Abstract: Provided herein are systems and methods for tissue characterization that may be utilized for intraoperative cancer margin assessment. A tissue sample is resected from a patient and then placed in a system where it is imaged for autofluorescence characteristics to help identify regions of interest such as cancerous tissue margins. Based on the imaging results and tissue characterization, the resection of tissue in the subject can be adjusted.

Abstract: A method for imaging an object with resolution that exceeds the number of spatial modes per polarization in a multimode fiber is disclosed. In some embodiments, the object is interrogated with a plurality of non-spot-sized intensity patterns and the optical power reflected by the object is detected for each intensity pattern. The plurality of optical power values is then used in a non-local reconstruction based on an optimization approach to reconstruct an image of the object, where the image has resolution up to four times greater than provided by prior-art multimode fiber-based imaging methods.