Patents by Inventor Rosemary CLARK
Rosemary CLARK has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20240010576Abstract: Disclosed herein are methods of increasing nitrogen fixation in a non-leguminous plant. The methods can comprise exposing the plant to a plurality of bacteria. Each member of the plurality comprises one or more genetic variations introduced into one or more genes or non-coding polynucleotides of the bacteria's nitrogen fixation or assimilation genetic regulatory network, such that the bacteria are capable of fixing atmospheric nitrogen in the presence of exogenous nitrogen. The bacteria are not intergeneric microorganisms. Additionally, the bacteria, in planta, produce 1% or more of the fixed nitrogen in the plant.Type: ApplicationFiled: June 30, 2023Publication date: January 11, 2024Applicant: Pivot Bio, Inc.Inventors: Karsten Temme, Alvin Tamsir, Sarah Bloch, Rosemary Clark, Emily Tung
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Patent number: 11739032Abstract: Disclosed herein are methods of increasing nitrogen fixation in a non-leguminous plant. The methods can comprise exposing the plant to a plurality of bacteria. Each member of the plurality comprises one or more genetic variations introduced into one or more genes or non-coding polynucleotides of the bacteria's nitrogen fixation or assimilation genetic regulatory network, such that the bacteria are capable of fixing atmospheric nitrogen in the presence of exogenous nitrogen. The bacteria are not intergeneric microorganisms. Additionally, the bacteria, in planta, produce 1% or more of the fixed nitrogen in the plant.Type: GrantFiled: January 13, 2021Date of Patent: August 29, 2023Assignee: Pivot Bio, Inc.Inventors: Karsten Temme, Alvin Tamsir, Sarah Bloch, Rosemary Clark, Emily Tung
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Patent number: 11565979Abstract: Methods and systems are provided for generating and utilizing a bacterial composition that comprises at least one genetically engineered bacterial strain that fixes atmospheric nitrogen in an agricultural system that has been fertilized with more than 20 lbs of Nitrogen per acre.Type: GrantFiled: November 15, 2018Date of Patent: January 31, 2023Assignee: Pivot Bio, Inc.Inventors: Karsten Temme, Alvin Tamsir, Sarah Bloch, Rosemary Clark, Emily Tung, Kevin Hammill, Douglas Higgins, Austin Davis-Richardson
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Publication number: 20220396530Abstract: The present disclosure provides non-intergeneric remodeled microbes that are able to fix atmospheric nitrogen and deliver such to plants in a targeted, efficient, and environmentally sustainable manner. The utilization of the taught microbial products will enable farmers to realize more productive and predictable crop yields without the nutrient degradation, leaching, or toxic runoff associated with traditional synthetically derived nitrogen fertilizer, by mitigating or eliminating the need for exogenous nitrogen-containing fertilizers. The remodeled microbes have unique colonization and nitrogen fixation abilities, which enable the microbes to deliver nitrogen to a cereal plant in a spatially targeted (e.g. rhizospheric) and temporally targeted (e.g. during advantageous stages of plants life cycle) manner. The microbes are able to replace the standard agricultural practice of sidedressing and enable a more environmentally sustainable form of farming.Type: ApplicationFiled: July 11, 2019Publication date: December 15, 2022Inventors: Alvin TAMSIR, Sarah BLOCH, Mark REISINGER, Ernest SANDERS, Richard BROGLIE, Rosemary CLARK, Karsten TEMME
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Publication number: 20220127627Abstract: The present disclosure provides guided microbial remodeling (GMR) methods for the rational improvement of plant-associated microbes to perform plant-beneficial functions. The GMR methods described herein allow for non-intergeneric genetic optimization of key regulatory networks within the microbes, which improve plant-beneficial functions over wild-type microbes but don't have the risks associated with transgenic approaches (e.g., unpredictable gene function, public and regulatory concerns, etc.). The present disclosure also provides remodeled microbes and compositions thereof. The utilization of remodeled microbes and compositions thereof will enable farmers to realize more productive and predictable crop yields without the nutrient degradation, leaching, or toxic runoff associated with traditional synthetically derived fertilizers.Type: ApplicationFiled: June 27, 2019Publication date: April 28, 2022Inventors: Sarah BLOCH, Karsten TEMME, Alvin TAMSIR, Douglas HIGGINS, Austin DAVIS-RICHARDSON, Rosemary CLARK, Shayin GOTTLIEB
