Abstract: In various embodiments method are provided for generating expression cassettes and gene therapy vectors comprising those cassettes that recapitulate the spatiotemporal pattern of expression of the endogenous gene. In certain embodiments the methods comprise (i) selecting a target gene; (ii) identifying putative regulatory elements associated with the target gene; (iii) determining if the regulatory element is a key regulatory element and (iv) providing a list of the key regulatory elements identified in step (iii).

Abstract: Described herein are transcriptional relay systems useful for reducing background signal in protein expression and reporter assays. These systems utilize a nucleic acid system wherein a promoter sequence controls expression of a synthetic transcription factor that activates transcription of a reporter molecule.

Abstract: Described herein are methods and systems useful for interrogating protein-protein interactions. These systems utilize non-functional fragments of polypeptides that can complement each other to form a fully functional fragment in vivo. The fully functional polypeptide releases a transcription regulating polypeptide that can bind and activate or repress a reporter element.

Abstract: In certain embodiments a lentiviral vector having optimized reduced size LCR with improved enhancer activity is provided. In certain embodiments direct treatment of a subject by direct introduction of the vector(s) described herein is contemplated. The lentiviral compositions may be formulated for delivery by any available route including, but not limited to parenteral (e.g., intravenous), intradermal, subcutaneous, oral (e.g., inhalation), transdermal (topical), transmucosal, rectal, and vaginal.

Abstract: A multicasting download-to-mobile-device service that broadcasts and distributes digital media over cellular networks is described. The most preferred embodiments of the present invention comprise a network that distributes a plurality of media titles (e.g., movies, tv shows, concerts, etc.) via multicast streams where the media titles are stored in local storage on a mobile electronic device (e.g., cellular telephones and tablets) and, by way of a mobile device app, allows users to play the stored media titles locally from their devices using current on demand playback methodologies. In addition, at least some preferred embodiments of the present invention comprise a data carousel that curates and delivers discrete media titles to selected cell towers and/or hubs, thereby providing for relevant media titles to be multicast to discrete regions and not the entire network. Finally, the data carousel may be configured to synchronize relevant titles for discrete users of the service.

Abstract: The present invention relates to a computer-implemented of controlling an electronic device with a display and a user input.

Abstract: Various embodiments provide for an extrusion device with a dummy block having a curved surface configured to change the shape and length of charge welds between extrudates originating from different billets in order to reduce the amount of scrap and waste material. In an embodiment, the dummy block, which forces the billet of material through the die, can have a concave front surface such that the material, as it is extruded through the die, forms a solid-state weld that is shorter than a flat faced dummy block. In another embodiment, the dummy block can have a convex front surface such that the material, as it is extruded through the die, forms a solid-state weld that is longer than a weld due to a flat faced dummy block. In other embodiments, billets with non-flat ends can also be used.

Abstract: In certain embodiments a recombinant retroviral vector is provided where the vector comprises a human ubiquitin C (UBC) promoter operably linked to a transgene where the promoter and the transgene are in a reverse orientation so that the direction of transcription of the transgene from the promoter is oriented towards a 5? long terminal repeat (LTR) of the vector.

Abstract: A method and system for a platform wide party system that may be deployed in a multiplayer gaming environment. Players may associate themselves with one or more parties, wherein players within a party may choose to automatically exchange messages associated with changes in state of the players in a gaming environment. The party system exposes an API providing a plurality of function calls that may be integrated seamlessly with game play. A first player may indicate desired notification events to be received from a second player associated with the same party as the first player. Upon detection of a desired notification event on the video game console of the second player, an event notification may be communicated to a video game console of the first player causing an action to be performed on the first player's video game console.

Abstract: A method and system for a platform wide party system that may be deployed in a multiplayer gaming environment. Players may associate themselves with one or more parties, wherein players within a party may choose to automatically exchange messages associated with changes in state of the players in a gaming environment. The party system exposes an API providing a plurality of function calls that may be integrated seamlessly with game play. A first player may indicate desired notification events to be received from a second player associated with the same party as the first player. Upon detection of a desired notification event on the video game console of the second player, an event notification may be communicated to a video game console of the first player causing an action to be performed on the first player's video game console.

