Patents by Inventor Ryan Mulqueen

Ryan Mulqueen has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20220340966
    Abstract: Disclosed are methods of targeted sequencing by using gRNA-endonuclease complexes and methods of designing pools of gRNAs. The disclosure also provides sequencing adapters that that comprise a double-stranded nucleic acid having a single-stranded overhang with degenerate overhanging bases. In a first aspect, methods for Dephosphorylate targeted sequencing of double-stranded nucleic acids comprises cleaving dephosphorylated double-stranded nucleic acids with a plurality of endonuclease-guide ribonucleic acid (gRNA) complexes to generate double-stranded nucleic acid fragments having phosphorylated 5? end overhangs at targeted sites.
    Type: Application
    Filed: September 9, 2020
    Publication date: October 27, 2022
    Inventors: Brian J. O'ROAK, Andrew ADEY, Taylor MIGHELL, Ryan MULQUEEN, Casey THORNTON
  • Publication number: 20210380972
    Abstract: The present disclosure is concerned with compositions, methods, and kits for preparing a sequencing library. In one embodiment, methods include producing a library of target nucleic acids having the same adapter at each end and then switching the identity of one adapter to result in target nucleic acids flanked by distinct adapters.
    Type: Application
    Filed: June 10, 2021
    Publication date: December 9, 2021
    Applicants: Illumina, Inc., Illumina Cambridge Limited, Oregon Health & Science University
    Inventors: Andrew C. Adey, Ryan Mulqueen, Frank Steemers, Dmitry K Pokholok, Fan Zhang, Esther Musgrave-Brown
  • Publication number: 20180355348
    Abstract: Provided herein are methods for preparing sequencing libraries for determining the methylation status of nucleic acids from a plurality of single cells. The present methods combine split-and-pool combinatorial indexing and bisulfite treatment techniques to characterize the methylation profiles of large numbers of single cells quickly, accurately and inexpensively.
    Type: Application
    Filed: June 5, 2018
    Publication date: December 13, 2018
    Inventors: Andrew C. Adey, Ryan Mulqueen, Frank J. Steemers, Dmitry K. Pokholok, Steven Norberg