Abstract: Noncultured Wharton's Jelly stem cells and methods of their purification, storage and use are provided.

Abstract: A clip of the present application generally comprises a first clip member, a second clip member, and a retention feature. The first clip member and the second clip member are coupled together and capable of being placed in at least two positions. In the first position, the clip is capable of receiving a bag containing a fluid, and, in the second position, the bag is capable of being held between the first and second clip members. While the clip is holding the bag, two or more pockets are formed in the bag, and the fluid is generally restricted or prohibited from transferring from one pocket to another pocket while the clip is in this position. A retention feature retains the clip, which is holding a bag, to a centrifuge receptacle and allows the clip to be placed in a predetermined position on the centrifuge receptacle.

Abstract: A clip of the present application generally comprises a first clip member, a second clip member, and a retention feature. The first clip member and the second clip member are coupled together and capable of being placed in at least two positions. In the first position, the clip is capable of receiving a bag containing a fluid, and, in the second position, the bag is capable of being held between the first and second clip members. While the clip is holding the bag, two or more pockets are formed in the bag, and the fluid is generally restricted or prohibited from transferring from one pocket to another pocket while the clip is in this position. A retention feature retains the clip, which is holding a bag, to a centrifuge receptacle and allows the clip to be placed in a predetermined position on the centrifuge receptacle.

Abstract: Noncultured Wharton's Jelly stem cells and methods of their purification, storage and use are provided.

Abstract: A clip of the present application generally comprises a first clip member, a second clip member, and a retention feature. The first clip member and the second clip member are coupled together and capable of being placed in at least two positions. In the first position, the clip is capable of receiving a bag containing a fluid, and, in the second position, the bag is capable of being held between the first and second clip members. While the clip is holding the bag, two or more pockets are formed in the bag, and the fluid is generally restricted or prohibited from transferring from one pocket to another pocket while the clip is in this position. A retention feature retains the clip, which is holding a bag, to a centrifuge receptacle and allows the clip to be placed in a predetermined position on the centrifuge receptacle.

Abstract: The present invention efficiently and cost-effectively extracts and collects cells from a tissue. The inventors have discovered that the tissue can be effectively fragmented and the resulting cells can be purified using a system or kit with multiple components. An advantage of the present invention is that tissue processing takes place in a closed system such that sterility can be maintained throughout the process, even if certain components are removed during processing, for example through the use of valves, clamps, and heat seals. Furthermore, any or all of the steps can be automated or manually accomplished, according to the specific needs of the application or the user.

Abstract: Noncultured Wharton's Jelly stem cells and methods of their purification, storage and use are provided.

Abstract: Noncultured Wharton's Jelly stem cells and methods of their purification, storage and use are provided.

Abstract: A method of culturing mesenchymal stem cells is provided. The mesenchymal stem cells are cultured in a medium that includes a cell-depleted Wharton's Jelly filtrate. The cell-depleted Wharton's Jelly filtrate is prepared by mincing umbilical cord tissue that includes Wharton's Jelly, subsequently diluting the umbilical cord tissue, subsequently filtering the umbilical cord tissue to generate a filtrate, and sedimenting Wharton's Jelly stem cells from the filtrate to generate the cell-depleted Wharton's Jelly filtrate.

Abstract: The present invention efficiently and cost-effectively extracts and collects cells from a tissue. The inventors have discovered that the tissue can be effectively fragmented and the resulting cells can be purified using a system or kit with multiple components. An advantage of the present invention is that tissue processing takes place in a closed system such that sterility can be maintained throughout the process, even if certain components are removed during processing, for example through the use of valves, clamps, and heat seals. Furthermore, any or all of the steps can be automated or manually accomplished, according to the specific needs of the application or the user.

Abstract: A clip of the present application generally comprises a first clip member, a second clip member, and a retention feature. The first clip member and the second clip member are coupled together and capable of being placed in at least two positions. In the first position, the clip is capable of receiving a bag containing a fluid, and, in the second position, the bag is capable of being held between the first and second clip members. While the clip is holding the bag, two or more pockets are formed in the bag, and the fluid is generally restricted or prohibited from transferring from one pocket to another pocket while the clip is in this position. A retention feature retains the clip, which is holding a bag, to a centrifuge receptacle and allows the clip to be placed in a predetermined position on the centrifuge receptacle.

Abstract: The present invention efficiently and cost-effectively extracts and collects cells from a tissue. The inventors have discovered that the tissue can be effectively fragmented and the resulting cells can be purified using a system or kit with multiple components. An advantage of the present invention is that tissue processing takes place in a closed system such that sterility can be maintained throughout the process, even if certain components are removed during processing, for example through the use of valves, clamps, and heat seals. Furthermore, any or all of the steps can be automated or manually accomplished, according to the specific needs of the application or the user.

Abstract: Noncultured Wharton's Jelly stem cells and methods of their purification, storage and use are provided.

Abstract: The present invention efficiently and cost-effectively extracts and collects cells from a tissue. The inventors have discovered that the tissue can be effectively fragmented and the resulting cells can be purified using a system or kit with multiple components. An advantage of the present invention is that tissue processing takes place in a closed system such that sterility can be maintained throughout the process, even if certain components are removed during processing, for example through the use of valves, clamps, and heat seals. Furthermore, any or all of the steps can be automated or manually accomplished, according to the specific needs of the application or the user.

Abstract: Noncultured Wharton's Jelly stem cells and methods of their purification, storage and use are provided.

Abstract: The present invention efficiently and cost-effectively extracts and collects cells from a tissue. The inventors have discovered that the tissue can be effectively fragmented and the resulting cells can be purified using a system or kit with multiple components. An advantage of the present invention is that tissue processing takes place in a closed system such that sterility can be maintained throughout the process, even if certain components are removed during processing, for example through the use of valves, clamps, and heat seals. Furthermore, any or all of the steps can be automated or manually accomplished, according to the specific needs of the application or the user.

Abstract: The present invention efficiently and cost-effectively extracts and collects cells from a tissue. The inventors have discovered that the tissue can be effectively fragmented and the resulting cells can be purified using a system or kit with multiple components. An advantage of the present invention is that tissue processing takes place in a closed system such that sterility can be maintained throughout the process, even if certain components are removed during processing, for example through the use of valves, clamps, and heat seals. Furthermore, any or all of the steps can be automated or manually accomplished, according to the specific needs of the application or the user.

Abstract: The present invention efficiently and cost-effectively extracts and collects cells from a tissue. The inventors have discovered that the tissue can be effectively fragmented and the resulting cells can be purified using a system or kit with multiple components. An advantage of the present invention is that tissue processing takes place in a closed system such that sterility can be maintained throughout the process, even if certain components are removed during processing, for example through the use of valves, clamps, and heat seals. Furthermore, any or all of the steps can be automated or manually accomplished, according to the specific needs of the application or the user.

Abstract: The present invention efficiently and cost-effectively extracts and collects cells from a tissue. The inventors have discovered that the tissue can be effectively fragmented and the resulting cells can be purified using a system or kit with multiple components. An advantage of the present invention is that tissue processing takes place in a closed system such that sterility can be maintained throughout the process, even if certain components are removed during processing, for example through the use of valves, clamps, and heal seals. Furthermore, any or all of the steps can be automated or manually accomplished, according to the specific needs of the application or the user.