in a pattern on a substrate and the printed substrate is exposed to electromagnetic or ionising radiation so as to initiate polymerisation. The gels are useful for electrophoretic separation of proteins, peptides and/or nucleic acids.Type: GrantFiled: November 26, 2010Date of Patent: June 23, 2015Assignee: GE HEALTHCARE BIO-SCIENCES ABInventors: Erik Bjerneld, Jan-Olof Johansson, Ronnie Palmgren, Elsemarie Bjellqvist
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Publication number: 20140329995Abstract: The invention discloses a polypeptide capable of binding immunoglobulins or immunoglobulin-containing proteins, which polypeptide comprises six or more domains of protein Z or the C domain of protein A or a functional variant thereof. It also discloses separation matrices comprising the polypeptide and methods of using the separation matrices for separation of immunoglobulins or immunoglobulin-containing proteins.Type: ApplicationFiled: November 28, 2012Publication date: November 6, 2014Applicant: GE HEALTHCARE BIO-SCIENCES ABInventors: Hans J. Johansson, Ronnie Palmgren
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Publication number: 20140302521Abstract: The invention discloses a cellulose or glass fibre paper for preservation of biological samples, which comprises 4-30 wt % of a hydrophilic branched carbohydrate polymer. It also discloses a method for preservation of biological samples by applying and drying them on the paper.Type: ApplicationFiled: October 29, 2012Publication date: October 9, 2014Applicant: GE HEALTHCARE BIO-SCIENCES ABInventors: Mattias Algotsson, Miles W. Burrows, Jimmy Hedin Dahlstrom, Ylva Laurin, Ronnie Palmgren, Jinyu Zou
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Patent number: 8844385Abstract: The present invention relates to a device and method for separation of proteins and other biomolecules. A preferred use is for sample preparation of crude as well as pre-fractionated samples. In a preferred embodiment, the device of the present invention is a pipette tip having dual channels, one for inlet of sample and one for outlet. The outlet, but not the inlet, channel is provided with sample separation media for separation of a desired biomolecule from a sample. The flow through the sample separation media is unidirectional.Type: GrantFiled: April 5, 2013Date of Patent: September 30, 2014Assignee: GE Healthcare Bio-Sciences ABInventors: Nils Norrman, Ronnie Palmgren
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Publication number: 20130330751Abstract: The invention discloses a sample preservation method which comprises the following steps: a) providing a paper substrate comprising ligands, where the ligands comprise charged groups, b) applying a sample comprising at least one analyte and at least one contaminant on the paper substrate, c) drying the sample on the paper substrate and, d) extracting at least part of the paper substrate to provide a solution of the analyte.Type: ApplicationFiled: February 27, 2012Publication date: December 12, 2013Applicant: GE HEALTHCARE UK LIMITEDInventors: Philip Robert Mallender, Douglas Mark Green, Sarah Janet Clegg, Barry Edmund Johnson, Jimmy Hedin Dahlstrom, Mattias Algotsson, Ronnie Palmgren
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Publication number: 20130131323Abstract: The invention relates to a system and method for the stable storage of sensitive biological or chemical target substance, in a bound form on certain capture media. The method comprised providing a sample containing the target substance in a suitable buffer; combining the sample with a capture media to effect reversible binding of the target substance to the capture media; and storing the capture media with the target substance at between about ?20 and 20° C.; and recovering the target substance from the capture media. The target substance recovered maintains the desired activity. Also provides are methods for reducing aggregates in the sensitive biological or chemical target substance.Type: ApplicationFiled: April 28, 2011Publication date: May 23, 2013Applicant: GE HEALTHCARE BIO-SCIENCES ABInventors: James Van Alstine, Johan Ohman, Philippe Busson, Ronnie Palmgren, Klas Allmer, John Daicic
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Patent number: 8413528Abstract: The present invention relates to a device and method for separation of proteins and other biomolecules. A preferred use is for sample preparation of crude as well as pre-fractionated samples. In a preferred embodiment, the device of the present invention is a pipette tip having dual channels, one for inlet of sample and one for outlet. The outlet, but not the inlet, channel is provided with sample separation media for separation of a desired biomolecule from a sample. The flow through the sample separation media is unidirectional.Type: GrantFiled: May 20, 2008Date of Patent: April 9, 2013Assignee: GE Healthcare Bio-Sciences ABInventors: Nils Norrman, Ronnie Palmgren
