Abstract: The present disclosure relates to polymer coatings covalently attached to the surface of a substrate and the preparation of the polymer coatings, such as poly(N-(5-azidoacetamidylpentyl)acrylamide-co-acrylamide) (PAZAM), in the formation and manipulation of substrates, such as molecular arrays and flow cells. The present disclosure also relates to methods of preparing a substrate surface by using beads coated with a covalently attached polymer, such as PAZAM, and the method of determining a nucleotide sequence of a polynucleotide attached to a substrate surface described herein.

Abstract: A method of coating a glass substrate with a hot end coating is provided. The method comprises a step of applying an aqueous dispersion including particles of a metal hydroxide composition onto a surface of the glass substrate to form a hot end coating on the glass substrate. The glass surface has a temperature of from about 450° C. to about 1000° C. when the aqueous dispersion is applied thereto. After application, the hot end coating includes oxide(s) of the metal or combinations thereof. A coated glass article is also provided. The coating on the glass article includes at least one of Group 3 A oxides, Group 4A oxides, or combinations thereof; preferably TiO2, TiO, Ti2O3, TizOa (0<z?2 and 0<a?3), ZrO2, ZrbOc (0<b?1 and 0<c?2), Al2O3, Al2O, AlO, AlxOy (0<x?2 and 0<y?3) or combinations thereof; more preferably Al2O3, Al2O, AlO, AlxOy (0<x?2 and 0<y?3) or combinations thereof.

Abstract: A method of coating a glass substrate with a hot end coating is provided. The method includes a step of applying a composition comprising at least one organic salt of a metal onto a surface of the glass substrate to form a hot end coating on the glass substrate. The glass surface has a temperature of from about 450° C. to about 800° C. when the composition comprising at least one organic salt of a metal is applied thereto. After application the hot end coating includes oxide(s) of the metal, or combinations thereof. A coated glass article is also provided. The coating on the glass article is a hot end coating including at least one of Group 3A oxides, Group 4A oxides, or combinations thereof.

Abstract: A footwear device comprising protrusions which extend into a surface provide increased leverage when a force is required to be applied in a plane about perpendicular to the protrusions and about parallel to the surface. In particular, footwear devices can increase leverage in a sport requiring increased leverage to essentially maintain contact with the footwear and the ground, such as but not limited to golf.

Abstract: Embodiments of the present disclosure include methods and compositions for functionalizing molecules, such as oligonucleotides, with functional groups, including polyhistidine tags useful in affinity methods. Some embodiments include methods for modifying and purifying complex mixtures of molecules by exchange of functional tags.

Abstract: The present disclosure relates to polymer coatings covalently attached to the surface of a substrate and the preparation of the polymer coatings, such as poly(N-(5-azidoacetamidylpentyl)acrylamide-co-acrylamide) (PAZAM), in the formation and manipulation of substrates, such as molecular arrays and flow cells. The present disclosure also relates to methods of preparing a substrate surface by using beads coated with a covalently attached polymer, such as PAZAM, and the method of determining a nucleotide sequence of a polynucleotide attached to a substrate surface described herein.

Abstract: The present disclosure relates to polymer coatings covalently attached to the surface of a substrate and the preparation of the polymer coatings, such as poly(N-(5-azidoacetamidylpentyl)acrylamide-co-acrylamide) (PAZAM), in the formation and manipulation of substrates, such as molecular arrays and flow cells. The present disclosure also relates to methods of preparing a substrate surface by using beads coated with a covalently attached polymer, such as PAZAM, and the method of determining a nucleotide sequence of a polynucleotide attached to a substrate surface described herein.

Abstract: Embodiments of the present disclosure include methods and compositions for functionalizing molecules, such as oligonucleotides, with functional groups, including polyhistidine tags useful in affinity methods. Some embodiments include methods for modifying and purifying complex mixtures of molecules by exchange of functional tags.

Abstract: The present disclosure relates to polymer coatings covalently attached to the surface of a substrate and the preparation of the polymer coatings, such as poly(N-(5-azidoacetamidylpentyl)acrylamide-co-acrylamide) (PAZAM), in the formation and manipulation of substrates, such as molecular arrays and flow cells. The present disclosure also relates to methods of preparing a substrate surface by using beads coated with a covalently attached polymer, such as PAZAM, and the method of determining a nucleotide sequence of a polynucleotide attached to a substrate surface described herein.

