Abstract: Beads with functionalized material applied to them are exposed to an acoustic field to trap, retain or pass the beads. The beads may include or be free of ferro magnetic material. The beads may be biocompatible or biodegradable for a host. The size of the beads may vary over a range, and/or be heterogenous or homogenous. The composition of the beads may include high, neutral or low acoustic contrast material. The chemistry of the functionalized material may be compatible with existing processes. The acoustic field may be generated, for example, in an acoustic angled wave device or in an acoustic fluidized bed.

Abstract: Acoustic forces in an acoustic field can be increased via introduction of “seeding particles” with higher or similar contrast factor and/or size relative to the particles targeted for retention in the acoustic field. This feature may be implemented in an acoustic concentration device or an acoustic separation device. Increases in acoustic forces lead to better particle retention and can permit increased flow rates through an acoustic particle processing device.

Abstract: Microparticles and nanoparticles made of various materials that are used in various configurations are disclosed. The particles may be perfluorocarbon droplets with a lipid coating. The particles may be used in an acoustic cell selection process. The droplets are highly acoustically responsive and can be retained against fluid flow by an acoustic field. Such particles can be used in the separation, segregation, differentiation, modification or filtration of a system.

Abstract: Separation of materials is achieved using affinity binding and acoustophoretic techniques. A column provided with a fluid mixture of materials for separation and support structures may be used with acoustic waves to block flow of the support structures. The support structures can have an affinity for one or more materials in the fluid mixture. By blocking flow of the support structures, materials bound or adhered to the support structure are also blocked.

Abstract: Separation of materials is achieved using affinity binding and acoustophoretic techniques. A column provided with a fluid mixture of materials for separation and support structures may be used with acoustic waves to block flow of the support structures. The support structures can have an affinity for one or more materials in the fluid mixture. By blocking flow of the support structures, materials bound or adhered to the support structure are also blocked.

Abstract: Separation of materials is achieved using affinity binding and acoustophoretic techniques. A column provided with a fluid mixture of materials for separation and support structures may be used with acoustic waves to block flow of the support structures. The support structures can have an affinity for one or more materials in the fluid mixture. By blocking flow of the support structures, materials bound or adhered to the support structure are also blocked.

Abstract: A closed and modular fluidic system composed of one or more acoustic elements and cell processing reagents. The system employs a cellular manufacturing process for producing cell and gene therapy therapeutics.

Abstract: A closed and modular fluidic system composed of one or more acoustic elements and cell processing reagents. The system employs a cellular manufacturing process for producing cell and gene therapy therapeutics.

Abstract: Separation of materials is achieved using affinity binding and acoustophoretic techniques. A column provided with a fluid mixture of materials for separation and support structures may be used with acoustic waves to block flow of the support structures. The support structures can have an affinity for one or more materials in the fluid mixture. By blocking flow of the support structures, materials bound or adhered to the support structure are also blocked.

Abstract: Beads with functionalized material applied to them are exposed to an acoustic field to trap, retain or pass the beads. The beads may include or be free of ferro magnetic material. The beads may be biocompatible or biodegradable for a host. The size of the beads may vary over a range, and/or be heterogenous or homogenous. The composition of the beads may include high, neutral or low acoustic contrast material. The chemistry of the functionalized material may be compatible with existing processes. The acoustic field may be generated, for example, in an acoustic angled wave device or in an acoustic fluidized bed.

Abstract: A closed and modular fluidic system composed of one or more acoustic elements and cell processing reagents. The system employs a cellular manufacturing process for producing cell and gene therapy therapeutics.

Abstract: A bioreactor utilizing a multilayer disposable bag that may include at least one ultrasonic transducer that can acoustically generate a multi-dimensional standing wave. The standing wave can be used to retain cells in the bioreactor, and can also be utilized to dewater or further harvest product from the waste materials produced in a bioreactor.

Abstract: Methods for introducing foreign nucleic acids into cells, such as by performing transfection/transduction, using acoustic processes are disclosed herein. The foreign DNA/RNA and the cells are co-located in a multi-dimensional acoustic standing wave, or are co-located by acoustic streaming.

Abstract: Microparticles and nanoparticles made of various materials that are used in various configurations are disclosed. Such particles can also contain various types of materials as payloads to be used in the separation, segregation, differentiation, modification or filtration of a system or a host anatomy. The microparticles and nanoparticles are utilized in conjunction with an acoustic standing wave or an acoustic traveling wave in various processes.

Abstract: Separation of materials is achieved using affinity binding and acoustophoretic techniques. A column provided with a fluid mixture of materials for separation and support structures may be used with acoustic waves to block flow of the support structures. The support structures can have an affinity for one or more materials in the fluid mixture. By blocking flow of the support structures, materials bound or adhered to the support structure are also blocked.

Abstract: Separation of materials is achieved using affinity binding and acoustophoretic techniques. A column provided with a fluid mixture of materials for separation and support structures may be used with acoustic waves to block flow of the support structures. The support structures can have an affinity for one or more materials in the fluid mixture. By blocking flow of the support structures, materials bound or adhered to the support structure are also blocked.

Abstract: A closed and modular fluidic system composed of one or more acoustic elements and cell processing reagents. The system employs a cellular manufacturing process for producing cell and gene therapy therapeutics.

Abstract: Separation of materials is achieved using affinity binding and acoustophoretic techniques. A column provided with a fluid mixture of materials for separation and support structures may be used with acoustic waves to block flow of the support structures. The support structures can have an affinity for one or more materials in the fluid mixture. By blocking flow of the support structures, materials bound or adhered to the support structure are also blocked.

Abstract: Described herein are microreactors and methods of modifying support particles using acoustic standing waves. A liquid medium containing suspended support particles is flowed along a flow path through a channel, and an acoustic standing wave is applied to the channel to hold the suspended particles at a point in the channel. The held particles are then subjected to one or more reactions by flowing biochemical reactants through the channel. Unbound biological reactant is optionally washed from the channel. The reacted support particles can be released from the acoustic standing wave for further processing using, for example, flow cytometry or fluorescence microscopy.

Abstract: Methods for causing interaction between two sets of particles are disclosed herein. The two sets of particles are co-located in a multi-dimensional acoustic standing wave, or are co-located by acoustic streaming. This is more effective than conventional methods, for example by increasing the homogeneity of the fluid mixture containing the particles, while using reduced amounts of one particle set.