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Publication number: 20220017911Abstract: Methods of predicting in planta phenotypes of microbial strains are provided. The methods can include culturing microbial strains in a plant exudate medium and assaying in vitro phenotypes of the microbial strains. The methods may also include using the in vitro phenotypes to predict in planta phenotypes of the microbial strains. Methods of using the microbial strains in field trials are also provided.Type: ApplicationFiled: December 20, 2019Publication date: January 20, 2022Inventors: Karsten Temme, Alvin Tamsir, Sarah Bloch, Neal Shah, Shayin Gottlieb, Richard Broglie, Emily Tung, Rosemary Clark
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Publication number: 20210345618Abstract: Methods and systems are provided for increasing the phosphate solubilization activity of a microbe. Microbes identified through the use of the disclosed methods and systems are also provided. The methods include the steps of and the systems include instructions for identifying a coding sequence associated with phosphate solubilization, altering the coding sequence associated with phosphate solubilization, functionally linking the altered coding sequence associated with phosphate solubilization to a promoter, and introducing the altered coding sequence associated with phosphate solubilization under the control of the promoter into the microbe. Alterations to the coding sequence can include, e.g., codon randomization of native codons, deletion of coding sequence, and insertion of regulatory sequences.Type: ApplicationFiled: September 19, 2019Publication date: November 11, 2021Inventors: Sarah Bloch, Karsten Temme, Alvin Tamsir, Douglas Higgins, Austin Davis-Richardson, Rosemary Clark, Shayin Gottlieb, James Gerard Lorigan
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Publication number: 20210214282Abstract: Disclosed herein are methods of increasing nitrogen fixation in a non-leguminous plant. The methods can comprise exposing the plant to a plurality of bacteria. Each member of the plurality comprises one or more genetic variations introduced into one or more genes or non-coding polynucleotides of the bacteria's nitrogen fixation or assimilation genetic regulatory network, such that the bacteria are capable of fixing atmospheric nitrogen in the presence of exogenous nitrogen. The bacteria are not intergeneric microorganisms. Additionally, the bacteria, in planta, produce 1% or more of the fixed nitrogen in the plant.Type: ApplicationFiled: January 13, 2021Publication date: July 15, 2021Inventors: Karsten Temme, Alvin Tamsir, Sarah Bloch, Rosemary Clark, Emily Tung
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Patent number: 10934226Abstract: Disclosed herein are methods of increasing nitrogen fixation in a non-leguminous plant. The methods can comprise exposing the plant to a plurality of bacteria. Each member of the plurality comprises one or more genetic variations introduced into one or more genes or non-coding polynucleotides of the bacteria's nitrogen fixation or assimilation genetic regulatory network, such that the bacteria are capable of fixing atmospheric nitrogen in the presence of exogenous nitrogen. The bacteria are not intergeneric microorganisms. Additionally, the bacteria, in planta, produce 1% or more of the fixed nitrogen in the plant.Type: GrantFiled: April 16, 2018Date of Patent: March 2, 2021Assignee: Pivot Bio, Inc.Inventors: Karsten Temme, Alvin Tamsir, Sarah Bloch, Rosemary Clark, Emily Tung
