Patents by Inventor Ryan Sillers
Ryan Sillers has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11873520Abstract: The present invention provides for novel metabolic pathways to convert biomass and other carbohydrate sources to malonyl-CoA derived products, such as hydrocarbons and other bioproducts, under anaerobic conditions and with the net production of ATP. More specifically, the invention provides for a recombinant microorganism comprising one or more native and/or heterologous enzymes that function in one or more engineered metabolic pathways to achieve conversion of a carbohydrate source to, e.g., long-chain hydrocarbons and hydrocarbon derivatives, wherein the one or more native and/or heterologous enzymes is activated, upregulated, downregulated, or deleted. The invention also provides for processes to convert biomass to malonyl-CoA derived products which comprise contacting a carbohydrate source with a recombinant microorganism of the invention.Type: GrantFiled: September 30, 2021Date of Patent: January 16, 2024Assignee: Lallemand Hungary Liquidity Management LLCInventors: William Ryan Sillers, Shital A. Tripathi, Arthur J. Shaw, IV, Aaron Argyros, David A. Hogsett
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Patent number: 11685938Abstract: The subject of this invention is improvements in the yield and titer of biological production of muconic acid by fermentation. Increased activity of one or more enzymes involved in the muconic acid pathway leads to increased production of muconic acid.Type: GrantFiled: May 21, 2021Date of Patent: June 27, 2023Assignee: PTT GLOBAL CHEMICAL PUBLIC COMPANY LIMITEDInventors: Ryan Sillers, Theron Hermann, Michelle Spencer, Russell Udani, R. Rogers Yocum
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Patent number: 11634735Abstract: The present invention provides for novel metabolic pathways leading to propanol, alcohol or polyol formation in a consolidated bioprocessing system (CBP), where lignocellulosic biomass is efficiently converted to such products. More specifically, the invention provides for a recombinant microorganism, where the microorganism expresses one or more native and/or heterologous enzymes; where the one or more enzymes function in one or more engineered metabolic pathways to achieve: (1) conversion of a carbohydrate source to 1,2-propanediol, isopropropanol, ethanol and/or glycerol; (2) conversion of a carbohydrate source to n-propanol and isopropanol; (3) conversion of a carbohydrate source to isopropanol and methanol; or (4) conversion of a carbohydrate source to propanediol and acetone; wherein the one or more native and/or heterologous enzymes is activated, upregulated or downregulated.Type: GrantFiled: July 1, 2020Date of Patent: April 25, 2023Assignee: Lallemand Hungary Liquidity Management LLCInventors: John E. McBride, Vineet Rajgarhia, Arthur J. Shaw, IV, Shital A. Tripathi, Elena Brevnova, Nicky Caiazza, Johannes Pieter Van Dijken, Allan C. Froehlich, William Ryan Sillers, James H. Flatt
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Publication number: 20220267816Abstract: The present invention provides for novel metabolic pathways to convert biomass and other carbohydrate sources to malonyl-CoA derived products, such as hydrocarbons and other bioproducts, under anaerobic conditions and with the net production of ATP. More specifically, the invention provides for a recombinant microorganism comprising one or more native and/or heterologous enzymes that function in one or more engineered metabolic pathways to achieve conversion of a carbohydrate source to, e.g., long-chain hydrocarbons and hydrocarbon derivatives, wherein the one or more native and/or heterologous enzymes is activated, upregulated, downregulated, or deleted. The invention also provides for processes to convert biomass to malonyl-CoA derived products which comprise contacting a carbohydrate source with a recombinant microorganism of the invention.Type: ApplicationFiled: September 30, 2021Publication date: August 25, 2022Inventors: William Ryan Sillers, Shital A. Tripathi, Arthur J. Shaw, IV, Aaron Argyros, David A. Hogsett