Abstract: Embodiments of the present disclosure include methods and compositions for functionalizing molecules, such as oligonucleotides, with functional groups, including polyhistidine tags useful in affinity methods. Some embodiments include methods for modifying and purifying complex mixtures of molecules by exchange of functional tags.

Abstract: The present disclosure relates to polymer coatings covalently attached to the surface of a substrate and the preparation of the polymer coatings, such as poly(N-(5-azidoacetamidylpentyl)acrylamide-co-acrylamide) (PAZAM), in the formation and manipulation of substrates, such as molecular arrays and flow cells. The present disclosure also relates to methods of preparing a substrate surface by using beads coated with a covalently attached polymer, such as PAZAM, and the method of determining a nucleotide sequence of a polynucleotide attached to a substrate surface described herein.

Abstract: Embodiments of the present disclosure include methods and compositions for functionalizing molecules, such as oligonucleotides, with functional groups, including polyhistidine tags useful in affinity methods. Some embodiments include methods for modifying and purifying complex mixtures of molecules by exchange of functional tags.

Abstract: The present disclosure relates to polymer coatings covalently attached to the surface of a substrate and the preparation of the polymer coatings, such as poly(N-(5-azidoacetamidylpentyl)acrylamide-co-acrylamide) (PAZAM), in the formation and manipulation of substrates, such as molecular arrays and flow cells. The present disclosure also relates to methods of preparing a substrate surface by using beads coated with a covalently attached polymer, such as PAZAM, and the method of determining a nucleotide sequence of a polynucleotide attached to a substrate surface described herein.

Abstract: Embodiments of the present disclosure include methods and compositions for functionalizing molecules, such as oligonucleotides, with functional groups, including polyhistidine tags useful in affinity methods. Some embodiments include methods for modifying and purifying complex mixtures of molecules by exchange of functional tags.

Abstract: The present disclosure relates to polymer coatings covalently attached to the surface of a substrate and the preparation of the polymer coatings, such as poly(N-(5-azidoacetamidylpentyl)acrylamide-co-acrylamide) (PAZAM), in the formation and manipulation of substrates, such as molecular arrays and flow cells. The present disclosure also relates to methods of preparing a substrate surface by using beads coated with a covalently attached polymer, such as PAZAM, and the method of determining a nucleotide sequence of a polynucleotide attached to a substrate surface described herein.

Abstract: An exemplary embodiment includes a system to determine which wells provide input volumes to gas processing plants using a mixed-integer programming (MIP) model. The system uses a balanced approach to model all plants and wells within a basin simultaneously. The model utilizes multiple publically available data sources including well production data, plant inlet data, and GIS data. The full implementation provides at minimum a tenfold time improvement over the previously used manual techniques while increasing overall model quality.

Abstract: An apparatus for coating glass articles includes a housing structure defining a chamber for receiving air or other fluid from a source of pressurized air that is in fluid communication with the chamber. The housing structure has an open end and a surface defined along at least a portion of the open end. A hot-swappable insert is removably positioned on the surface. The hot-swappable insert has a series of apertures for distributing the air or other fluid from the chamber and onto a surface of the glass articles that are positioned adjacent the insert. The insert can be replaced with another insert to adjust the flow path of the air or other fluid onto the glass articles.

Abstract: Embodiments of the present disclosure include methods and compositions for functionalizing molecules, such as oligonucleotides, with functional groups, including polyhistidine tags useful in affinity methods. Some embodiments include methods for modifying and purifying complex mixtures of molecules by exchange of functional tags.

Abstract: The present disclosure relates to polymer coatings covalently attached to the surface of a substrate and the preparation of the polymer coatings, such as poly(N-(5-azidoacetamidylpentyl)acrylamide-co-acrylamide) (PAZAM), in the formation and manipulation of substrates, such as molecular arrays and flow cells. The present disclosure also relates to methods of preparing a substrate surface by using beads coated with a covalently attached polymer, such as PAZAM, and the method of determining a nucleotide sequence of a polynucleotide attached to a substrate surface described herein.

Abstract: Embodiments of the present disclosure include methods and compositions for functionalizing molecules, such as oligonucleotides, with functional groups, including polyhistidine tags useful in affinity methods. Some embodiments include methods for modifying and purifying complex mixtures of molecules by exchange of functional tags.