Abstract: A fluorescent water-soluble polycationic chitosan polymer having a general formula (I) and salts thereof: wherein a is an integer between 220 and 11,200; R1 and R2 are independently selected from the group consisting of CH3CO and H, provided that at least 25% of the O-positions of the chitosan polymer structure is substituted with CH3CO; R7 is selected from the group consisting of CH3CO, H and Dye, provided that (i) at least one Dye residue is present in the chitosan polymer structure, (ii) and at least 15% of the N-positions of the chitosan polymer structure is substituted with CH3CO; Dye is a polymethine dye, having a general formula (II): wherein W represents 4-8 carbon atoms forming a benzo-condensed or a naphto-condensed ring; R3 and R4 are independently selected from the group consisting of CH2CH3, (CH2)3SO3?, (CH2)3N+(CH3)3; R5 and R6 are independently selected from the group consisting of H, SO3?; Q is selected from the group consisting of wherein D is selected from the group

Abstract: A method for increasing the light emission produced by the chemiluminescent reaction of 8-amino-5-chloro-7-phenylpyrido[3,4-d]pyridazine-1,4(2H,3H)-dione, a peroxidase enzyme or a conjugate thereof, an enhancer, a co-enhancer and a peroxide oxidizer, wherein the enhancer is an anionic N-alkylphenothiazine and the co-enhancer is selected from a 4-dialkylaminopyridine or an N-azole, and wherein the method comprises the following steps: i. realizing a chemiluminescent substrate by means of mixing together 8-amino-5-chloro-7-phenylpyrido[3,4-d]pyridazine-1,4(2H,3H)-dione, the enhancer, the co-enhancer and the peroxide oxidizer, and ii. adding the peroxidase enzyme or a conjugate thereof to the chemiluminescent substrate.

Abstract: A method for increasing the light emission produced by the chemiluminescent reaction of 8-amino-5-chloro-7-phenylpyrido[3,4-d]pyridazine-1,4(2H,3H)-dione, a peroxidase enzyme or a conjugate thereof, an enhancer, a co-enhancer and a peroxide oxidizer, wherein the enhancer is an anionic N-alkylphenothiazine and the co-enhancer is selected from a 4-dialkylaminopyridine or an N-azole, and wherein the method comprises the following steps: i. realizing a chemiluminescent substrate by means of mixing together 8-amino-5-chloro-7-phenylpyrido[3,4-d]pyridazine-1,4(2H,3H)-dione, the enhancer, the co-enhancer and the peroxide oxidizer, and ii. adding the peroxidase enzyme or a conjugate thereof to the chemiluminescent substrate.

Abstract: A kit for performing an assay for determining an analyte in a sample with an extended shelf-life, wherein the kit comprises a chemiluminescent cyclic dihydrazide, an enhancer, a co-enhancer, and a peroxide oxidizer. The kit is useful in blot assays and immunoassays for the detection of proteins and nucleic acid molecules.

Abstract: The invention relates to novel tricarbocyanine-cyclodextrin(s) conjugates useful as markers in the diagnosis of kidney diseases, diagnostic compositions comprising the conjugates, their use, and their production.

Abstract: The present invention relates to novel tricarbocyanine-cyclodextrin(s) conjugates useful as markers in the diagnosis of kidney diseases, a diagnostic composition comprising said conjugates, their use and their production.

Abstract: A kit for performing an assay for determining an analyte in a sample with an extended shelf-life, wherein the kit comprises a chemiluminescent cyclic dihydrazide, an enhancer, a co-enhancer, and a peroxide oxidizer. The kit is useful in blot assays and immunoassays for the detection of proteins and nucleic acid molecules.

Abstract: A method for increasing the light emission produced by the chemiluminescent reaction of 8-amino-5-chloro-7-phenylpyrido[3,4-d]pyridazine-1,4(2H,3H)-dione, a peroxidase enzyme or a conjugate thereof, an enhancer, a co-enhancer and a peroxide oxidizer, wherein the enhancer is an anionic N-alkylphenothiazine and the co-enhancer is selected from a 4-dialkylaminopyridine or an N-azole, and wherein the method comprises the following steps: i. realizing a chemiluminescent substrate by means of mixing together 8-amino-5-chloro-7-phenylpyrido[3,4-d]pyridazine-1,4(2H,3H)-dione, the enhancer, the co-enhancer and the peroxide oxidizer, and ii. adding the peroxidase enzyme or a conjugate thereof to the chemiluminescent substrate.

Abstract: The present invention relates to novel tricarbocyanine-cyclodextrin(s) conjugates useful as markers in the diagnosis of kidney diseases, a diagnostic composition comprising said conjugates, their use and their production.

Abstract: The present invention relates to novel tricarbocyanine-cyclodextrin(s) conjugates useful as markers in the diagnosis of kidney diseases, a diagnostic composition comprising said conjugates, their use and their production.

Abstract: The present invention describes novel 7-alkylamino-3-(thienyl) coumarin fluorescent dyes of formula (I) These dyes are water soluble, can be excited by the 405 nm excitation source and exhibit a large Stokes shift (?80 nm). Furthermore, the dyes possess a reactive group for the labeling of biomolecules or other analytes.

Abstract: The present invention describes novel 7-alkylamino-3-(thienyl) coumarin fluorescent dyes of formula (I) These dyes are water soluble, can be excited by the 405 nm excitation source and exhibit a large Stokes shift (?80 nm). Furthermore, the dyes possess a reactive group for the labeling of biomolecules or other analytes.

Abstract: The present invention relates to novel tricarbocyanine-cyclodextrin(s) conjugates useful as markers in the diagnosis of kidney diseases, a diagnostic composition comprising said conjugates, their use and their production.

Abstract: Method for increasing and regulating the emission of light from a chemiluminescent reaction including luminol, a peroxidase enzyme, an oxidant and an electron mediator (primary enhancer) through the use of an acylation catalyst (secondary enhancer) belonging to the class of N-azoles, i.e., a class of five-membered nitrogen heteroaromatic ring compounds containing at least one other atom of nitrogen. N-azoles, which are especially useful as secondary enhancers are imidazole, 1-methylimidazole, 1,2,3-triazole and 1,2,4-triazole. The invention also describes the use in diagnostic assays of chemiluminescent substrates containing said N-azoles, as secondary enhancers.

Abstract: Method for increasing and regulating the emission of light from a chemiluminescent reaction including luminol, a peroxidase enzyme, an oxidant and an electron mediator (primary enhancer) through the use of an acylation catalyst (secondary enhancer) belonging to the class of N-azoles., i.e., a class of five-membered nitrogen heteroaromatic ring compounds containing at least one other atom of nitrogen. N-azoles, which are especially useful as secondary enhancers are imidazole, 1-methylimidazole, 1,2,3-triazole and 1,2,4-triazole. The invention also describes the use in diagnostic assays of chemiluminescent substrates containing said N-azoles, as secondary enhancers.