Abstract: Embodiments are directed to engineered bacteriophages that produce polypeptides that interfere with or quench quorum sensing. In certain aspects the quorum quenching enzyme AiiA has a broad specificity for degrading substrates, small signal molecule acyl homoserine factories (AHL) that initiates the quorum sensing pathway with global impact for diverse bacteria in biofilm. Certain embodiments are directed to the engineered T7aiiA phage that effectively degraded AHL from diverse bacteria and inhibited formation of the mixed species biofilms containing Pseudomonas aeruginosa and E. coli. Engineered phages can be used as antifouling and antibacterial agents in industrial and clinical settings by lysing the host bacteria and stably expressing quorum quenching enzyme having a broad substrate specificity and impacting diverse bacteria in the community.