Abstract: A disinfectant composition is provided that has been found to be particularly effective in inactivating and/or destroying microorganisms. The disinfectant composition can include hydrogen peroxide, an organic solvent, at least one fatty acid having 6 to 9 carbon atoms or an aromatic carboxylic acid, at least two surfactants with the first surfactant being an amphoteric surfactant and the second surfactant being a nonionic surfactant, and an acidic pH adjuster. The disinfectant composition has been found to be particularly effective against microorganisms when the organic solvent and the fatty acid or aromatic carboxylic acid are present in a ratio from about 3:1 to about 30:1, the fatty acid or aromatic carboxylic acid and the amphoteric surfactant are present in a ratio from about 0.1:1 to about 3:1, and the organic solvent and the amphoteric surfactant are present in a ratio from about 1:1 to about 30:1.