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Publication number: 20220098600Abstract: The present invention provides for novel metabolic pathways to reduce or eliminate glycerol production and increase product formation. More specifically, the invention provides for a recombinant microorganism comprising a deletion of one or more native enzymes that function to produce glycerol and/or regulate glycerol synthesis and one or more native and/or heterologous enzymes that function in one or more engineered metabolic pathways to convert a carbohydrate source, such as lignocellulose, to a product, such as ethanol, wherein the one or more native and/or heterologous enzymes is activated, upregulated, or downregulated.Type: ApplicationFiled: May 10, 2021Publication date: March 31, 2022Inventors: Aaron Argyros, William Ryan Sillers, Trisha Barrett, Nicky Caiazza, Arthur J. Shaw, IV
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Publication number: 20220090149Abstract: The present invention discloses a genetically engineered Kluyveromyces sp. yeast strain that is capable of producing lactic acid from carbon source selected from glucose, fructose, sucrose or a mixture thereof wherein the genetically engineered yeast comprises at least one heterologous DNA cassette that confers production of a protein functioning as a fructose importer. The genetically engineered yeast strain according to this invention has an improvement of fructose utilization and use fructose as a faster rate than conventional strain, allowing for shorter fermentation times and improved economics.Type: ApplicationFiled: December 23, 2019Publication date: March 24, 2022Applicant: PTT GLOBAL CHEMICAL PUBLIC COMPANY LIMITEDInventors: Sudhanshu Vijay DOLE, Joel Stewart SCHMID, R. Rogers YOCUM, Theron HERMANN, Russell Lizardo UDANI, Sean Joseph REGAN, Mark Andrew SHEFF, Michelle SPENCER, Ryan SILLERS, Phatthanon PRASITCHOKE, Natthawut POOMSILA
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Patent number: 11162125Abstract: The present invention provides for novel metabolic pathways to convert biomass and other carbohydrate sources to malonyl-CoA derived products, such as hydrocarbons and other bioproducts, under anaerobic conditions and with the net production of ATP. More specifically, the invention provides for a recombinant microorganism comprising one or more native and/or heterologous enzymes that function in one or more engineered metabolic pathways to achieve conversion of a carbohydrate source to, e.g., long-chain hydrocarbons and hydrocarbon derivatives, wherein the one or more native and/or heterologous enzymes is activated, upregulated, downregulated, or deleted. The invention also provides for processes to convert biomass to malonyl-CoA derived products which comprise contacting a carbohydrate source with a recombinant microorganism of the invention.Type: GrantFiled: October 22, 2018Date of Patent: November 2, 2021Assignee: Lallemand Hungary Liquidity Management LLCInventors: William Ryan Sillers, Shital A. Tripathi, Arthur J. Shaw, IV, Aaron Argyros, David A. Hogsett
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Publication number: 20210277429Abstract: The subject of this invention is improvements in the yield and titer of biological production of muconic acid by fermentation. Increased activity of one or more enzymes involved in the muconic acid pathway leads to increased production of muconic acid.Type: ApplicationFiled: May 21, 2021Publication date: September 9, 2021Applicant: PTT GLOBAL CHEMICAL PUBLIC COMPANY LIMITEDInventors: Ryan SILLERS, Theron HERMANN, Michelle SPENCER, Russell UDANI, R. Rogers YOCUM
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Patent number: 11034982Abstract: Improved yeast cell with increased succinic acid production based on yield and titer. Increased activity of one or more enzymes involved in the pentose phosphate pathway, reducing flux through phosphoglucose isomerase, increasing flux to cytoplasmic acetyl-CoA, installation of a malic enzyme, and/or installation of a formate dehydrogenase leads to increased production of succinic acid.Type: GrantFiled: May 23, 2018Date of Patent: June 15, 2021Assignee: PTT GLOBAL CHEMICAL PUBLIC COMPANY LIMITEDInventor: Ryan Sillers
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Patent number: 11034967Abstract: The present invention provides for novel metabolic pathways to reduce or eliminate glycerol production and increase product formation. More specifically, the invention provides for a recombinant microorganism comprising a deletion of one or more native enzymes that function to produce glycerol and/or regulate glycerol synthesis and one or more native and/or heterologous enzymes that function in one or more engineered metabolic pathways to convert a carbohydrate source, such as lignocellulose, to a product, such as ethanol, wherein the one or more native and/or heterologous enzymes is activated, upregulated, or downregulated.Type: GrantFiled: June 30, 2017Date of Patent: June 15, 2021Assignee: Lallemand Hungary Liquidity Management LLCInventors: Aaron Argyros, William Ryan Sillers, Trisha Barrett, Nicky Caiazza, Arthur J. Shaw, IV
