Patents by Inventor Russell Gene Higuchi

Russell Gene Higuchi has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • System and method for detection of HLA Variants
    Publication number: 20100261189
    Abstract: A method for detecting one or more HLA sequence types is described that comprises the steps of: amplifying a plurality of first amplicons from a double stranded nucleic acid sample, wherein the first amplicons are amplified with a plurality of pairs of nucleic acid primers that define exons 2 and 3 of both strands of HLA loci from the group consisting of HLA-A, HLA-B, and HLA-C; amplifying the first amplicons to produce a plurality of populations of second amplicons, wherein each population of second amplicons is clonally amplified from one of the first amplicons; sequencing the plurality of populations of second amplicons to generate a nucleic acid sequence composition for each of the plurality of second amplicons; and detecting variation in the sequence composition from one or more of the second amplicons for one or more of the HLA loci.
    Type: Application
    Filed: April 12, 2010
    Publication date: October 14, 2010
    Inventors: Gordon Bentley, Henry A. Erlich, Russell Gene Higuchi, Cherie Holcomb
  • Associations of polymorphisms in the FRZB gene in obesity and osteoporosis
    Patent number: 7790371
    Abstract: The present invention relates to polymorphisms of the FRZB gene and the analysis of nucleic acids thereof. The invention further relates to methods of predicting risks of certain conditions in individual and kits for the determination of such risks.
    Type: Grant
    Filed: April 20, 2004
    Date of Patent: September 7, 2010
    Assignee: Roche Molecular Systems, Inc.
    Inventors: Russell Gene Higuchi, Gary Allen Peltz, Bonnie Fijal, Sunhee Kwon Ro, Jia Li
  • High temperature reverse transcription using mutant DNA polymerases
    Patent number: 7179590
    Abstract: The present invention relates to improved reverse transcription methods using a modified thermostable DNA polymerases, particularly in a magnesium ion buffer. These methods are particularly useful in combined reverse-transcription/amplification reactions.
    Type: Grant
    Filed: March 30, 2001
    Date of Patent: February 20, 2007
    Assignee: Roche Molecular Systems, Inc
    Inventors: Edward Soh Smith, Carita Maria Elfstrom, David Harrow Gelfand, Russell Gene Higuchi, Thomas William Myers, Nancy Jeneane Schönbrunner, Alice Ming Wang
  • Instrument for monitoring nucleic acid amplification
    Patent number: 6814934
    Abstract: This invention relates to improved methods for nucleic acid detection using methods such as the polymerase chain reaction (PCR). More specifically, the invention provides methods for simultaneous amplification and detection to enhance the speed and accuracy of prior methods. The methods involve the introduction of detectable DNA binding agents into the amplification reaction, which agents produce a detectable signal that is enhanced upon binding double-stranded DNA. In a preferred embodiment, the binding agent is a fluorescent dye. The methods also provide means for monitoring the increase in product DNA during an amplification reaction.
    Type: Grant
    Filed: November 12, 1997
    Date of Patent: November 9, 2004
    Inventor: Russell Gene Higuchi
  • High temperature reverse transcription using mutant DNA polymerases
    Publication number: 20020012970
    Abstract: The present invention relates to improved reverse transcription methods using a modified thermostable DNA polymerases, particularly in a magnesium ion buffer. These methods are particularly useful in combined reverse-transcription/amplification reactions.
    Type: Application
    Filed: March 30, 2001
    Publication date: January 31, 2002
    Inventors: Edward Soh Smith, Carita Maria Elfstrom, David Harrow Gelfand, Russell Gene Higuchi, Thomas William Myers, Nancy Jeneane Schonbrunner, Alice Ming Wang