Abstract: A method and system for detecting Staphylococcus aureus in chicken is provided. The method includes: obtaining hyperspectral images of samples; selecting spectral images at characteristic wavelengths based on the hyperspectral images of the samples, setting grayscale thresholds, and segmenting the selected spectral images to obtain a chicken sample region to be detected and a Staphylococcus aureus region to be detected; extracting hyperspectral data; extracting characteristic wavelengths after mixing the hyperspectral data of the chicken samples with the hyperspectral data of Staphylococcus aureus; selecting the hyperspectral data of the chicken samples corresponding to the extracted characteristic wavelengths to train a support vector machine model, thereby obtaining a detection model for Staphylococcus aureus; and detecting Staphylococcus aureus in chicken by using the detection model for Staphylococcus aureus.