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Publication number: 20200325500Abstract: The present invention provides for novel metabolic pathways leading to propanol, alcohol or polyol formation in a consolidated bioprocessing system (CBP), where lignocellulosic biomass is efficiently converted to such products. More specifically, the invention provides for a recombinant microorganism, where the microorganism expresses one or more native and/or heterologous enzymes; where the one or more enzymes function in one or more engineered metabolic pathways to achieve: (1) conversion of a carbohydrate source to 1,2-propanediol, isopropropanol, ethanol and/or glycerol; (2) conversion of a carbohydrate source to n-propanol and isopropanol; (3) conversion of a carbohydrate source to isopropanol and methanol; or (4) conversion of a carbohydrate source to propanediol and acetone; wherein the one or more native and/or heterologous enzymes is activated, upregulated or downregulated.Type: ApplicationFiled: July 1, 2020Publication date: October 15, 2020Inventors: John E. McBride, Vineet Rajgarhia, Arthur J. Shaw, IV, Shital A. Tripathi, Elena Brevnova, Nicky Caiazza, Johannes Pieter Van Dijken, Allan C. Froehlich, William Ryan Sillers, James H. Flatt
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Publication number: 20200270657Abstract: The present invention provides for novel metabolic pathways to convert biomass and other carbohydrate sources to malonyl-CoA derived products, such as hydrocarbons and other bioproducts, under anaerobic conditions and with the net production of ATP. More specifically, the invention provides for a recombinant microorganism comprising one or more native and/or heterologous enzymes that function in one or more engineered metabolic pathways to achieve conversion of a carbohydrate source to, e.g., long-chain hydrocarbons and hydrocarbon derivatives, wherein the one or more native and/or heterologous enzymes is activated, upregulated, downregulated, or deleted. The invention also provides for processes to convert biomass to malonyl-CoA derived products which comprise contacting a carbohydrate source with a recombinant microorganism of the invention.Type: ApplicationFiled: October 22, 2018Publication date: August 27, 2020Inventors: William Ryan Sillers, Shital A. Tripathi, Arthur J. Shaw, IV, Aaron Argyros, David A. Hogsett
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Publication number: 20200181658Abstract: The subject of this invention is improvements in the yield and titer of biological production of muconic acid by fermentation. Increased activity of one or more enzymes involved in the muconic acid pathway leads to increased production of muconic acid.Type: ApplicationFiled: March 1, 2017Publication date: June 11, 2020Applicant: PTT Global Chemical Public Company LimitedInventors: Ryan Sillers, Theron Hermann, Michelle Spencer, Russell Udani, R. Rogers Yocum
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Publication number: 20200157583Abstract: Improved yeast cell with increased succinic acid production based on yield and titer. Increased activity of one or more enzymes involved in the pentose phosphate pathway, reducing flux through phosphoglucose isomerase, increasing flux to cytoplasmic acetyl-CoA, installation of a malic enzyme, and/or installation of a formate dehydrogenase leads to increased production of succinic acid.Type: ApplicationFiled: May 23, 2018Publication date: May 21, 2020Applicant: PTT GLOBAL CHEMICAL PUBLIC COMPANY LIMITEDInventor: Ryan SILLERS
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Publication number: 20200131538Abstract: The invention provides processes for identifying and tracking genomic duplications that can occur during classical strain development or during the metabolic evolution of microbial strains originally constructed for the production of a biochemical through specific genetic manipulations, processes that stabilize the copy number of desirable genomic duplications using appropriate selectable markers, and non-naturally occurring microorganisms with stabilized copy numbers of a functional DNA sequence.Type: ApplicationFiled: June 29, 2018Publication date: April 30, 2020Applicant: PTT GLOBAL CHEMICAL PUBLIC COMPANY LIMITEDInventors: R. Rogers YOCUM, Tammy GRABAR, Theron HERMANN, Christopher Joseph MARTIN, Ryan SILLERS, Xiaohui YU, Xiaomei ZHOU
