Abstract: The invention provides an automated VNTR genotyping method using multiple variable-number tandem repeats (VNTRs) loci based on mycobacterial interspersed repetitive units (MIRU) undergoing multiplex PGR and high throughput MEGABACE® capillary electrophoresis system. The method uses fluorescent dyes of 6-carboxytetramethylrhodamine(TAMRA), 6-carboxy fluorescein (FAM) and 6-carboxy-2?, 4, 4?, 5?, 7, 7?-hexachlorofluorescein (HEX) labeling PGR primers. The method results in an efficient VNTR genotyping with low cost, less labor-requirement and less reaction time. The method is applicable in organism analyses by VNTR genotyping, such as microorganisms, parasites, animals or plants.

Abstract: The invention provides an automated VNTR genotyping method using multiple variable-number tandem repeats (VNTRs) loci based on mycobacterial interspersed repetitive units (MIRU) undergoing multiplex PCR and high throughput MegaBACE® capillary electrophoresis system. The method uses fluorescent dyes of 6-carboxytetramethylrhodamine(TAMRA), 6-carboxy fluorescein (FAM) and 6-carboxy-2?,4,4?,5?,7,7?-hexachlorofluorescein (HEX) labeling PCR primers. The method results in an efficient VNTR genotyping with low cost, less labor-requirement and less reaction time. The method is applicable in organism analyses by VNTR genotyping, such as microorganisms, parasites, animals or plants.

Abstract: This invention provides a process for large-scale purification of living Japanese encephalitis virus (JEV) suitable for use in vaccine preparation from a JEV source, including a JEV-infected cell culture and a JEV-infected mouse brain. The process includes the following steps: (a) obtaining a sample from a JEV-infected mouse brain or a JEV-infected cell culture, (b) subjecting the sample to a preliminary separation to remove cell and cell debris from the sample of step (a), (c) concentrating the sample from step (b) by ultra-filtration to remove substances having molecular weight below 100 kDa, and (d) subjecting the sample concentrate to gel filtration to obtain a substantially pure fraction of JEV.