Abstract: A fluid filtration assembly includes a filter housing having a first end for fluid connection with a fluid storage vessel. A filter cartridge is disposable within the filter housing, and a plunger pump is coupled at a second end of the filter housing. The plunger pump includes a housing having a rigid portion and a flexible portion. The flexible portion has a plunger-engaging portion for coupling to the plunger of an actuator. The flexible portion selectively movable with respect to the rigid portion via the actuator. The filter cartridge can be a hollow fiber filter. The plunger pump can be configured to induce alternating tangential flow in at least a portion of the assembly. The fluid filtration assembly can be provided as a disposable single-use arrangement.

Abstract: A fluid filtration assembly includes a filter housing having first and second ends and a connector for fluid communication with a fluid storage vessel. A filter element is disposable within the filter housing, and first and second pumps are coupled at the first and second ends of the filter housing. A controller may coordinate the operation of the first and second pumps to induce alternating tangential flow of fluid between the filter housing and the first and second pumps. At least one of the first and second pumps is a diaphragm pump or a plunger pump. The fluid storage vessel can be a bioreactor.

Abstract: A control unit includes a first connector configured to connect a proportional pressure regulator to a positive pressure supply and a second connector configured to connect the proportional pressure regulator to a negative pressure supply. The control unit further includes at least one sensor configured to detect an amount of air flow (volume per unit of time), positive or negative, within an air flow line connected to an output of the proportional pressure regulator, and a third connector configured to connect the air flow line to an air side of a diaphragm. Additionally, the control unit includes a controller programmed to control at least an opening and closing function of the proportional pressure regulator to attain a desired amount of air flow (volume per unit of time), positive or negative, within the air flow line.

Abstract: A fluid filtration assembly includes a filter housing having a first end for fluid connection with a fluid storage vessel. A filter cartridge is disposable within the filter housing, and a plunger pump is coupled at a second end of the filter housing. The plunger pump includes a housing having a rigid portion and a flexible portion. The flexible portion has a plunger-engaging portion for coupling to the plunger of an actuator. The flexible portion selectively movable with respect to the rigid portion via the actuator. The filter cartridge can be a hollow fiber filter. The plunger pump can be configured to induce alternating tangential flow in at least a portion a the assembly. The fluid filtration assembly can be provided as a disposable single-use arrangement.

Abstract: A fluid filtration assembly includes a filter housing having first and second ends and a connector for fluid communication with a fluid storage vessel. A filter element is disposable within the filter housing, and first and second pumps are coupled at the first and second ends of the filter housing. A controller may coordinate the operation of the first and second pumps to induce alternating tangential flow of fluid between the filter housing and the first and second pumps. At least one of the first and second pumps is a diaphragm pump or a plunger pump. The fluid storage vessel can be a bioreactor.

Abstract: A control unit includes a first connector configured to connect a proportional pressure regulator to a positive pressure supply and a second connector configured to connect the proportional pressure regulator to a negative pressure supply. The control unit further includes at least one sensor configured to detect an amount of air flow (volume per unit of time), positive or negative, within an air flow line connected to an output of the proportional pressure regulator, and a third connector configured to connect the air flow line to an air side of a diaphragm. Additionally, the control unit includes a controller programmed to control at least an opening and closing function of the proportional pressure regulator to attain a desired amount of air flow (volume per unit of time), positive or negative, within the air flow line.

Abstract: A mixing system includes a container having a support plate and a mixing assembly supported on the support plate. The mixing assembly includes a pliable enclosure containing a fluid and a mixing device. A portion of the mixing device extends from the pliable enclosure and is adapted to be detachably coupled to a drive mechanism. A first plate is detachably secured to the rigid container. The pliable enclosure is disposed between the first plate and the support plate. A mixing arrangement includes a docking station having a drive cradle and a drive mechanism. The mixing system is removably positioned within the drive cradle so that the drive mechanism is removably coupled to the mixing device.

Abstract: A bag assembly includes a first panel secured to a second panel by a seam so that a cavity is formed between the first panel and the second panel, the seam encircling the cavity. A first seal couples together the first panel and the second panel at a location spaced apart from the seam so that the cavity encircles the first seal. In one embodiment, the first seal can be circular. A first port communicates with the cavity.

Abstract: A mixing system includes a container having a support plate and a mixing assembly supported on the support plate. The mixing assembly includes a pliable enclosure containing a fluid and a mixing device. A portion of the mixing device extends from the pliable enclosure and is adapted to be detachably coupled to a drive mechanism. A first plate is detachably secured to the rigid container. The pliable enclosure is disposed between the first plate and the support plate. A mixing arrangement includes a docking station having a drive cradle and a drive mechanism. The mixing system is removably positioned within the drive cradle so that the drive mechanism is removably coupled to the mixing device.

