Abstract: Disclosed is a device and method for a microfluidic paper-based analytical device (?PAD), for low-cost and user-friendly analytical devices capable of use for disease screening, point-of-care pathogen and biomarker detection, food and water quality testing. A microfluidic paper-based analytical device is further produced by chemical vapor deposition for multiplex heavy metal detection in water. Assay demonstrations proved that the immobilization of functional groups and multiplex heavy metal detection is suitable for real-world applications and established the approach for DNA analysis. The disclosed invention comprises multilayer capability, including the ability for various biomolecules to be immobilized with charge interaction.

Abstract: Disclosed is a device and method for a microfluidic paper-based analytical device (?PAD), for low-cost and user-friendly analytical devices capable of use for disease screening, point-of-care pathogen and biomarker detection, food and water quality testing. A microfluidic paper-based analytical device is further produced by chemical vapor deposition for multiplex heavy metal detection in water. Assay demonstrations proved that the immobilization of functional groups and multiplex heavy metal detection is suitable for real-world applications and established the approach for DNA analysis. The disclosed invention comprises multilayer capability, including the ability for various biomolecules to be immobilized with charge interaction.

Abstract: Disclosed is a device and method for forming a chemically patterned paper microfluidic device (cPMD) having controllable hydrophobic regions for purposes of providing a repeatable and versatile production with no temperature limitations similar or expensive printers, enabling point of care sensor devices. The disclosed invention comprises multilayer capability, including the ability for various biomolecules to be immobilized with charge interaction. The paper-based microfluidic platform as disclosed repeatable, versatile, cost effective, and allows for the creation of complex channels using the settling time calculated from calibration results. The disclosed system supports a wide variety of scenarios for testing, diagnostics and drug delivery, and related products and services.