Abstract: Disclosed is a method for determining the age of ginseng roots using chromatography-mass spectroscopy. It comprises: extracting a metabolome from a ginseng sample; subjecting the metabolome to liquid chromatography-mass spectroscopy (LC/MS) or gas chromatography-mass spectroscopy (GC/MS) to afford an analysis result; converting the LC/MS or GC/MS analysis result to statistically accessible data; and performing a statistical analysis of the data to determine the age of ginseng sample. Based on the metabolite fingerprinting of metabolomics, the method can determine the exact age of ginseng roots from a very small amount of roots within a short time in a non-destructive manner with minimal damage to the roots.