Abstract: In a detection method, first dielectric particles each capable of being bound to a first target substance and second dielectric particles each capable of being bound to a second target substance are caused to react with a sample that contains a first target substance and a second target substance, the second dielectric particles having a different dielectrophoretic property from the first dielectric particles, a first composite particle to which the first target substance is bound is separated from the other first dielectric particle, and a second composite particle to which the second target substance is bound is separated from the other second dielectric particle by causing dielectrophoresis in the sample after the reaction, and the first target substance contained in the separated first composite particle and the second target substance contained in the second composite particle are each detected.

Abstract: A detection method is a detection method for detecting a target substance from among in-liquid substances including the target substance and a non-target substance, and includes (i) applying an alternating-current voltage to an electrode pair that generates an electric field gradient so that positive dielectrophoretic force is exerted on the target substance and the non-target substance; and (ii) applying an alternating-current voltage after execution of the (i) applying so that negative dielectrophoretic force is exerted on the target substance on which the positive dielectrophoretic force has been acting.

Abstract: A counting method includes aggregating particles in a sample by the action of first dielectrophoretic force, dispersing the aggregated particles by the action of second dielectrophoretic force, which is different from the first dielectrophoretic force, capturing a dispersion image including the dispersed particles, and determining the number of particles on the basis of the dispersion image.

Abstract: A counting method includes aggregating particles in a sample by action of first-direction dielectrophoretic force, dispersing the aggregated particles by action of second-direction dielectrophoretic force, which is different from the first-direction dielectrophoretic force, capturing a dispersion image including the dispersed particles, and determining the number of particles on the basis of the dispersion image.

Abstract: A detection method includes forming a complex by binding a target substance and a dielectric particle modified by a single-domain antibody that is bindable to the target substance, separating the complex and an unbound particle in a fluid with dielectrophoresis, the unbound particle being the dielectric particle not forming the complex, and detecting the target substance contained in the separated complex with an imaging element.

Abstract: There is provided a counting method for counting a moving bonded body by exerting an external force in a predetermined direction, on a sample capable of containing the bonded body in which a target substance and a moving substance moving in response to exertion of the external force are bonded, the counting method including: obtaining a captured image of an object in the sample captured in a period from a first time point to a second time point later than the first time point during which the external force is exerted; determining a moving direction of the object from a locus, in the image, formed by the object; and counting the object as the bonded body in a case where a degree of coincidence between the predetermined direction and the moving direction is greater than a first threshold.