Abstract: The present disclosure provides a heat-sealing barrier cellulose film, and a preparation method and use thereof. In the present disclosure, the heat-sealing barrier cellulose film includes a separation layer, a regenerated cellulose support layer, and an adhesive layer that are laminated in sequence. The separation layer is prepared from a barrier coating (including a polyvinylidene chloride (PVDC) emulsion, a first anti-sticking agent, and water); and the adhesive layer is prepared from an adhesive coating (an emulsion (including an acrylate emulsion and/or a polyurethane emulsion), a second anti-sticking agent, and water). The heat-sealing barrier cellulose film has high adhesion and barrier properties, can be directly heat-pressed with paper products, and shows a high adhesion strength with the paper products, thus meeting the requirements of bonding strength in coated paper.