Abstract: The present invention discloses an microvascular anastomosis device. The device comprises two rotatable and movable ring bodies disposed relatively on a guiding base, and a guiding member lead two vessels to pass through the two ring bodies respectively, wherein a tenon structure is disposed at the two ring bodies for aligning and embedding each other, and inner surface of each vessel is pressured in axial symmetry to make the outer surface of each vessel adhered and fixed onto the inner surface of each ring body, so as not to turn the vessels inside out when performing microvascular Anastomsis.