Abstract: A system for financing of film and media productions of individual movies or slates of movies which provides non-monetary enhancements to return on investment for subscribing members who may be television and media broadcasters such as cable systems and satellite broadcasters. The system enhanced return on investment such as earlier broadcast rights and advertisement availments for subscribing members as well as monetary return from revenues.

Abstract: A method of communicating targeted advertising to media reception devices of individual consumers, receiving electronic signals of said media from broadcasters through communication to a local system provider such as a cable or satellite television system provider. The system allows a single broadcaster to disseminate targeted advertising throughout multiple connected system providers to allow centralized target marketing to consumers.

Abstract: A digital coupon system for electronically distributing coupons and inducements to viewers over broadcast media. The system employs a viewable or audible signal to indicate to viewers the availability of a coupon or inducement during real time viewing using an image animation overlaid onto displayed ad or program content. Coupon inducements and delivery can be based on coupon business rules defining requirements for delivery. The system is especially well configured to encourage the viewing of broadcast media commercials which are frequently skipped over by viewers.

Abstract: A multi-source bridge content distribution system links multiple content owners with access network operators or content distribution providers leasing space on access networks so that multi-media content can be provided from multiple content owners to consumers through a multi-source bridge or data center. Content files and associated content owner preference settings are provided from a plurality of content sources or providers to the multi-source data center. Files stored at the data center or locally at an access network are provided to subscribers through the local access network Content files are provided if the content owner preference settings are a sufficient match with service provider access network preference settings set up by the service provider using the access network to provide content to subscribers.

Abstract: In a network user authentication system, a network user is identified for authentication purposes using the unique identifier for a dedicated physical communication line associated with the building in which the network user is located or a digital certificate which is associated with a secure component or communication line physically attached to a building. An authentication server initially verifies the identification of the dedicated communication line to be associated with a network service subscriber or issues a unique digital certificate to be associated with the dedicated communication line for authentication purposes. The digital certificate may be stored in a building gateway or in an edge site module which is connected to the secure components of a plurality of buildings and stores unique digital certificates for each building.

Abstract: Digital content files containing entertainment media such as movies, TV shows, and the like are distributed from at least one content source primarily responsible for the original creation of the media to subscribers over a network system. The content source is connected over at least one content source network to a plurality of distribution partners, and each distribution partner is connected with a selected group of subscribers over an access network. A requested digital content file is distributed from the content source to a subscriber in a network distribution path comprising at least the content source network, the distribution partner associated with the subscriber, and the second network. A profile information package containing subscriber preferences is transmitted from the subscriber to the content source by way of a proxy over the network distribution path, and the profile information package is stored at the content source.

Abstract: A method and system for providing highly targeted advertising to remotely located viewers. The viewers are matched to an identifiable receiver on the system and, based on user preferences for products and services developed and stored on each user, targeted advertising for products and services each said user is likely to want, is substituted for the broadcaster advertising and communicated to the individual receivers matched with the user.

Abstract: In a perfect world, citizens would have all of the sensing equipment and analysis capability with no concern about costs. Unfortunately, the costs are an impediment to most people and their health and well-being may be compromised as a result. By leveraging popular consumer devices such as mobile players and other CE devices, it is possible to create “piggy-back” solutions that effectively bring the costs into the range that ordinary people can afford. Once ordinary people can afford these solutions, the state of their health and/or well being can be improved greatly.

Abstract: A method and device for the provision of enhanced media content distribution over a network. The method enables content providers, advertisers, and consumers to benefit from targeted advertising and promotions. The precise targeting for advertising and promotions are gleaned from consumer information provided by the system as to their geographic location which may be related to other known and mined information about the consumer.

Abstract: A method for the provision of a hybrid premium television platform using terrestrial broadcasting. The system allows for enhanced services to subscribers using a terrestrial broadcast signal. The enhanced services are similar to those enjoyed by more expensive providers such as cable and satellite television providers.