Abstract: Described herein are block copolymers, and methods of making and utilizing such copolymers. The described block copolymers are disruptive of a cellular membrane, including an extracellular membrane, an intracellular membrane, a vesicle, an organelle, an endosome, a liposome, or a red blood cell. Preferably, in certain instances, the block copolymer disrupts the membrane and enters the intracellular environment. In specific examples, the block copolymer is endosomolytic and capable of delivering an oligonucleotide (e.g., an mRNA) to a cell. Compositions comprising a block copolymer and an oligonucleotide (e.g., an mRNA) are also disclosed.

Abstract: An automatically controlled Chub packaging machine wherein the product delivery pump, tube or film feed, and clamping mechanism of the vertically reciprocating clipping head, are automatically controlled by means of servo actuators operatively connected to a microprocessor. The microprocessor of the automatic control system also coordinates the functioning of the film heat sealer and the voider. An operator interface membrane panel with video display allows the operator to set, observe and adjust the various functions of the machine. The microprocessor system links all elements together and places various rates and set points in memory for immediate recall.