Abstract: Provided is a method for synthesizing a chiral amine compound. A transaminase is used to transaminate a ketone substrate under the action of an amino donor, to obtain the chiral amine compound; and the conserved amino acid sequence region of the transaminase at least includes a region 1 (MAGLWCVN) and a region 2 (YNTFFKT). With the transaminase with the specific conserved amino acid sequence region to synthesize a large sterically hindered chiral amine, the enzyme catalytic reaction volume is small, the synthesizing route is short, the product yield is high, a high-cost noble metal is not required for catalysis under the synthesizing conditions, three wastes are reduced, and the production cost is saved.

Abstract: A method and system for recommending content using a chatbot are provided. The content recommendation method includes calling a chatbot to a chatroom based on a first user interaction with the chatroom; providing first content corresponding to a second user interaction with the chatroom while the chatbot is in the chatroom; and recommending second content related to the first content.

Abstract: The present invention relates to a series of neutral fluorescent mitochondrial markers as amide derivatives, and a preparation method and use thereof. The present invention provides for the first time that neutral dyes have excellent mitochondria targeting ability after being bonded to an amide compound. The present invention solves the problems of uncertain organelle targeting ability of the existing fluorescent dyes with a neutral structure and that the neutral dye is a commercial marker for lipid droplets in cells. In the present invention, the organelle targeting ability of a fluorescent dye is regulated by creatively modifying the structure, while the optical performance of the original dye is improved; and the biological properties of the dye are obviously improved after being bonded to an amide compound. Amide compounds are cheap and readily available, which is conducive to controlling the cost of new dyes, thus having great scientific significance and commercial value.

Abstract: The present disclosure relates to an electronic device, a method, and a computer-readable recording medium for electronic transactions. The electronic device displays a first graphical user interface (GUI) on a touch screen, the first GUI including a currency amount, displays a second GUI corresponding to transmission of the currency amount according to a currency transmission signal input to the touch screen, determines an amount of an electronic transaction according to the currency transmission signal, and transmits a generated signal containing information on the amount of the electronic transaction to an information processing system.

Abstract: The present disclosure provides a modified polyvinyl alcohol-based polymer, which comprises a modifying monomer unit represented by Formula wherein, X is a moiety comprising an alkenyl group, and the modified polyvinyl alcohol-based polymer has a saponification degree of 67 mol % to 78 mol % and a modification rate of 0.02 mol % to 1.5 mol %. The modified polyvinyl alcohol-based polymer is suitable to be used as a dispersant for suspension polymerization, to make the obtained polyvinyl chloride-based resin have reduced particle diameter and few coarse particles.

Abstract: The present invention provides a neutral fluorescent mitochondrial marker based on a nitrogen-containing heterocycle, and a preparation method and use thereof. The fluorophore in present invention is a heterocycle having a N—H bond and targeting mitochondria, which solves the problem that the ability of a fluorescent dye with a neutral structure to target organelles is random and uncertain, and also avoids the problem that the neutral fluorophore is a commercial marker for lipid droplets in cells. In the present invention, the organelle targeting ability of an original fluorophore is regulated by creatively modifying its structure while the optical performance of the fluorophore is improved. The marker improves the biological properties of the fluorophore, and the nitrogen-containing heterocycle building block is cheap and readily available, which is beneficial to controlling the cost of the new dye. The present invention has great scientific significance and commercial value.

Abstract: The present disclosure provides a modified polyvinyl alcohol-based polymer, which comprises a modifying monomer unit represented by Formula wherein, X is a moiety comprising an alkenyl group, and the modified polyvinyl alcohol-based polymer has a saponification degree of 67 mol % to 78 mol % and a modification rate of 0.02 mol % to 1.5 mol %. The modified polyvinyl alcohol-based polymer is suitable to be used as a dispersant for suspension polymerization, to make the obtained polyvinyl chloride-based resin have reduced particle diameter and few coarse particles.

Abstract: A method and system for recommending content using a chatbot are provided. The content recommendation method includes calling a chatbot to a chatroom based on a first user interaction with the chatroom; providing first content corresponding to a second user interaction with the chatroom while the chatbot is in the chatroom; and recommending second content related to the first content.

Abstract: The present disclosure relates to an electronic device, a method, and a computer-readable recording medium for electronic transactions. The electronic device displays a first graphical user interface (GUI) on a touch screen, the first GUI including a currency amount, displays a second GUI corresponding to transmission of the currency amount according to a currency transmission signal input to the touch screen, determines an amount of an electronic transaction according to the currency transmission signal, and transmits a generated signal containing information on the amount of the electronic transaction to an information processing system.

Abstract: A side mold working mechanism includes a cavity plate defining a mounting groove forming a receiving hole. Bilateral sides of the mounting groove hollow toward bilateral sides of the cavity plate to form two hollow grooves. Two guide blocks disposed in the hollow grooves have a leading track. The guide block defines a locating recess communicating with the leading track. Two locating blocks fixedly mounted in the locating recesses have a preventing portion placed on the leading track. A slide is movably assembled in the mounting groove. Bilateral sides of the top of the slide protrude outward to form two shoulders slidably supported by the leading tracks and capable of being blocked by the preventing portions. The top of the slide defines an inclined groove and an angular pin has an inclined bottom end for being inserted into or withdrawn out of the inclined groove from the receiving hole.

Abstract: A white-light light emitting diode (LED) and a method for regulating the generated light color of the LED, wherein the LED has at least one InGaN chip and at least one AlGaInP chip. When forward bias voltages are applied on the InGaN chip or AlGaInP chip, blue light and green-yellow light are generated and further mixed to create white light. By adjusting an external impedance value applied on the LED to regulate the forward bias voltages, pure white light is able to be derived based on the regulated brightness strength of the blue light and green-yellow light.

Abstract: Nucleic acids encoding each of three subunits, .alpha.1B, .alpha.2.delta., and .beta.3, of a calcium channel, are disclosed. Also disclosed are vectors containing the nucleic acids encoding the subunits; host cells containing the nucleic acids encoding the subunits; methods of isolating nucleic acids encoding related calcium channel subunits; the subunit proteins; fusion proteins comprising the subunit proteins; antibodies to the subunit proteins; assays to identify agents that modulate calcium channel activity, and agents identified thereby; methods of treating certain central nervous system disorders by altering calcium channel activity; and methods of diagnosing diseases associated with particular calcium channels, such as Lambert-Eaton syndrome.

Abstract: Nucleic acids encoding each of three subunits, .alpha.1B, .alpha.2.delta., and .beta.3, of a calcium channel, are disclosed. Also disclosed are vectors containing the nucleic acids encoding the subunits; host cells containing the nucleic acids encoding the subunits; methods of isolating nucleic acids encoding related calcium channel subunits; the subunit proteins; fusion proteins comprising the subunit proteins; antibodies to the subunit proteins; assays to identify agents that modulate calcium channel activity, and agents identified thereby; methods of treating certain central nervous system disorders by altering calcium channel activity; and methods of diagnosing diseases associated with particular calcium channels, such as Lambert-Eaton syndrome.