Abstract: Methods, devices, systems, and kits for electro-mechanical cell transfection are provided. A device includes a first electrode, a second electrode, and an active zone therebetween where an electrical potential difference applied to the first and second electrodes generates an electric field in the active zone sufficient to transfect at least a subset of the cells flowing in the active zone.

Abstract: Devices, systems, and kits for cell transfection are provided. A device includes a first electrode, a second electrode, and an electroporation zone therebetween where an electrical potential difference applied to the first and second electrodes generates an electric field in the electroporation zone sufficient to transfect at least a subset of the cells in the flow path. Methods of introducing a composition into at least a portion of a plurality of cells using the devices, systems, and kits of the invention are also provided.

Abstract: Described herein are systems, methods and computer program products for real time assessment and monitoring of biological cells or their environment. In the systems and methods, a computer receives readouts of the biological cells or their environment and assesses changes in the biological cells or their environment by comparing current and previous values of parameters that describe the biological cells or their environment. The computer then determines treatments to be applies to the cells based on the assessing and user-specified criteria and then applies the treatment by controlling a setting on an environmental control unit to cause the treatment to be applied to the biological cells. The systems and methods described herein provide an automated system that includes computer-driven modifications that eliminate the need for the researcher to be present and controlling the flow of the experiment as it is being run.