Abstract: A holographic microscopy characterization (HMC) process for utilizing holographic video microscopy to provide an efficient, automated, label-free method of accurately identifying cell viability. Optical properties of a sample of cells are determined by HMC. The optical properties are compared to known samples or compared over time to observe changes in the optical properties, enabling identification of cells as viable or not viable, or as extra-cellular or degraded cellular materials.

Abstract: An image decoding system provides a phase pattern encoding a target image. A spatial light modulator is configured to emit a wavefront-shaped light signal based on the phase pattern. A first diffusive medium receives the wavefront-shaped light signal and emits a decoded scattered light signal of the target image. The target image is previously encoded in the phase pattern by transmitting another wavefront-shaped light signal shaped by a training phase pattern through a second diffusive medium to yield a scattered light signal encoding a test image. The second diffusive medium has the optical scattering characteristics of a first diffusive medium. The scattered light signal encoding the test image is emitted from the second diffusive medium and recorded. The training phase pattern is adjusted for successive iterations of the test image until the test image satisfies a compensation condition. The resulting training phase pattern yields the phase pattern.

Abstract: An image decoding system provides a phase pattern encoding a target image. A spatial light modulator is configured to emit a wavefront-shaped light signal based on the phase pattern. A first diffusive medium receives the wavefront-shaped light signal and to emit a decoded scattered light signal of the target image. The target image is previously encoded in the phase pattern by transmitting another wavefront-shaped light signal shaped by a training phase pattern through a second diffusive medium to yield a scattered light signal encoding a test image. The second diffusive medium has the optical scattering characteristics of a first diffusive medium. The scattered light signal encoding the test image is emitted from the second diffusive medium and recorded. The training phase pattern is adjusted for successive iterations of the test image until the test image satisfies a compensation condition. The resulting training phase pattern yields the phase pattern.