Abstract: The disclosure provides a method of preconditioning a subject for chimeric antigen receptor (CAR) T cell therapy. The method comprises administering to the subject a composition comprising a nanoparticle comprising a positively-charged surface and an interior comprising (i) a core and (ii) at least two nucleic acid layers, wherein each nucleic acid layer is positioned between a cationic lipid bilayer, at least one day prior to administering CAR T cell therapy to the subject. The disclosure also provides a method of treating a solid tumor in a subject, the method comprising administering to a subject comprising a surface antigen negative solid tumor a first composition comprising the nanoparticle, wherein the nucleic acid within the nanoparticle encodes the surface antigen, and a second composition comprising a CAR T cell that targets the surface antigen.

Abstract: Glasses include a main body, a glasses leg, and a connecting device. The glasses leg is connected to an end portion of the main body through the connecting device in such a way that the glasses leg is rotatable. The connecting device includes: a first connecting member, a second connecting member, and an elastic member, where the first connecting member is arranged on the end portion of the main body, a first end of the second connecting member is hinged with the first connecting member, a second end of the second connecting member is hinged with the glasses leg, and the elastic member is located between the second connecting member and the glasses leg, where a first elastic end of the elastic member abuts against the glasses leg, a second elastic end of the elastic member abuts against the second connecting member.

Abstract: A waveguide rod for an ultrasonic scalpel having a relatively desirable amplitude and frequency comprises a proximal gain structure, a distal gain structure, an intermediate structure, and a frequency adjustment structure. The proximal gain structure and the intermediate structure are connected in a position near an antinode of longitudinal vibration of the waveguide rod through a proximal side gain step. The distal gain structure and the intermediate structure are connected in a position near an antinode of the longitudinal vibration of the waveguide rod through a distal side gain step. The intermediate structure comprises N (N>0, and N is an integer) gain holding structures that are connected two by two in a position near an antinode of the longitudinal vibration of the waveguide rod through an intermediate gain step. X (X>0, and X is an integer) frequency adjustment structures exist on the gain holding structure.

Abstract: The present application provides a reusable ultrasonic surgical instrument, comprising a cannula subassembly, a scalpel waveguide, a base and a clamping driving component, wherein the cannula subassembly comprises a pair of clamp forceps at a distal end, an external cannula and an internal cannula, and the external cannula and the internal cannula are both provided by being coaxial with the scalpel bar; two rotating shafts are provided at a proximal end of the clamp forceps, one of the two rotating shafts is rotatably connected with the external cannula, the other rotating shaft is rotatably connected with the internal cannula; the cannula subassembly can be dismounted from the base and the clamping driving component towards a distal end direction of the axis of the scalpel bar or be mounted on the base or the clamping driving component towards a proximal end direction of the axis of the scalpel bar; the external cannula is connected with the base by one or multiple first detachable structures; and the intern

Abstract: A waveguide rod for an ultrasonic scalpel having a relatively desirable amplitude and frequency comprises a proximal gain structure, a distal gain structure, an intermediate structure, and a frequency adjustment structure. The proximal gain structure and the intermediate structure are connected in a position near an antinode of longitudinal vibration of the waveguide rod through a proximal side gain step. The distal gain structure and the intermediate structure are connected in a position near an antinode of the longitudinal vibration of the waveguide rod through a distal side gain step. The intermediate structure comprises N (N>0, and N is an integer) gain holding structures that are connected two by two in a position near an antinode of the longitudinal vibration of the waveguide rod through an intermediate gain step. X (X>0, and X is an integer) frequency adjustment structures exist on the gain holding structure.