Patents by Inventor Ryan RISKOWSKI

Ryan RISKOWSKI has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Metal-reducing enzymatic tag for optical and electron microscopy
    Patent number: 12339286
    Abstract: Enzymes that reduce specific metal or metalloid ions to insoluble form are important to science. Peptides isolated from yeast- and phage-display libraries can affect the size and morphology of inorganic materials during their synthesis. Herein, an Se binding peptide was fused to an enzyme capable of reducing selenite (SeO32?) to a Se0 nanoparticle (SeNP). The fusion of the Se binding peptide to the metalloid reductase provided size control of the resulting SeNP. The SeNP product also remains associated to the enzyme fusion. The Se binding peptide fusion to the enzyme increases the enzyme's SeO32? reductase activity. Modification of enzyme activity was absent, and the size control of particles was diminished when the Se binding peptide was added exogenously to the reaction mixture. Binding of the peptide is attributed to His based ligation that results in a conformational change to the peptide.
    Type: Grant
    Filed: August 9, 2019
    Date of Patent: June 24, 2025
    Assignee: COLORADO STATE UNIVERSITY RESEARCH FOUNDATION
    Inventors: Christopher J. Ackerson, Zachary Butz, Richard Nemeth, Ryan Riskowski, Kanda Borgognoni
  • A METAL-REDUCING ENZYMATIC TAG FOR OPTICAL AND ELECTRON MICROSCOPY
    Publication number: 20210311066
    Abstract: Enzymes that reduce specific metal or metalloid ions to insoluble form are important to science. Peptides isolated from yeast- and phage-display libraries can affect the size and morphology of inorganic materials during their synthesis. Herein, an Se binding peptide was fused to an enzyme capable of reducing selenite (SeO32?) to a Se0 nanoparticle (SeNP). The fusion of the Se binding peptide to the metalloid reductase provided size control of the resulting SeNP. The SeNP product also remains associated to the enzyme fusion. The Se binding peptide fusion to the enzyme increases the enzyme's SeO32? reductase activity. Modification of enzyme activity was absent, and the size control of particles was diminished when the Se binding peptide was added exogenously to the reaction mixture. Binding of the peptide is attributed to His based ligation that results in a conformational change to the peptide.
    Type: Application
    Filed: August 9, 2019
    Publication date: October 7, 2021
    Applicant: Colorado State University Research Foundation
    Inventors: Christopher J. ACKERSON, Zachary BUTZ, Richard NEMETH, Ryan RISKOWSKI, Kanda BORGOGNONI