Abstract: Production of DNA vectors with the inserted gene of a target protein and the production of recombinant protein in eukaryotic cell cultures is disclosed. A composition for the intensive production of target protein in eukaryotic cells comprises a DNA vector with the inserted gene of a target protein and an agonist of cell receptors belonging to the pattern recognition receptor (PRR) family selected from the following agonists: TLR2, or TLR4, or TLR5, or TLR7, or TLR8, or TLR9, or NOD1 receptor, or NOD2 receptor, used in an optimal ratio. TLR2 is a lipoteichoic acid. TLR2 is a lipopeptide. TLR4 can be either bacterial lipopolysaccharide or acidic peptidoglycan (APG) having a molecular weight of 1200-40000 kDa. TLR5 is flagellin TLR7 is either imiquimod or CL097, an imidazoquinoline derivative. TLR8 is either imiquimod or CL097, an imidazoquinoline derivative. TLR9 is either oligonucleotide CpG ODN 1826 or oligonucleotide CpG ODN 2006.

Abstract: A pharmaceutical composition and method for therapy of neurodegenerative diseases including amyotrophic lateral sclerosis, the composition comprising an adenoviral vector, expressing a human angiogenesis factor gene; an effective amount of the adenoviral vector in the form of a non-replicating nanoparticle based on a genome of human adenovirus type 5 with an insertion of a human angiogenin gene which produces the human angiogenesis in a human body; a non-replicating nanoparticle based on the genome of human adenovirus type 5 with an insertion of a human vascular endothelium growth factor gene; and a formulating buffer; wherein the human angiogenin factor gene and the human vascular endothelium growth factor gene are cloned in two expression cassettes within one non-replicating nanoparticle based on the genome of human adenovirus type 5.

Abstract: The pharmaceutical composition producing the antioxidant, antimicrobal, antitoxic protein—human lactoferrin in which the therapeutic effect is achieved as a result of the antioxidant, antimicrobal, antitoxic protein—human lactoferrin on the human body different in what it contains human adenovirus 5 genome based non-replicating nanoparticles with the human lactoferrin gene insert expressing human lactoferrin in a therapeutically effective amount in the body and containing the formulating buffer. The non-replicating nanoparticles content makes no less than 2.33×1011 virus particle (v.p.) per ml of the formulating buffer.

Abstract: A pharmaceutical composition for treatment of acute toxic conditions relates to the field of medicine, particularly to nanotechnology and toxicology, and can be used for prophylaxis and therapy of various etiologies of toxic states, including the acute ones. The claimed pharmaceutical composition for treatment of acute toxic conditions contains protein—the human lactoferrin—and further comprises of non-replicating nanoparticles with inset of human lactoferrin gene and formulating buffer. The dose of the claimed pharmaceutical composition is 3 ml. The dose of the claimed pharmaceutical composition comprises: human lactoferrin—from 50 to 100 mg; non-replicating nanoparticles—7×1011 virus particle (v.p.); formulating buffer—rest, ml. At the same time, the donor human milk lactoferrin or any human lactoferrin is used as the human lactoferrin.

Abstract: Recombinant trivalent vaccine against human influenza contains recombinant adenoviral vector with a nucleotide sequence which encodes at least one antigen of influenza virus strain, while non-replicating nanoparticles are used as adenoviral vectors based on genome of human adenovirus of serotype 5, capable of expressing the haemagglutinin of influenza virus which induce human immune response to influenza virus, wherein the vaccine contains composition of three kinds of non-replicating nanoparticles, each of which carries different influenza hemagglutinin genes, further comprises immunostimulant and formulating buffer. In this solution the influenza virus hemagglutinin genes are: H1, H3, HB and any other recommended by the World Health Organization at the time of production. Formulating buffer is added to the composition to achieve a total volume of 0.5 ml and a vaccine dose of 0.5 ml.