Patents by Inventor Ruina He
Ruina He has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11577218Abstract: Provided is a high-loading and alkali-resistant protein A magnetic bead. The magnetic bead can maintain chemical stability under pH 2-14 and has an immunoglobulin G (IgG) binding capacity greater than 50 mg/mL. Further provided is a method for purifying and/or detecting an immunoglobulin, comprising a step of contacting a sample containing the immunoglobulin with the high-loading and alkali-resistant protein A magnetic bead. The alkali-resistant protein A magnetic bead can realize rapid purification of immunoglobulin, saving about 80% of treatment time and reducing total purification costs by 50%. In addition, the alkali-resistant protein A magnetic bead has high alkali resistance. An alkaline method for in situ cleaning can be performed to regenerate the magnetic bead after use. The magnetic bead has rapid magnetic response and good dispersiveness, realizing rapid magnetic bead enrichment, cleaning, and elution.Type: GrantFiled: January 4, 2018Date of Patent: February 14, 2023Assignee: Nanjing GenScript Biotech Co., Ltd.Inventors: Chao Wang, Ruina He, Weijuan Han, Hong Qian, Tao Bai
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Patent number: 11260400Abstract: An inclined magnetic holder comprises a magnetic base and a centrifuge tube support plate. The centrifuge tube support plate has centrifuge tube support holes. The magnetic base comprises a first bottom plate, a fixing plate, and two first-side support plates. Respective top portions of the two first-side support plates are provided with a position-locating slot. Two ends of the centrifuge tube support plate are respectively provided with a position-locating protruding block. The centrifuge tube support holes are evenly and linearly distributed on the centrifuge tube support plate. An elastic circular engagement component for holding a centrifuge tube is provided inside the centrifuge tube support holes. A block magnet is fixed to the fixing plate below and corresponding to each of the centrifuge tube support holes. A north pole or south pole surface of the block magnet faces the centrifuge tube and is parallel to an axis of the centrifuge tube.Type: GrantFiled: October 30, 2018Date of Patent: March 1, 2022Assignee: Nanjing GenScript Biotech Co., Ltd.Inventors: Jinxin Zhu, Hong Qian, Weijuan Han, Ruina He, Tao Bai, Chao Wang
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Patent number: 11254906Abstract: An automatic purification system using magnetic particles comprises: 1) a first container module; and 2) a system controller module. The first container module comprises a first container and a first magnetic field supply device disposed outside the first container. A first container liquid inlet is formed in the upper portion of the first container, and a first container liquid outlet is formed in the bottom of the first container. The system controller module can generate a variable magnetic field in the first container by controlling the first magnetic field supply device. A method for purifying a target component from a biological sample comprises a step for allowing a biological sample containing a target component to be in contact with magnetic particles capable of specifically binding the target component, in the first container in the automatic purification system.Type: GrantFiled: January 4, 2018Date of Patent: February 22, 2022Assignee: Nanjing GenScript Biotech Co., Ltd.Inventors: Xin Chen, Ruina He, Chao Wang, Hong Qian, Tao Bai
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Publication number: 20210388307Abstract: The present invention relates to a large-scale magnetic purification system, including: a liquid storage apparatus, capable of being connected to a liquid source and a waste liquid barrel through a liquid path system; a stirring system mounted above the liquid storage apparatus, where a stirring paddle of the stirring system is inserted into the liquid storage apparatus for stirring; magnets mounted around the liquid storage apparatus; a magnet actuating mechanism for manipulating the magnets to move away from or close to the liquid storage apparatus; and a control system, connected to the liquid path system, the magnet actuating mechanism, and the stirring system and controlling the liquid path system, the magnet actuating mechanism, and the stirring system.Type: ApplicationFiled: October 25, 2019Publication date: December 16, 2021Applicant: Nanjing GenScript Biotech Co., Ltd.Inventors: Guodong CHEN, Zaidong FENG, Ruina HE, Hong QIAN, Tao BAI
