Abstract: Therapeutic agents such as mRNA fragments are delivered to a patient to treat peripheral nerve system injuries via functionalized fiber networks. The fiber networks are electrospun from poly(L-lactic acid), producing a hollow conduit of aligned fibers. The networks are functionalized with an anionic layer, e.g., dextran sulfate sodium salt (DSS), poly(3,4-dihydroxy-L-phenylalanine) (pDOPA). mRNA complexes including a cationic liposomal transfection portion and an mRNA portion, such as ?-modified mRNA encoding neurotrophin-3 (NT-3), are immobilized thereon. The fiber networks are positioned to bridge a peripheral nerve gap in the patient. These networks upregulate secretion of expressed oligopeptides, e.g., NT-3, by surrounding cells, and thus enhance neurite outgrowth. The networks serve as versatile non-viral mRNA delivery platforms.

Abstract: The invention generally relates to a method of regenerating a nerve fiber in a damaged neural tissue of a patient, the method comprising the steps of: administering an aqueous formulation comprising superparamagnetic particles to the damaged neural tissue in the patient; applying a magnetic field in an orientation which is parallel to the nerve fiber; using the magnetic field for aligning the superparamagnetic particles; forming one or more aligned chains of the superparamagnetic particles in the magnetic field as a scaffold to guide directional growth of regenerating nerve cells; and reconnecting damaged nerve ends in the damaged neural tissue of the patient.

Abstract: A system for automatic determination of eligibility, payments and tax for a merchandise lease comprises an interface and a processor. The interface is configured to receive a minimal set of information. The processor is configured to determine that it is possible to determine a full identity using the minimal set of information; determine a maximum lease amount based at least in part on the full identity; and determine one or more lease payments and lease tax based at least in part on a merchandise price and a location tax rate.

Abstract: A system for collection comprises a processor and a memory. The processor is configured to determine that a collector is willing to assume a financial obligation for a collectee and to determine whether the collectee has funds to repay the obligation at a financial institution. The processor is further configured to transfer the funds from the financial institution associated with the collector for repayment of the financial obligation in the event that the collectee has funds to repay the obligation at the financial institution. A memory coupled to the processor and configured to provide the processor with instructions.