Abstract: Presented herein are techniques for indexing of nucleic acid, e.g., for use in conjunction with sequencing. The techniques include generating indexed nucleic acid fragments from an individual sample, whereby the index sequence incorporated into each index site of the nucleic acid fragment is selected from a plurality of distinguishable of index sequences and such that the population of generated nucleic acid fragments represents each index sequence from the plurality. In this manner, the generated indexed nucleic acid fragments from a single sample are indexed with a diverse mix of index sequences that reduce misassignment due to index read errors associated with low sequence diversity.

Abstract: Presented herein are techniques for indexing of nucleic acid, e.g., for use in conjunction with sequencing. The techniques include generating indexed nucleic acid fragments from an individual sample, whereby the index sequence incorporated into each index site of the nucleic acid fragment is selected from a plurality of distinguishable of index sequences and such that the population of generated nucleic acid fragments represents each index sequence from the plurality. In this manner, the generated indexed nucleic acid fragments from a single sample are indexed with a diverse mix of index sequences that reduce misassignment due to index read errors associated with low sequence diversity.

Abstract: An embodiment of a heat exchanger assembly includes a first manifold adapted for receiving a first medium, a core adapted for receiving and placing a plurality of mediums, including the first medium, in at least one heat exchange relationship, and a core meeting the first manifold at a first core/manifold interface; The mounting structure supports a heat exchanger, and is metallurgically joined to at least one heat exchanger assembly component at a first joint integrally formed with the mounting structure.

Abstract: Disclosed herein are systems and methods for multiplex primer design and selection. In one example, a system includes non-transitory memory configured to store executable instructions; and a hardware processor programmed by the executable instructions to receive a plurality of target gene sequences and determine a set of primers for each target gene sequence based on a penalty score associated with the set of primers, wherein the penalty score is based on a non-linear combination of a primer-level penalty score and a set-level penalty score.

Abstract: An embodiment of a heat exchanger assembly includes a first manifold adapted for receiving a first medium, a core adapted for receiving and placing a plurality of mediums, including the first medium, in at least one heat exchange relationship, and a core meeting the first manifold at a first core/manifold interface; The mounting structure supports a heat exchanger, and is metallurgically joined to at least one heat exchanger assembly component at a first joint integrally formed with the mounting structure.

Abstract: Computer methods and systems for quantifying a nucleic acid sample comprising nucleic acid of one or more contributors to: receive nucleic acid sequence reads obtained from the nucleic acid sample and mapped to alleles at polymorphism loci; determine, using the nucleic acid sequence reads, allele counts for each of the alleles at the polymorphism loci; use a probabilistic mixture model that applies a probabilistic mixture model to the allele counts, and that uses probability distributions to model the allele counts at the polymorphism loci; quantify, using the probabilistic mixture model, one or more fractions of nucleic acid of the one or more contributors in the nucleic acid sample; determine a probability that a specific contributor among the one or more contributors has a specific genotype; and call, based on the posterior probability, that the nucleic acid sample includes nucleic acid from the specific contributor.

Abstract: An embodiment of a heat exchanger assembly includes a first manifold adapted for receiving a first medium, a core adapted for receiving and placing a plurality of mediums, including the first medium, in at least one heat exchange relationship, and a core meeting the first manifold at a first core/manifold interface; The mounting structure supports a heat exchanger, and is metallurgically joined to at least one heat exchanger assembly component at a first joint integrally formed with the mounting structure.

Abstract: A counter-flow heat exchanger core includes a first wall defining a longitudinal axis. The first flow path is defined within the first wall. The first flow path includes a primary flow inlet and a primary flow outlet downstream from the primary flow inlet. The heat exchanger core includes at least two hollow vanes circumferentially spaced apart and extending in a radially inward direction from the first wall. Each of the at least two hollow vanes includes a first vane wall and a second vane wall. The heat exchanger core includes a second flow path defined within the at least two hollow vanes between the first vane wall and second vane wall of each of the at least two hollow vanes. The heat exchanger core includes at least one fin extending between two of the at least two circumferentially spaced apart vanes.

Abstract: A seat pan adjustment system for an ejection seat may comprise a guide bracket defining a channel and a seat pan mount configured to translate relative to the guide bracket. A support bracket may extend from a surface of the seat pan mount. A rod may be located through a rod opening defined by the support bracket. The rod may be located in the channel. A biasing member may be coupled to the rod and configured to force the rod away from the surface of the seat pan mount.

Abstract: A seat pan adjustment system for an ejection seat may comprise a guide bracket defining a channel and a seat pan mount configured to translate relative to the guide bracket. A support bracket may extend from a surface of the seat pan mount. A rod may be located through a rod opening defined by the support bracket. The rod may be located in the channel. A biasing member may be coupled to the rod and configured to force the rod away from the surface of the seat pan mount.