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Patent number: 10919814Abstract: Disclosed herein are methods of increasing nitrogen fixation in a non-leguminous plant. The methods can comprise exposing the plant to a plurality of bacteria. Each member of the plurality comprises one or more genetic variations introduced into one or more genes or non-coding polynucleotides of the bacteria's nitrogen fixation or assimilation genetic regulatory network, such that the bacteria are capable of fixing atmospheric nitrogen in the presence of exogenous nitrogen. The bacteria are not intergeneric microorganisms. Additionally, the bacteria, in planta, produce 1% or more of the fixed nitrogen in the plant.Type: GrantFiled: November 15, 2019Date of Patent: February 16, 2021Assignee: Pivot Bio, Inc.Inventors: Karsten Temme, Alvin Tamsir, Sarah Bloch, Rosemary Clark, Emily Tung
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Publication number: 20210009483Abstract: Methods and systems are provided for generating and utilizing a bacterial composition that comprises at least one genetically engineered bacterial strain that fixes atmospheric nitrogen in an agricultural system that has been fertilized with more than 20 lbs of Nitrogen per acre.Type: ApplicationFiled: September 21, 2020Publication date: January 14, 2021Inventors: Karsten Temme, Alvin Tamsir, Sarah Bloch, Rosemary Clark, Emily Tung, Kevin Hammill, Douglas Higgins, Austin Davis-Richardson
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Publication number: 20200087221Abstract: Disclosed herein are methods of increasing nitrogen fixation in a non-leguminous plant. The methods can comprise exposing the plant to a plurality of bacteria. Each member of the plurality comprises one or more genetic variations introduced into one or more genes or non-coding polynucleotides of the bacteria's nitrogen fixation or assimilation genetic regulatory network, such that the bacteria are capable of fixing atmospheric nitrogen in the presence of exogenous nitrogen. The bacteria are not intergeneric microorganisms. Additionally, the bacteria, in planta, produce 1% or more of the fixed nitrogen in the plant.Type: ApplicationFiled: November 15, 2019Publication date: March 19, 2020Inventors: Karsten TEMME, Alvin TAMSIR, Sarah BLOCH, Rosemary CLARK, Emily TUNG
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Patent number: 10556839Abstract: Disclosed herein are methods of increasing nitrogen fixation in a non-leguminous plant. The methods can comprise exposing the plant to a plurality of bacteria. Each member of the plurality comprises one or more genetic variations introduced into one or more genes or non-coding polynucleotides of the bacteria's nitrogen fixation or assimilation genetic regulatory network, such that the bacteria are capable of fixing atmospheric nitrogen in the presence of exogenous nitrogen. The bacteria are not intergeneric microorganisms. Additionally, the bacteria, in planta, produce 1% or more of the fixed nitrogen in the plant.Type: GrantFiled: April 16, 2018Date of Patent: February 11, 2020Assignee: Pivot Bio, Inc.Inventors: Karsten Temme, Alvin Tamsir, Sarah Bloch, Rosemary Clark, Emily Tung
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Patent number: 10384983Abstract: Disclosed herein are methods of increasing nitrogen fixation in a non-leguminous plant. The methods can comprise exposing the plant to a plurality of bacteria. Each member of the plurality comprises one or more genetic variations introduced into one or more genes or non-coding polynucleotides of the bacteria's nitrogen fixation or assimilation genetic regulatory network, such that the bacteria are capable of fixing atmospheric nitrogen in the presence of exogenous nitrogen. The bacteria are not intergeneric microorganisms. Additionally, the bacteria, in planta, produce 1% or more of the fixed nitrogen in the plant.Type: GrantFiled: April 11, 2018Date of Patent: August 20, 2019Assignee: Pivot Bio, Inc.Inventors: Karsten Temme, Alvin Tamsir, Sarah Bloch, Rosemary Clark, Emily Tung
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Publication number: 20190144352Abstract: Methods and systems are provided for generating and utilizing a bacterial composition that comprises at least one genetically engineered bacterial strain that fixes atmospheric nitrogen in an agricultural system that has been fertilized with more than 20 lbs of Nitrogen per acre.Type: ApplicationFiled: November 15, 2018Publication date: May 16, 2019Inventors: Karsten Temme, Alvin Tamsir, Sarah Bloch, Rosemary Clark, Emily Tung, Kevin Hammill, Douglas Higgins, Austin Davis-Richardson
