Abstract: The present application relates to the technical field of information and relates to a sequencing method. The sequencing method provided by the present application includes the following steps: using a sequencing system to perform base extension reactions on nucleic acid samples; and outputting sequencing data to an external server at nodes according to preset numbers of base extension cycles, and outputting base calling results by the external server on the basis of the sequencing data. The sequencing method provided by the present application enables the transmission of sequencing intermediate files of the preset numbers of base extension cycles and, by means of the base calling data for the sequenced samples according to preset requirements, helping increase the efficiency of sequencing of nucleic acid samples.

Abstract: A device and method for enhancing nitrogen and phosphorus removal based on a multistage AO partial denitrification coupled with Anammox process in combination with a sludge hydrolytic acidification mixture belong to the technical field of active sludge method wastewater treatment. A system includes a water tank, a water pump, a biochemical reaction zone, a hydrolytic acidification tank and other devices.

Abstract: A display substrate includes a backplane, first light-emitting devices capable of emitting first color light, second light-emitting devices capable of emitting second color light that are all disposed on the backplane, a low-refractive-index intercalation layer disposed at a side, away from the backplane, of the first light-emitting devices and the second light-emitting devices, and an encapsulation layer. The encapsulation layer includes a first inorganic barrier layer, a first organic barrier layer and a second inorganic barrier layer that are sequentially stacked on a side of the low-refractive-index intercalation layer away from the backplane. A refractive index of the low-refractive-index intercalation layer is less than a refractive index of the first inorganic barrier layer.

Abstract: A display substrate includes a backplane, first light-emitting devices capable of emitting first color light, second light-emitting devices capable of emitting second color light that are all disposed on the backplane, a low-refractive-index intercalation layer disposed at a side, away from the backplane, of the first light-emitting devices and the second light-emitting devices, and an encapsulation layer. The encapsulation layer includes a first inorganic barrier layer, a first organic barrier layer and a second inorganic barrier layer that are sequentially stacked on a side of the low-refractive-index intercalation layer away from the backplane. A refractive index of the low-refractive-index intercalation layer is less than a refractive index of the first inorganic barrier layer.