Patents by Inventor Ruizhi HAN

Ruizhi HAN has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20240200039
    Abstract: The disclosure discloses a cyclodextrin glycosyltransferase with enhanced solvent tolerance and preparation thereof, belonging to the technical fields of enzyme engineering and genetic engineering. The disclosure constructs four cyclodextrin glycosyltransferase mutants with enhanced organic solvent tolerance. Among them, the mutant with the optimal tolerance to DMSO and methanol is G539I/R146F/D147N, which is 1.6 times and 1.7 times higher than that of WT, respectively; the mutant with the optimal tolerance to ethanol is R146F, which is 1.4 times higher than that of WT; the mutant with the optimal tolerance to acetone is G539I/R146F, which is 1.5 times higher than that of WT. The disclosure helps to expand the application of glycosyltransferases in organic reaction systems, improves the enzymatic efficiency of CGTase on natural hydrophobic substrates, and has great application prospects.
    Type: Application
    Filed: January 25, 2024
    Publication date: June 20, 2024
    Inventors: Ruizhi HAN, Ye NI, Yulin JIANG, Zhaoyue FAN
  • Patent number: 11542479
    Abstract: The invention discloses an alcohol dehydrogenase mutant and use thereof. The alcohol dehydrogenase mutant of the present invention has high thermal stability and enables high catalytic efficiency and high conversion rate (i.e. space time yield) in the asymmetric reduction of prochiral diaryl ketones to produce chiral diaryl alcohols. Therefore, the alcohol dehydrogenase mutant of the present invention has extremely high prospect of application in the production of chiral diaryl alcohols, such as (S)-(4-chlorophenyl)-(pyridin-2-yl)-methanol, (R)-(4-chlorophenyl)-(pyridin-2-yl)-methanol.
    Type: Grant
    Filed: May 13, 2020
    Date of Patent: January 3, 2023
    Assignee: JIANGNAN UNIVERSITY
    Inventors: Ye Ni, Wei Dai, Guochao Xu, Jieyu Zhou, Ruizhi Han, Jinjun Dong
  • Patent number: 11203743
    Abstract: The disclosure discloses a method for producing long-chain glycosylated genistein and belongs to the technical fields of enzyme engineering and fermentation engineering. The disclosure provides a method for producing long-chain glycosylated genistein. By using this method to produce long-chain glycosylated genistein, the content of long-chain glycosylated genistein in a reaction solution and the ratio of the content of long-chain glycosylated genistein in the reaction solution to the content of total glycosylated genistein in the reaction solution can be increased. The content of long-chain glycosylated genistein in the reaction solution can be increased to 10.3 g/L, and the ratio of the content of long-chain glycosylated genistein in the reaction solution to the content of total glycosylated genistein in the reaction solution can be increased to 70%.
    Type: Grant
    Filed: December 16, 2020
    Date of Patent: December 21, 2021
    Assignee: Jiangnan University
    Inventors: Ruizhi Han, Ye Ni, Baocheng Chai
  • Publication number: 20210363500
    Abstract: The invention discloses an alcohol dehydrogenase mutant and use thereof. The alcohol dehydrogenase mutant of the present invention has high thermal stability and enables high catalytic efficiency and high conversion rate (i.e. space time yield) in the asymmetric reduction of prochiral diaryl ketones to produce chiral diaryl alcohols. Therefore, the alcohol dehydrogenase mutant of the present invention has extremely high prospect of application in the production of chiral diaryl alcohols, such as (S)-(4-chlorophenyl)-(pyridin-2-yl)-methanol, (R)-(4-chlorophenyl)-(pyridin-2-yl)-methanol.
    Type: Application
    Filed: May 13, 2020
    Publication date: November 25, 2021
    Inventors: Ye NI, Wei DAI, Guochao XU, Jieyu ZHOU, Ruizhi HAN, Jinjun DONG
  • Publication number: 20210108182
    Abstract: The disclosure discloses a method for producing long-chain glycosylated genistein and belongs to the technical fields of enzyme engineering and fermentation engineering. The disclosure provides a method for producing long-chain glycosylated genistein. By using this method to produce long-chain glycosylated genistein, the content of long-chain glycosylated genistein in a reaction solution and the ratio of the content of long-chain glycosylated genistein in the reaction solution to the content of total glycosylated genistein in the reaction solution can be increased. The content of long-chain glycosylated genistein in the reaction solution can be increased to 10.3 g/L, and the ratio of the content of long-chain glycosylated genistein in the reaction solution to the content of total glycosylated genistein in the reaction solution can be increased to 70%.
    Type: Application
    Filed: December 16, 2020
    Publication date: April 15, 2021
    Inventors: Ruizhi HAN, Ye NI, Baocheng CHAI