Abstract: A method and system for interrogating a target for one or more chemical species of interest using Raman microscopy and spectroscopy. A feature includes the ability to precisely electro-mechanically move and orient a Raman microscope relative to the target with multiple degrees of freedom of movement, including targets with 3-D form factors. This promotes effective detection of minute quantities of chemical species of interest. It also allows effective detection of minute quantities whether the target is static or moving. The method and system can include enhancements. Examples include alternative imaging spectrometers, alternative Raman microscope optical set-ups, and alternative focusing techniques. Others include control of the excitation energy and user controls and options to allow highly adjustable, flexible, and effective detection for a variety of detection applications.