Abstract: A single-use fluid storage and filtration system includes a process vessel, a filter module including a hollow fiber filter element, and a drive module coupled to the filter module. The filter module is fixed to the process vessel and is in fluid communication with the process vessel for filtering a fluid received from the process vessel. The drive module includes a pump to induce flow of the fluid between the filter module and the process vessel. The process vessel, filter module and drive module comprise a single integrated and sterilized assembly.

Abstract: A mixing system includes a tank assembly and a container positioned within the tank assembly, the container bounding a compartment. A mixer is disposed within the compartment of the container. A shaft has a first end secured to the mixer and an opposing second end disposed outside of the container. An actuation mechanism is coupled with the second end of the shaft outside of the container and reciprocally moves the shaft and the mixer a stroke length, the stroke length being adjusted based on a pre-programmed protocol or signals from a sensor.

Abstract: A fluid filtration assembly includes a filter housing having a first end for fluid connection with a fluid storage vessel. A filter cartridge is disposable within the filter housing, and a plunger pump is coupled at a second end of the filter housing. The plunger pump includes a housing having a rigid portion and a flexible portion. The flexible portion has a plunger-engaging portion for coupling to the plunger of an actuator. The flexible portion selectively movable with respect to the rigid portion via the actuator. The filter cartridge can be a hollow fiber filter. The plunger pump can be configured to induce alternating tangential flow in at least a portion a the assembly. The fluid filtration assembly can be provided as a disposable single-use arrangement.

Abstract: A fluid filtration assembly includes a filter housing having first and second ends and a connector for fluid communication with a fluid storage vessel. A filter element is disposable within the filter housing, and first and second pumps are coupled at the first and second ends of the filter housing. A controller may coordinate the operation of the first and second pumps to induce alternating tangential flow of fluid between the filter housing and the first and second pumps. At least one of the first and second pumps is a diaphragm pump or a plunger pump. The fluid storage vessel can be a bioreactor.

Abstract: A bag assembly includes a first panel secured to a second panel by a seam so that a cavity is formed between the first panel and the second panel, the seam encircling the cavity. A first seal couples together the first panel and the second panel at a location spaced apart from the seam so that the cavity encircles the first seal. In one embodiment, the first seal can be circular. A first port communicates with the cavity.

Abstract: A control unit includes a first connector configured to connect a proportional pressure regulator to a positive pressure supply and a second connector configured to connect the proportional pressure regulator to a negative pressure supply. The control unit further includes at least one sensor configured to detect an amount of air flow (volume per unit of time), positive or negative, within an air flow line connected to an output of the proportional pressure regulator, and a third connector configured to connect the air flow line to an air side of a diaphragm. Additionally, the control unit includes a controller programmed to control at least an opening and closing function of the proportional pressure regulator to attain a desired amount of air flow (volume per unit of time), positive or negative, within the air flow line.

Abstract: An aseptic connector assembly includes a body portion having a receptacle configured to receive a corresponding fill connector. A pivot portion is movably coupled to the body portion, the pivot portion having a channel formed therein. A conduit portion is slidably disposed within the channel formed in the pivot portion. The pivot portion has a ready position and an operative position, the pivot portion being adjustable from the ready position to the operative position via relative movement with respect to the body portion.

Abstract: A fluid filtration assembly includes a filter housing having a first end for fluid connection with a fluid storage vessel. A filter cartridge is disposable within the filter housing, and a plunger pump is coupled at a second end of the filter housing. The plunger pump includes a housing having a rigid portion and a flexible portion. The flexible portion has a plunger-engaging portion for coupling to the plunger of an actuator. The flexible portion selectively movable with respect to the rigid portion via the actuator. The filter cartridge can be a hollow fiber filter. The plunger pump can be configured to induce alternating tangential flow in at least a portion of the assembly. The fluid filtration assembly can be provided as a disposable single-use arrangement.

Abstract: A bag assembly includes a pliable housing having a first panel secured to a second panel by a seam so that a cavity is formed between the first panel and the second panel, the seam encircling the cavity. A first tufting couples together the first panel and the second panel at a location spaced apart from the seam so that the cavity encircles the first tufting. In one embodiment the first tufting can circular. A first port is coupled with the pliable housing and communicating with the cavity.

Abstract: A method for forming an aseptic connection includes inserting an end of a sealed fill connector into a receptacle of an aseptic connector assembly; moving a pivot portion of the aseptic connector assembly relative to a body portion of the aseptic connector assembly so as to sever a portion of the fill connector, the pivot portion being at least partially disposed within the body portion; and advancing a conduit portion of the aseptic connector assembly into the fill connector so as to form an aseptic connection therebetween, the conduit portion being at least partially disposed within the pivot portion or the body portion of the aseptic connector assembly after advancing.

Abstract: A bag assembly includes a pliable housing having a first panel secured to a second panel by a seam so that a cavity is formed between the first panel and the second panel, the seam encircling the cavity. A first tufting couples together the first panel and the second panel at a location spaced apart from the seam so that the cavity encircles the first tufting. In one embodiment the first tufting can circular. A first port is coupled with the pliable housing and communicating with the cavity.