Abstract: Methods and systems are provided for quantifying and deconvolving nucleic acid mixture samples including nucleic acid of one or more contributors having known or unknown genomes. The methods and systems provided herein implement processes that use Bayesian probabilistic modeling techniques to determine the abundances and confidence intervals of genetically distinct contributors in a chimerism sample, thereby improving specificity, accuracy and sensitivity, and greatly expanded the application scope over conventional methods.

Abstract: Disclosed herein are systems and methods for multiplex primer design and selection. In one example, a system includes non-transitory memory configured to store executable instructions; and a hardware processor programmed by the executable instructions to receive a plurality of target gene sequences and determine a set of primers for each target gene sequence based on a penalty score associated with the set of primers, wherein the penalty score is based on a non-linear combination of a primer-level penalty score and a set-level penalty score.

Abstract: A clamp assembly includes a first arm, a second arm, a hinge, and a fastening arrangement. The first arm and the second arm each include a hinge end, a free end, a web between the hinge end and the free end, a curved portion between the hinge end and the web, and a curved portion between the free end and the web. The hinge pivotably connects the hinge ends. The fastening arrangement is configured to connect the free ends when the clamp assembly is in a closed position. The curved portions are configured to encircle a first structure and a second structure when the clamp assembly is in the closed position.

Abstract: Provided is a computer-implemented method of aligning RNA including receiving onto a data storage unit primer sequences and transcript sequences transcribable from a reference genome based on a gene model, generating target sequences to be amplified from a combination of the primer sequences and the transcript sequences, generating a modified reference genome based on the plurality of target sequences, aligning sequence reads generated from a test sample comprising RNA amplicon molecules to the of target sequences, and generating an alignment profile for the test sample based on the aligning. Also provided is a computer system for performing the foregoing method.

Abstract: An embodiment of a heat exchanger according to the disclosure includes a core configured to receive and place a plurality of mediums in at least one heat exchange relationship, and a first manifold connected to and in fluid communication with the core at a first manifold/core interface. The first manifold includes a first end distal from the core with at least one port adapted to receive or discharge a first medium of the plurality of mediums, and a second end joined to the core at the first manifold/core interface adapted to transfer the first medium to or from a plurality of first heat exchange passages in the core. A plurality of first guide vanes in the manifold defining individual layers for the first medium, and a plurality of second guide vanes divide ones of the individual layers into a plurality of first discrete manifold flow passages extending at least part of a distance from the first end to the second end of the first manifold.

Abstract: An embodiment of a heat exchanger according to the disclosure includes a core configured to receive and place a plurality of mediums in at least one heat exchange relationship, and a first manifold connected to and in fluid communication with the core at a first manifold/core interface. The first manifold includes a first end distal from the core with at least one port adapted to receive or discharge a first medium of the plurality of mediums, and a second end joined to the core at the first manifold/core interface adapted to transfer the first medium to or from a plurality of first heat exchange passages in the core. A plurality of first guide vanes in the manifold defining individual layers for the first medium, and a plurality of second guide vanes divide ones of the individual layers into a plurality of first discrete manifold flow passages extending at least part of a distance from the first end to the second end of the first manifold.

Abstract: A clamp assembly includes a first arm, a second arm, a hinge, and a fastening arrangement. The first arm and the second arm each include a hinge end, a free end, a web between the hinge end and the free end, a curved portion between the hinge end and the web, and a curved portion between the free end and the web. The hinge pivotably connects the hinge ends. The fastening arrangement is configured to connect the free ends when the clamp assembly is in a closed position. The curved portions are configured to encircle a first structure and a second structure when the clamp assembly is in the closed position.

Abstract: An embodiment of a heat exchanger assembly includes a first manifold adapted for receiving a first medium, a core adapted for receiving and placing a plurality of mediums, including the first medium, in at least one heat exchange relationship, and a core meeting the first manifold at a first core/manifold interface; The mounting structure supports a heat exchanger, and is metallurgically joined to at least one heat exchanger assembly component at a first joint integrally formed with the mounting structure.

Abstract: An embodiment of a heat exchanger core includes a plurality of walls defining a plurality of layers in at least one heat exchange relationship. At least one of the layers of the core having a first load-bearing portion aligned with and adjacent to a first mount location on a perimeter of the core, and a first non-load-bearing portion distal from the non-load-bearing portion. A topology of the first load-bearing portion has a load bearing capacity greater than a load bearing capacity of the non-load-bearing portion.

Abstract: Presented herein are techniques for indexing of nucleic acid, e.g., for use in conjunction with sequencing. The techniques include generating indexed nucleic acid fragments from an individual sample, whereby the index sequence incorporated into each index site of the nucleic acid fragment is selected from a plurality of distinguishable of index sequences and such that the population of generated nucleic acid fragments represents each index sequence from the plurality. In this manner, the generated indexed nucleic acid fragments from a single sample are indexed with a diverse mix of index sequences that reduce misassignment due to index read errors associated with low sequence diversity.