Patents by Inventor Rudolf Seibl
Rudolf Seibl has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 8357490Abstract: An integrated instrument for oligonucleotide synthesis and PCR, and a system and method thereof are disclosed. The integrated instrument is basically composed of two independent modules. The first module is a unit for chemical de novo synthesis of oligonucleotides such as oligonucleotide primers and/or oligonucleotide hybridization probes. The second module is a unit for performing an analytical polymerase chain reaction amplification in real time, i.e. a qPCR. The two modules are operatively linked to each other in such a way that a user can load a nucleic sample to be analyzed into the integrated instrument and perform a PCR reaction by programming the instrument without a previous external synthesis of oligonucleotide amplification primers.Type: GrantFiled: July 22, 2010Date of Patent: January 22, 2013Assignee: Roche Diagnostics Operations, Inc.Inventors: Thomas Froehlich, Martin Gutekunst, Dieter Heindl, Angelika Roesler, Rudolf Seibl
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Publication number: 20110177514Abstract: An integrated instrument for oligonucleotide synthesis and PCR, and a system and method thereof are disclosed. The integrated instrument is basically composed of two independent modules. The first module is a unit for chemical de novo synthesis of oligonucleotides such as oligonucleotide primers and/or oligonucleotide hybridization probes. The second module is a unit for performing an analytical polymerase chain reaction amplification in real time, i.e. a qPCR. The two modules are operatively linked to each other in such a way that a user can load a nucleic sample to be analyzed into the integrated instrument and perform a PCR reaction by programming the instrument without a previous external synthesis of oligonucleotide amplification primers.Type: ApplicationFiled: July 22, 2010Publication date: July 21, 2011Applicant: Roche Diagnostics Operations, Inc.Inventors: Thomas Froehlich, Martin Gutekunst, Dieter Heindl, Angelika Roesler, Rudolf Seibl
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Patent number: 6001610Abstract: A method of detecting a target nucleic acid A is disclosed, comprising hybridizing the target nucleic acid A with a probe nucleic acid B which contains a sequence B1 which base pairs with a part of the target nucleic acid A and a sequence B2, cleaving the hybridized probe nucleic acid B to produce a cleavage product B' containing the sequence B2, hybridizing the cleavage product B' with a template nucleic acid C containing a sequence C2 which base pairs with a part of the cleavage product B' and a sequence C1 which does not hybridize with the sequence B1 of the probe nucleic acid B, extending the hybridized cleavage product B' with an extension sequence B3 which is template-specific to a part of the sequence C1, hybridizing a probe D with the extension product, wherein the probe D contains a sequence D1 which base pairs with the extension sequence B3 and a sequence D2, and detecting any of the various products formed throughout the method. Products for performing the method are also disclosed.Type: GrantFiled: February 9, 1998Date of Patent: December 14, 1999Assignee: Roche Diagnostics, GmbHInventors: Rudolf Seibl, Viola Rosemeyer
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Patent number: 5989812Abstract: An improved method of transcribing nucleic acids wherein the amount of abortive transcripts formed is reduced wherein the improvement comprises an effector sequence in the transcription reaction.Type: GrantFiled: November 22, 1995Date of Patent: November 23, 1999Assignee: Boehringer Mannheim GmbHInventors: Viola Rosemeyer, Rudolf Seibl
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Patent number: 5830664Abstract: Method for the sensitive detection of a target nucleic acid by hybridization with a probe nucleic acid. The latter contains a part which can hybridize with the target nucleic acid and a nucleic acid-specific part which does not hybridize with the target nucleic acid. The method further comprises cleavage of the probe nucleic acid, hybridization of a cleavage product of the probe nucleic acid containing the part that does not hybridize with the target nucleic acid with a matrix nucleic acid containing a part that can be hybridized with the cleavage product and a part that cannot be hybridized with the probe nucleic acid. The method also comprises the determination of the hybrid consisting of the cleavage product and the matrix nucleic acid and a reagent kit suitable for this purpose.Type: GrantFiled: July 17, 1995Date of Patent: November 3, 1998Assignee: Boehringer Mannheim GmbHInventors: Viola Rosemeyer, Rudolf Seibl
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Patent number: 5783392Abstract: A method of detecting a target nucleic acid A is disclosed, comprising hybridizing the target nucleic acid A with a probe nucleic acid B which contains a sequence B1 which base pairs with a part of the target nucleic acid A and a sequence B2, cleaving the hybridized probe nucleic acid B to produce a cleavage product B' containing the sequence B2, hybridizing the cleavage product B' with a template nucleic acid C containing a sequence C2 which base pairs with a part of the cleavage product B' and a sequence C1 which does not hybridize with the sequence B1 of the probe nucleic acid B, extending the hybridized cleavage product B' with an extension sequence B3 which is template-specific to a part of the sequence C1, hybridizing a probe D with the extension product, wherein the probe D contains a sequence D1 which base pairs with the extension sequence B3 and a sequence D2, and detecting any of the various products formed throughout the method. Products for performing the method are also disclosed.Type: GrantFiled: November 22, 1995Date of Patent: July 21, 1998Assignee: Boehringer Mannheim GmbHInventors: Rudolf Seibl, Viola Rosemeyer