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Publication number: 20130072638Abstract: The present invention relates to dimeric pentadentate chelators with exceptionally strong binding of metal ions, for detection, immobilization and purification of biomolecules. Dimeric chelators offer a cooperativity of binding of two adjacent immobilized metal ions simultaneously to a histidine-tagged biomolecule, which gives advantageous properties regarding strength of binding compared to a corresponding monomer chelator. In addition, a dimer increases the selectivity (ease of separation) against non-tagged biomolecules with low metal-ion affinity.Type: ApplicationFiled: May 30, 2011Publication date: March 21, 2013Applicant: GE HEALTHCARE BIO-SCIENCES ABInventors: Mattias Algotsson, Lars C. Andersson, Eggert Brekkan, Lee Hedemyr, Jean-Luc Maloisel, Ronnie Palmgren
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Publication number: 20120228121Abstract: The invention relates to a method to produce gels for electrophoresis, wherein at least two monomer solutions are printed in a pattern on a substrate and the printed substrate is exposed to electromagnetic or ionising radiation so as to initiate polymerisation. The gels are useful for electrophoretic separation of proteins, peptides and/or nucleic acids.Type: ApplicationFiled: November 26, 2010Publication date: September 13, 2012Applicant: GE HEALTHCARE BIO-SCIENCES ABInventors: Bengt Bjellqvist, Erik Bjerneld, Jan-Olof Johansson, Ronnie Palmgren
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Patent number: 8263343Abstract: The present invention relates to a method of separating a target from a liquid, which includes providing a polymer in an aqueous liquid, which polymer comprises at least one hydrophobic portion; contacting the polymer-containing liquid with the liquid comprising the target; applying a stimulus to the resulting mixture; and maintaining it until a reversible phase separation is obtained. One phase is polymer-rich and contains target(s) and another phase is polymer-poor. By either maintaining the stimulus, or applying a different stimulus and maintaining it, the polymer-rich phase is transformed into a substantially solid phase.Type: GrantFiled: December 21, 2006Date of Patent: September 11, 2012Assignee: GE Healthcare Bio-Science ABInventors: Elisabeth Hallgren, Ronnie Palmgren, Linda Svensson
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Publication number: 20120149875Abstract: The present invention relates to a method of separating one or more immunoglobulin containing proteins from a liquid. The method includes first contacting the liquid with a separation matrix comprising ligands immobilised to a support; allowing the immunoglobulin containing proteins to adsorb to the matrix by interaction with the ligands; followed by an optional step of washing the adsorbed immunoglobulin containing proteins; and recovering said immunoglobulin containing proteins by contacting the matrix with an eluent which releases the proteins. The method improves upon previous separation methods being that each of the ligands consists essentially of a monomer or dimer SpA or protein Z or a functional variant thereof.Type: ApplicationFiled: January 11, 2010Publication date: June 14, 2012Applicant: GE HEALTHCARE BIO-SCIENCES ABInventors: Hans J. Johansson, Anders Ljunglof, Ronnie Palmgren
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Publication number: 20110114488Abstract: The present invention relates to electrophoresis and in particular low fluorescent electrophoretic supports for hydrogels used for separation of fluorescence labelled biomolecules. More particularly, the invention relates to use of a polymer having the following formula: wherein n=0-100 000 m=0-100 000 R1, R2, R3 and R4=H, F, Cl, Br, I, methyl groups or non-aromatic hydrocarbon chains (optionally containing branches or cyclic structures) such as ethyl, ethenyl, propyl, isopropyl, propenyl, butyl, branched butyl, butenyl, cyclobutyl, pentyl, branched pentyl, pentenyl, cyclopentyl, hexyl, branched hexyl, cyclohexyl; X, Y=methylene groups or non-aromatic hydrocarbon chains (optionally containing branches or cyclic structures) such as ethylene, ethenylene, propylene, isopropylene, propenylene, butylene, branched butylene, butenylene; Y can optionally be absent as a low fluorescent support film of an electrophoretic hydrogel for slab gel electrophoresis.Type: ApplicationFiled: January 25, 2011Publication date: May 19, 2011Applicant: GE HEALTHCARE BIO-SCIENCES ABInventors: ANDERS LARSSON, RONNIE PALMGREN, SOFIA SÖDERBERG
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Publication number: 20100126255Abstract: The present invention relates to a device and method for separation of proteins and other biomolecules. A preferred use is for sample preparation of crude as well as pre-fractionated samples. In a preferred embodiment, the device of the present invention is a pipette tip having dual channels, one for inlet of sample and one for outlet. The outlet, but not the inlet, channel is provided with sample separation media for separation of a desired biomolecule from a sample. The flow through the sample separation media is unidirectional.Type: ApplicationFiled: May 20, 2008Publication date: May 27, 2010Applicant: GE HEALTHCARE BIO-SCIENCE ABInventors: Nils Norrman, Ronnie Palmgren