Abstract: Provided herein is a method of characterising a target polynucleotide as it moves with respect to a nanopore using a motor protein. Also provided are polynucleotide adapters and kits comprising such adapters. The methods, kits and adapters find use in characterising polynucleotides, for example in sequencing.

Abstract: A rolling luggage bag includes a cover fixedly coupled to a base, the base including a first side opposite a second side and a bottom face extending there between, wherein the cover comprises a plurality of panels, arranged in a direction away from the base, wherein the plurality of panels defines an interior chamber, the interior chamber comprising an internal cover fixedly attached to the panels of the fifth panel zone, a first wheel and a second wheel coupled to the base, the first and second wheels configured to rotate about an axis of rotation and separated by a first distance along the axis of rotation, at least a portion of each of the first and second wheels projecting from the first side and from the bottom face, and a third wheel and a fourth wheel coupled to the bottom face, the third and fourth wheels configured to independently swivel about a respective swivel axis and separated by a second distance extending between the swivel axes.

Abstract: Described herein is a golf bag with a sub-assembly and a stand assembly that together form a carry bag. The sub-assembly can comprise a divider top, a base, a divider sleeve, one or more stays that secure the base to the divider top, and a flat. The stand assembly can comprise a pair of legs, a spring, a pair of brackets for attaching the spring to the legs, and a pair of leg end cap protectors. When strapped to a golf cart, a golf bag can rub against portions of the cart, wearing paint off the legs. The instant golf bag comprises leg end cap protectors that can prevent wear on an upper portion of the legs. Furthermore, the stand assembly can be shipped disconnected from the sub-assembly to allow the sub-assembly to collapse and reduce shipping box size.

Abstract: A golf bag includes a collapsible sub-assembly including a divider top and a base, a plurality of first snap-fit connectors provided around a portion of an outer perimeter of the divider top, and a flat having a divider top end opposite a base end, an interior side, and an exterior side. The flat includes a plurality of second snap-fit connectors provided along a portion of the divider top end. The flat attaches to the sub-assembly by mating snap-fit engagement of the plurality of first snap-fit connectors around the divider top with the plurality of second snap-fit connectors along the divider top end.

Abstract: Described herein is a golf bag with a sub-assembly and a stand assembly that together form a carry bag. The sub-assembly can comprise a divider top, a base, a divider sleeve, one or more stays that secure the base to the divider top, and a flat. The stand assembly can comprise a pair of legs, a spring, a pair of brackets for attaching the spring to the legs, and a pair of leg end cap protectors. When strapped to a golf cart, a golf bag can rub against portions of the cart, wearing paint off the legs. The instant golf bag comprises leg end cap protectors that can prevent wear on an upper portion of the legs. Furthermore, the stand assembly can be shipped disconnected from the sub-assembly to allow the sub-assembly to collapse and reduce shipping box size.

Abstract: A golf bag includes a collapsible sub-assembly including a divider top and a base, a plurality of first snap-fit connectors provided around a portion of an outer perimeter of the divider top, and a flat having a divider top end opposite a base end, an interior side, and an exterior side. The flat includes a plurality of second snap-fit connectors provided along a portion of the divider top end. The flat attaches to the sub-assembly by mating snap-fit engagement of the plurality of first snap-fit connectors around the divider top with the plurality of second snap-fit connectors along the divider top end.

Abstract: A fire rated glass flooring system having blast and/or seismic loading resistance, comprising: (a) a plurality of glass flooring units (100a, 100b), each unit comprising a first layer (116) of glass and a second layer (118) of glass, the two layers being positioned one above the other and separated by one or more load transferring means (120a, 120b), wherein the first layer of glass is a structural glass and the second layer of glass is a fire rated glass, and having an upper surface and an edge comprising a load-transferring means of the one or more load transferring means; (b) one or more beams (112), arranged, in use, to support the units, wherein, at a boundary between two adjacent flooring units, the two flooring units are arranged, in use, to be secured to a beam of the one or more beams; and (c) one or more expansion joints (110), each arranged, in use, at the boundary between two adjacent flooring units.

Abstract: A rolling luggage bag includes a cover fixedly coupled to a base, the base including a first side opposite a second side and a bottom face extending there between, wherein the cover comprises a plurality of panels, arranged in a direction away from the base, wherein the plurality of panels defines an interior chamber, the interior chamber comprising an internal cover fixedly attached to the panels of the fifth panel zone, a first wheel and a second wheel coupled to the base, the first and second wheels configured to rotate about an axis of rotation and separated by a first distance along the axis of rotation, at least a portion of each of the first and second wheels projecting from the first side and from the bottom face, and a third wheel and a fourth wheel coupled to the bottom face, the third and fourth wheels configured to independently swivel about a respective swivel axis and separated by a second distance extending between the swivel axes.

Abstract: A rolling luggage bag includes a cover fixedly coupled to a base, the base including a first side opposite a second side and a bottom face extending there between, wherein the cover comprises a plurality of panels, arranged in a direction away from the base, wherein the plurality of panels defines an interior chamber, the interior chamber comprising an internal cover fixedly attached to the panels of the fifth panel zone, a first wheel and a second wheel coupled to the base, the first and second wheels configured to rotate about an axis of rotation and separated by a first distance along the axis of rotation, at least a portion of each of the first and second wheels projecting from the first side and from the bottom face, and a third wheel and a fourth wheel coupled to the bottom face, the third and fourth wheels configured to independently swivel about a respective swivel axis and separated by a second distance extending between the swivel axes.

Abstract: A sub-assembly for a golf bag includes a divider top having an outer ring and a plurality of apertures, and a leg mounting bracket having a mounting channel and a plurality of retention members extending into the mounting channel. A portion of the outer ring is received in the mounting channel and each of the plurality of apertures receives one of the plurality of retention members to form a snap-fit connection.