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Publication number: 20190039964Abstract: Methods and systems are provided for generating and utilizing a bacterial composition that comprises at least one genetically engineered bacterial strain that fixes atmospheric nitrogen in an agricultural system that has been fertilized with more than 20 lbs of Nitrogen per acre.Type: ApplicationFiled: October 12, 2018Publication date: February 7, 2019Inventors: Karsten Temme, Alvin Tamsir, Sarah Bloch, Rosemary Clark, Emily Tung, Kevin Hammill, Douglas Higgins, Austin Davis-Richardson
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Publication number: 20180297905Abstract: Disclosed herein are methods of increasing nitrogen fixation in a non-leguminous plant. The methods can comprise exposing the plant to a plurality of bacteria. Each member of the plurality comprises one or more genetic variations introduced into one or more genes or non-coding polynucleotides of the bacteria's nitrogen fixation or assimilation genetic regulatory network, such that the bacteria are capable of fixing atmospheric nitrogen in the presence of exogenous nitrogen. The bacteria are not intergeneric microorganisms. Additionally, the bacteria, in planta, produce 1% or more of the fixed nitrogen in the plant.Type: ApplicationFiled: April 16, 2018Publication date: October 18, 2018Inventors: Karsten TEMME, Alvin TAMSIR, Sarah BLOCH, Rosemary CLARK, Emily TUNG
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Publication number: 20180297906Abstract: Disclosed herein are methods of increasing nitrogen fixation in a non-leguminous plant. The methods can comprise exposing the plant to a plurality of bacteria. Each member of the plurality comprises one or more genetic variations introduced into one or more genes or non-coding polynucleotides of the bacteria's nitrogen fixation or assimilation genetic regulatory network, such that the bacteria are capable of fixing atmospheric nitrogen in the presence of exogenous nitrogen. The bacteria are not intergeneric microorganisms. Additionally, the bacteria, in planta, produce 1% or more of the fixed nitrogen in the plant.Type: ApplicationFiled: April 16, 2018Publication date: October 18, 2018Inventors: Karsten TEMME, Alvin TAMSIR, Sarah BLOCH, Rosemary CLARK, Emily TUNG
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Publication number: 20180273437Abstract: Disclosed herein are methods of increasing nitrogen fixation in a non-leguminous plant. The methods can comprise exposing the plant to a plurality of bacteria. Each member of the plurality comprises one or more genetic variations introduced into one or more genes or non-coding polynucleotides of the bacteria's nitrogen fixation or assimilation genetic regulatory network, such that the bacteria are capable of fixing atmospheric nitrogen in the presence of exogenous nitrogen. The bacteria are not intergeneric microorganisms. Additionally, the bacteria, in planta, produce 1% or more of the fixed nitrogen in the plant.Type: ApplicationFiled: April 11, 2018Publication date: September 27, 2018Inventors: Karsten TEMME, Alvin TAMSIR, Sarah BLOCH, Rosemary CLARK, Emily TUNG
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Patent number: 9975817Abstract: Disclosed herein are methods of increasing nitrogen fixation in a non-leguminous plant. The methods can comprise exposing the plant to a plurality of bacteria. Each member of the plurality comprises one or more genetic variations introduced into one or more genes or non-coding polynucleotides of the bacteria's nitrogen fixation or assimilation genetic regulatory network, such that the bacteria are capable of fixing atmospheric nitrogen in the presence of exogenous nitrogen. The bacteria are not intergeneric microorganisms. Additionally, the bacteria, in planta, produce 1% or more of the fixed nitrogen in the plant.Type: GrantFiled: June 28, 2017Date of Patent: May 22, 2018Assignee: Pivot Bio, Inc.Inventors: Karsten Temme, Alvin Tamsir, Sarah Bloch, Rosemary Clark, Emily Tung