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Patent number: 5753433Abstract: Method for the specific detection of nucleic acids in a sample by reaction of the sample with one or several labelled nucleotide triphosphates and one or several enzymes which catalyse the production of a labelled nucleic acid B which contains this nucleotide, non-thermally denaturation, reacting the sample with a nucleic acid probe C which is sufficiently complementary to nucleic acid B and which contains at least one immobilizable group, if desired, contacting the nucleic acid hybrid D formed with a solid phase which recognizes and binds the immobilizable group, removing the liquid from the solid phase and determining the label on the solid phase as a measure for the presence of the nucleic acid.Type: GrantFiled: September 19, 1994Date of Patent: May 19, 1998Assignee: Boehringer Mannheim GmbHInventors: Christoph Kessler, Rudiger Ruger, Rudolf Seibl, Cornelia Kruse-Muller, Sibylle Berner
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Patent number: 5702888Abstract: For the detection of nucleic acids of definite sequence by hybridisation with a complementary nucleic acid probe which contains bound via a chemical bonding at least one hapten as labelling one uses, as hapten, a steroid which is bound vie a bridge of at least 4 atoms length to at least one position of the nucleic acid probe which does not participate in hydrogen bridge formation and detects the hybridised probe via an in turn labelled anti-hapten antibody.Type: GrantFiled: January 11, 1995Date of Patent: December 30, 1997Assignee: Boehringer Mannheim GmbHInventors: Hans-Joachim Holtke, Rudolf Seibl, Gudrun Schmitz, Hans Robert Scholer, Christoph Kessler, Ralf Mattes
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Patent number: 5635350Abstract: Method of determining polymerase activity by incubating the polymerase with a template nucleic acid, one detectable mononucleoside triphosphate and one immobilizable nucleoside triphosphate, binding the immobilizable nucleotides to a solid phase and detecting the bound delectable nucleotides and tests based thereon.Type: GrantFiled: February 3, 1995Date of Patent: June 3, 1997Assignee: Boehringer Mannheim GmbHInventors: Josef Eberle, Rudolf Seibl, Christoph Kessler, Bernhard Konig
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Patent number: 5573913Abstract: The present invention concerns a process for introducing non-radioactively labelled deoxynucleotides into nucleic acids and RNA molecules which at their 3' end contain at least one deoxynucleotide which carries a non-radioactive marker group.Type: GrantFiled: February 28, 1995Date of Patent: November 12, 1996Assignee: Boehringer Mannheim GmbHInventors: Viola Rosemeyer, Rudolf Seibl, Andreas Laubrock
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Patent number: 5413906Abstract: Method of determining polymerase activity by incubating the polymerase with a template nucleic acid, one detectable mononucleoside triphosphate and one immobilizable nucleoside triphosphate, binding the immobilizable nucleotides to a solid phase and detecting the bound detectable nucleotides and tests based thereon.Type: GrantFiled: March 19, 1993Date of Patent: May 9, 1995Assignee: Boehringer Mannheim GmbHInventors: Josef Eberle, Rudolf Seibl, Christoph Kessler, Bernhard Konig
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Patent number: 5369003Abstract: Process for the production of ribonucleic acids or for the detection of template nucleic acids based on a transcription technique using two oligonucleotides which can hybridize to adjacent regions on the template nucleic acid without ligation of these oligonucleotides.Type: GrantFiled: September 28, 1992Date of Patent: November 29, 1994Assignee: Boehringer Mannheim GmbHInventors: Udo Reischl, Rudiger Rueger, Christoph Kessler, Rudolf Seibl
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Patent number: 5354657Abstract: The present invention provides a process for the detection of nucleic acids of definite sequence by hybridisation with two single-stranded nucleic acid probes present in the same solution phase complementary to different regions of the nucleic acid to be detected, one nucleic acid probe serving as detector probe and containing as labelling at least one hapten bound via a chemical linkage and the other nucleic acid probe serving as capturing probe and being bound to a solid matrix, wherein, as hapten, a steroid is used which on at least one position of the detector probe, which does not participate in the hydrogen bridge formation with the nucleic acid to be detected, is covalently bound via a bridge of at least four atoms, the nucleic acid to be detected, present in solution, is incubated with the detector probe and the capturing probe in any desired sequence, whereby the binding of the capturing probe on the matrix with the nucleic acid to be detected is brought about before, during or after the incubation,Type: GrantFiled: December 18, 1992Date of Patent: October 11, 1994Inventors: Hans J. Holtke, Rudolf Seibl, Christoph Kessler, Ralf Mattes, Hermann Graf
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Patent number: 5344757Abstract: For the detection of nucleic acids of definite sequence by hybridisation with a complementary nucleic acid probe which contains bound via a chemical bonding at least one hapten as labelling one uses, as hapten, a steroid which is bound via a bridge of at least 4 atoms length to at least one position of the nucleic acid probe which does not participate in hydrogen bridge formation and detects the hybridised probe via an in turn labelled anti-hapten antibody.Type: GrantFiled: January 9, 1990Date of Patent: September 6, 1994Assignee: Boehringer Mannheim GmbHInventors: Hans-Joachim Holtke, Rudolf Seibl, Gudrun Schmitz, Hans R. Scholer, Christoph Kessler, Ralf Mattes