Abstract: A golf bag includes a collapsible sub-assembly including a divider top and a base, a plurality of first snap-fit connectors provided around a portion of an outer perimeter of the divider top, and a flat having a divider top end opposite a base end, an interior side, and an exterior side. The flat includes a plurality of second snap-fit connectors provided along a portion of the divider top end. The flat attaches to the sub-assembly by mating snap-fit engagement of the plurality of first snap-fit connectors around the divider top with the plurality of second snap-fit connectors along the divider top end.

Abstract: A rolling luggage bag includes a cover coupled to a base, the base including a first side opposite a second side and a bottom face extending there between, a first wheel and a second wheel coupled to the base, the first and second wheels configured to rotate about an axis of rotation and separated by a first distance along the axis of rotation, at least a portion of each of the first and second wheels projecting from the first side and from the bottom face, and a third wheel and a fourth wheel coupled to the bottom face, the third and fourth wheels configured to independently swivel about a respective swivel axis and separated by a second distance extending between the swivel axes. When the rolling luggage is in an upright position, the first, second, third, and fourth wheels all contact a surface the luggage bag stands on.

Abstract: A golf bag includes a divider top, a base, and a plurality of stays comprising a first stay, a second stay, and a main stay having a first end opposite a second end. The golf bag further comprises a top hinge coupled to the first end of the main stay. A base hinge can be coupled to the second end of the main stay, wherein the main stay can be hingedly connected to the divider top by the top hinge, and the stay can be hingedly connected to the base by the base hinge. Other embodiment of the golf bag are also described.

Abstract: A golf bag includes a collapsible sub-assembly including a divider top and a base, a plurality of first snap-fit connectors provided around a portion of an outer perimeter of the divider top, and a flat having a divider top end opposite a base end, an interior side, and an exterior side. The flat includes a plurality of second snap-fit connectors provided along a portion of the divider top end. The flat attaches to the sub-assembly by mating snap-fit engagement of the plurality of first snap-fit connectors around the divider top with the plurality of second snap-fit connectors along the divider top end.

Abstract: A fire rated glass flooring system having blast and/or seismic loading resistance, comprising: (a) a plurality of glass flooring units (100a, 100b), each unit comprising a first layer (116) of glass and a second layer (118) of glass, the two layers being positioned one above the other and separated by one or more load transferring means (120a, 120b), wherein the first layer of glass is a structural glass and the second layer of glass is a fire rated glass, and having an upper surface and an edge comprising a load-transferring means of the one or more load transferring means; (b) one or more beams (112), arranged, in use, to support the units, wherein, at a boundary between two adjacent flooring units, the two flooring units are arranged, in use, to be secured to a beam of the one or more beams; and (c) one or more expansion joints (110), each arranged, in use, at the boundary between two adjacent flooring units.

Abstract: Embodiments of bag having an adjustable carrying strap system and methods to manufacture such a bag are generally described herein. In some embodiments, the adjustable carrying strap system includes a plurality of carrying straps configured to be coupled to a bag at one end and a connector plate at the opposite end thereof to provide a self-centering capability to the bag, such as a golf bag. The connector plate comprises an arc region and an apex region opposite the arc region and defines a first arc slot and a second arc slot in the arc region and a first straight slot and a second straight slot within the apex region. In some embodiments, the connector plate defines a center slot defined between the first and second arc slots and the first and second straight slots.

Abstract: Embodiments of bag having an adjustable carrying strap system and methods to manufacture such a bag are generally described herein. In some embodiments, the adjustable carrying strap system includes a plurality of carrying straps configured to be coupled to a bag at one end and a connector plate at the opposite end thereof to provide a self-centering capability to the bag, such as a golf bag. The connector plate comprises an arc region and an apex region opposite the arc region and defines a first arc slot and a second arc slot in the arc region and a first straight slot and a second straight slot within the apex region. In some embodiments, the connector plate defines a center slot defined between the first and second arc slots and the first and second straight slots.

Abstract: Described herein is a golf bag with a sub-assembly and a stand assembly that together form a carry bag. The sub-assembly can comprise a divider top, a base, a divider sleeve, one or more stays that secure the base to the divider top, and a flat. The stand assembly can comprise a pair of legs, a spring, a pair of brackets for attaching the spring to the legs, and a pair of leg end cap protectors. When strapped to a golf cart, a golf bag can rub against portions of the cart, wearing paint off the legs. The instant golf bag comprises leg end cap protectors that can prevent wear on an upper portion of the legs. Furthermore, the stand assembly can be shipped disconnected from the sub-assembly to allow the sub-assembly to collapse and reduce shipping box size.

Abstract: A golf bag includes a collapsible sub-assembly including a divider top and a base, a plurality of first snap-fit connectors provided around a portion of an outer perimeter of the divider top, and a flat having a divider top end opposite a base end, an interior side, and an exterior side. The flat includes a plurality of second snap-fit connectors provided along a portion of the divider top end. The flat attaches to the sub-assembly by mating snap-fit engagement of the plurality of first snap-fit connectors around the divider top with the plurality of second snap-fit connectors along the divider top end.

Abstract: A tensioning device for a sheet of material that includes a first ring with protrusions, a second ring, and a shaft. The first ring and the second ring are positioned such that the sheet of material can be clamped between the first ring and the second ring. The shaft has an outer surface, the outer surface includes a spiral race such that the protrusions of the first ring follow the spiral race, allowing the first ring and the second ring to displace linearly causing the sheet of material to be stretched. The spiral race includes a plurality of notches that correspond to the protrusions.