Patents by Inventor Runxia Li

Runxia Li has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Method for characterizing biological part based on dual-fluorescent reporter gene system and biological part library constructed thereon
    Patent number: 11248258
    Abstract: A method for identifying and characterizing biological parts based on omics datasets and a dual-fluorescent reporter gene system, and a biological part library constructed thereon are provided, relating to a technical filed of biology. The method includes steps of: identifying the biological parts using the omics datasets; constructing a single-fluorescent reporter gene system using a shuttle vector pEZ15Asp as a skeleton for screening and determining fluorescent reporter genes; obtaining a dual-fluorescent reporter gene system skeleton; constructing recombinant plasmids, and finally transforming into competent cells for quantitative analysis of fluorescence intensities. The present invention is convenient and quick, and can screen and identify different biological parts such as RBS, UTRs, promoters, and terminators of different intensities in batch quantitatively in a relatively short time. Moreover, the present invention can quickly expand the biological part library of Z.
    Type: Grant
    Filed: May 9, 2019
    Date of Patent: February 15, 2022
    Assignee: Hubei University
    Inventors: Shihui Yang, Yongfu Yang, Wei Shen, Runxia Li, Ju Huang, Yu Wang, Li Yi, Lixin Ma
  • Method for identifying biological parts based on dual-fluorescent reporter gene system and biological part library constructed thereon
    Publication number: 20200385795
    Abstract: A method for identifying and characterizing biological parts based on omics datasets and a dual-fluorescent reporter gene system, and a biological part library constructed thereon are provided, relating to a technical filed of biology. The method includes steps of: identifying the biological parts using the omics datasets; constructing a single-fluorescent reporter gene system using a shuttle vector pEZ15Asp as a skeleton for screening and determining fluorescent reporter genes; obtaining a dual-fluorescent reporter gene system skeleton; constructing recombinant plasmids, and finally transforming into competent cells for quantitative analysis of fluorescence intensities. The present invention is convenient and quick, and can screen and identify different biological parts such as RBS, UTRs, promoters, and terminators of different intensities in batch quantitatively in a relatively short time. Moreover, the present invention can quickly expand the biological part library of Z.
    Type: Application
    Filed: May 9, 2019
    Publication date: December 10, 2020
    Inventors: Shihui Yang, Yongfu Yang, Wei Shen, Runxia Li, Ju Huang, Yu Wang, Li Yi, Lixin Ma