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Publication number: 20210299677Abstract: The present invention provides a magnetic bead purification system, including: a housing; a liquid path treatment system provided inside the housing, the liquid path treatment system being connectable to a reagent barrel and a waste liquid barrel; a sample addition needle group connected to the liquid path treatment system, the sample addition needle group being movable within the housing and connected to the liquid path treatment system, so as to receive a reagent from the liquid path treatment system or to discharge a waste liquid to the liquid path treatment system; a purification magnetic separation system, including a magnetic element, the purification magnetic separation system being controllable to apply a lateral magnetic force to a purification treatment position inside the housing or stop the application of the magnetic force by the magnetic element; and a purification station system movable between a purification treatment position inside the housing and a loading position outside the housing, theType: ApplicationFiled: April 26, 2019Publication date: September 30, 2021Applicant: Nanjing GenScript Biotech Co., Ltd.Inventors: Jinxin Zhu, Ruina He, Hong Qian, Tao Bai, Deming Li, Cheng Zheng, Guodong Chen
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Publication number: 20210069726Abstract: An inclined magnetic holder is disclosed, comprising a magnetic base and a centrifuge tube support plate. The centrifuge tube support plate is provided with centrifuge tube support holes. The magnetic base comprises a first bottom plate, a fixing plate provided on the first bottom plate, and two first-side support plates respectively provided at two sides of the fixing plate. Respective top portions of the two first-side support plates are provided with a position-locating slot. A bottom surface of the position-locating slot is configured with an inclined angle. Two ends of the centrifuge tube support plate are respectively provided with a position-locating protruding block for fitting and assembling into the position-locating slot. The centrifuge tube support holes are evenly and linearly distributed on the centrifuge tube support plate. An elastic circular engagement component for holding a centrifuge tube is provided inside the centrifuge tube support holes.Type: ApplicationFiled: October 30, 2018Publication date: March 11, 2021Applicant: Nanjingjinsirui Science & Technology Biology Corp.Inventors: Jinxin Zhu, Hong Qian, Weijuan Han, Ruina He, Tao Bai, Chao Wang
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Publication number: 20190376022Abstract: An automatic purification system using magnetic particles comprises: 1) a first container module; and 2) a system controller module. The first container module comprises a first container and a first magnetic field supply device disposed outside the first container. A first container liquid inlet is formed in the upper portion of the first container, and a first container liquid outlet is formed in the bottom of the first container. The system controller module can generate a variable magnetic field in the first container by controlling the first magnetic field supply device. A method for purifying a target component from a biological sample comprises a step for allowing a biological sample containing a target component to be in contact with magnetic particles capable of specifically binding the target component, in the first container in the automatic purification system.Type: ApplicationFiled: January 4, 2018Publication date: December 12, 2019Applicant: Nanjingjinsirui Science & Technology Biology Corp.Inventors: Xin Chen, Ruina He, Chao Wang, Hong Qian, Tao Bai
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Publication number: 20190329218Abstract: Provided is a high-loading and alkali-resistant protein A magnetic bead. The magnetic bead can maintain chemical stability under pH 2-14 and has an immunoglobulin G (IgG) binding capacity greater than 50 mg/mL. Further provided is a method for purifying and/or detecting an immunoglobulin, comprising a step of contacting a sample containing the immunoglobulin with the high-loading and alkali-resistant protein A magnetic bead. The alkali-resistant protein A magnetic bead can realize rapid purification of immunoglobulin, saving about 80% of treatment time and reducing total purification costs by 50%. In addition, the alkali-resistant protein A magnetic bead has high alkali resistance. An alkaline method for in situ cleaning can be performed to regenerate the magnetic bead after use. The magnetic bead has rapid magnetic response and good dispersiveness, realizing rapid magnetic bead enrichment, cleaning, and elution.Type: ApplicationFiled: January 4, 2018Publication date: October 31, 2019Applicant: Nanjingjinsirui Science & Technology Biology Corp.Inventors: Chao Wang, Ruina He, Weijuan Han, Hong Qian, Tao Bai