Abstract: A device for sensing analyte concentration, and in particular glucose concentration, in vivo or in vitro is disclosed. A sensing element is attached to the distal end of an optical conduit, and comprises at least one binding protein adapted to bind with at least one target analyte. The sensing element further comprises at least one reporter group that undergoes a luminescence change with changing analyte concentrations. Optionally, the optical conduit and sensing element may be housed within a cannulated bevel.

Abstract: An optical connector device is disclosed for optically connecting a biosensor wearable on the body of a patient to a reader outside of the body of the patient. The device comprises a base disk to be worn on the body of a patient. The base disk has a needle attached thereto and the needle houses a first fiber defining a first optical conduit. The distal end of the needle is insertable into the body of the patient, and a portion of the proximal end of the needle remains outside of the body of the patient when the device is worn. The device also comprises a connector housing a second fiber defining a second optical conduit.

Abstract: A device for sensing analyte concentration, and in particular glucose concentration, in vivo or in vitro is disclosed. A sensing element is attached to the distal end of an optical conduit, and comprises at least one binding protein adapted to bind with at least one target analyte. The sensing element further comprises at least one reporter group that undergoes a luminescence change with changing analyte concentrations. Optionally, the optical conduit and sensing element may be housed within a cannulated bevel.

Abstract: Methods for preparing articles having a bioactive surface comprising treating a substrate to form free reactive groups, depositing a monomer onto the treated substrate, and covalently immobilizing a biologically functional molecule onto the deposited monomer. Additional embodiments include methods for the deposition of the monomer onto the treated substrate in a solvent-free environment. Further embodiments include articles having surfaces prepared using the methods described herein. Additional embodiments include articles prepared using the methods described herein.

Abstract: A device for sensing analyte concentration, and in particular glucose concentration, in vivo or in vitro is disclosed. A sensing element is attached to the distal end of an optical conduit, and comprises at least one binding protein adapted to bind with at least one target analyte. The sensing element further comprises at least one reporter group that undergoes a luminescence change with changing analyte concentrations. Optionally, the optical conduit and sensing element may be housed within a cannulated bevel.

Abstract: An optical connector device is disclosed for optically connecting a biosensor wearable on the body of a patient to a reader outside of the body of the patient. The device comprises a base disk to be worn on the body of a patient. The base disk has a needle attached thereto and the needle houses a first fiber defining a first optical conduit. The distal end of the needle is insertable into the body of the patient, and a portion of the proximal end of the needle remains outside of the body of the patient when the device is worn. The device also comprises a connector housing a second fiber defining a second optical conduit.

Abstract: Multicoated or multilayer matrices capable of embedding, encapsulating or entrapping binding proteins, optionally with a reporter group attached, specific for analytes of interest, in particular sugars, and methods of making and using such matrices.