Patents by Inventor Ryan Daniel Bomgarden

Ryan Daniel Bomgarden has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Absolute Quantitation of Proteins and Protein Modifications by Mass Spectrometry with Multiplexed Internal Standards
    Publication number: 20210311072
    Abstract: A method for absolute protein or peptide quantitation by mass spectroscopy. A sample containing a protein or peptide of interest is prepared for mass spectroscopy analysis. The sample is subjected to mass spectroscopy analysis at low resolution whereby a single additive mass spectroscopy peak is obtained, then is subjected to high resolution mass spectroscopy analysis whereby a plurality of mass spectroscopy peaks are obtained. The intensity of each of the plurality of mass spectroscopy peaks is quantitated either by comparison to an internal standard set, or by using a standard curve generated for each isotopologue set. Quantitation using a standard curve enhances quantitation across a dynamic range of analyte.
    Type: Application
    Filed: April 6, 2021
    Publication date: October 7, 2021
    Applicant: Pierce Biotechnology, Inc.
    Inventors: Greg Hermanson, John Charles Rogers, Joel Louette, Ryan Daniel Bomgarden, Bhavinkumar Patel
  • Methods and reagents for biomolecule labeling, enrichment and gentle elution
    Patent number: 9835629
    Abstract: Methods and sets of non-biological reagents (elution reagents, tag isomers, tag reactive groups, crosslinkers) for single or multiplexed capture and gentle elution of biomolecules. Examples are provided using amine- and cysteine-reactive reagents for enrichment of proteins, peptides, and rare peptide modifications.
    Type: Grant
    Filed: March 11, 2013
    Date of Patent: December 5, 2017
    Assignee: Pierce Biotechnology, Inc.
    Inventors: John Charles Rogers, Ryan Daniel Bomgarden, Christopher Laurence Etienne, Eric Leigh Hommema
  • Absolute Quantitation of Proteins and Protein Modifications by Mass Spectrometry with Multiplexed Internal Standards
    Publication number: 20160154006
    Abstract: A method for absolute protein or peptide quantitation by mass spectroscopy. A sample containing a protein or peptide of interest is prepared for mass spectroscopy analysis. The sample is subjected to mass spectroscopy analysis at low resolution whereby a single additive mass spectroscopy peak is obtained, then is subjected to high resolution mass spectroscopy analysis whereby a plurality of mass spectroscopy peaks are obtained. The intensity of each of the plurality of mass spectroscopy peaks is quantitated either by comparison to an internal standard set, or by using a standard curve generated for each isotopologue set. Quantitation using a standard curve enhances quantitation across a dynamic range of analyte.
    Type: Application
    Filed: December 28, 2015
    Publication date: June 2, 2016
    Inventors: Greg Hermanson, John Charles Rogers, Joel Louette, Ryan Daniel Bomgarden, Bhavinkumar Patel
  • Absolute quantitation of proteins and protein modifications by mass spectrometry with multiplexed internal standards
    Patent number: 9252003
    Abstract: A method for absolute protein or peptide quantitation by mass spectroscopy. A sample containing a protein or peptide of interest is prepared for mass spectroscopy analysis. The sample is subjected to mass spectroscopy analysis at low resolution whereby a single additive mass spectroscopy peak is obtained, then is subjected to high resolution mass spectroscopy analysis whereby a plurality of mass spectroscopy peaks are obtained. The intensity of each of the plurality of mass spectroscopy peaks is quantitated either by comparison to an internal standard set, or by using a standard curve generated for each isotopologue set. Quantitation using a standard curve enhances quantitation across a dynamic range of analyte.
    Type: Grant
    Filed: June 6, 2014
    Date of Patent: February 2, 2016
    Assignee: Pierce Biotechnology, Inc.
    Inventors: Greg Hermanson, John Charles Rogers, Joel Louette, Ryan Daniel Bomgarden, Bhavinkumar Patel
  • ABSOLUTE QUANTITATION OF PROTEINS AND PROTEIN MODIFICATIONS BY MASS SPECTROMETRY WITH MULTIPLEXED INTERNAL STANDARDS
    Publication number: 20140364337
    Abstract: A method for absolute protein or peptide quantitation by mass spectroscopy. A sample containing a protein or peptide of interest is prepared for mass spectroscopy analysis. The sample is subjected to mass spectroscopy analysis at low resolution whereby a single additive mass spectroscopy peak is obtained, then is subjected to high resolution mass spectroscopy analysis whereby a plurality of mass spectroscopy peaks are obtained. The intensity of each of the plurality of mass spectroscopy peaks is quantitated either by comparison to an internal standard set, or by using a standard curve generated for each isotopologue set. Quantitation using a standard curve enhances quantitation across a dynamic range of analyte.
    Type: Application
    Filed: June 6, 2014
    Publication date: December 11, 2014
    Inventors: Greg Hermanson, John Charles Rogers, Joel Louette, Ryan Daniel Bomgarden, Bhavinkumar Patel
  • METHODS AND REAGENTS FOR BIOMOLECULE LABELING, ENRICHMENT AND GENTLE ELUTION
    Publication number: 20130309689
    Abstract: Methods and sets of non-biological reagents (elution reagents, tag isomers, tag reactive groups, crosslinkers) for single or multiplexed capture and gentle elution of biomolecules. Examples are provided using amine- and cysteine-reactive reagents for enrichment of proteins, peptides, and rare peptide modifications.
    Type: Application
    Filed: March 11, 2013
    Publication date: November 21, 2013
    Applicant: Pierce Biotechnology, Inc.
    Inventors: John Charles Rogers, Ryan Daniel Bomgarden, Christopher Laurence Etienne, Eric Leigh Hommema