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Publication number: 20190345437Abstract: This present invention is in the field of producing renewable chemical feedstocks using biocatalysts that have been genetically engineered to increase their ability to convert renewable carbon resources into useful compounds. More specifically, the present invention provides a process for producing muconic acid form renewable carbon resources using a genetically modified organism.Type: ApplicationFiled: July 10, 2019Publication date: November 14, 2019Applicant: PTTGC INNOVATION AMERICA CORPORATIONInventors: R. Rogers YOCUM, Wei GONG, Sudhanshu DOLE, Ryan SILLERS, Meghal GANDHI, Janice G. PERO
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Patent number: 10138504Abstract: The present invention provides for novel metabolic pathways to convert biomass and other carbohydrate sources to malonyl-CoA derived products, such as hydrocarbons and other bioproducts, under anaerobic conditions and with the net production of ATP. More specifically, the invention provides for a recombinant microorganism comprising one or more native and/or heterologous enzymes that function in one or more engineered metabolic pathways to achieve conversion of a carbohydrate source to, e.g., long-chain hydrocarbons and hydrocarbon derivatives, wherein the one or more native and/or heterologous enzymes is activated, upregulated, downregulated, or deleted. The invention also provides for processes to convert biomass to malonyl-CoA derived products which comprise contacting a carbohydrate source with a recombinant microorganism of the invention.Type: GrantFiled: August 5, 2011Date of Patent: November 27, 2018Assignee: Lallemand Hungary Liquidity Management LLCInventors: William Ryan Sillers, Shital A. Tripathi, Arthur J. Shaw, IV, Aaron Argyros, David A. Hogsett
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Publication number: 20180208951Abstract: The present in provides for novel metabolic pathways leading to propanol, alcohol or polyol formation in a consolidated bioprocessing system (CBP), where lignocellulosic biomass is efficiently converted to such products. More specifically, the invention provides for a recombinant microorganism, where the microorganism expresses one or more native and/or heterologous enzymes; where the one or more enzymes function in one or more engineered metabolic pathways to achieve: (1) conversion of a carbohydrate source to 1,2-propanediol, isopropropanol, ethanol and/or glycerol; (2) conversion of a carbohydrate source to n-propanol and isopropanol; (3) conversion of a carbohydrate source to isopropanol and methanol; or (4) conversion of a carbohydrate source to propanediol and acetone; wherein the one or more native and/or heterologous enzymes is activated, upregulated or downregulated.Type: ApplicationFiled: March 21, 2018Publication date: July 26, 2018Inventors: John E. McBride, Vineet Rajgarhia, Arthur J. Shaw, IV, Shital A. Tripathi, Elena Brevnova, Nicky Caiazza, Johannes Pieter Van Dijken, Allan C. Froehlich, William Ryan Sillers, James H. Flatt
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Patent number: 9957530Abstract: The present invention provides for novel metabolic pathways leading to propanol, alcohol or polyol formation in a consolidated bioprocessing system (CBP), where lignocellulosic biomass is efficiently converted to such products. More specifically, the invention provides for a recombinant microorganism, where the microorganism expresses one or more native and/or heterologous enzymes; where the one or more enzymes function in one or more engineered metabolic pathways to achieve: (1) conversion of a carbohydrate source to 1,2-propanediol, isopropropanol, ethanol and/or glycerol; (2) conversion of a carbohydrate source to n-propanol and isopropanol; (3) conversion of a carbohydrate source to isopropanol and methanol; or (4) conversion of a carbohydrate source to propanediol and acetone; wherein the one or more native and/or heterologous enzymes is activated, upregulated or downregulated.Type: GrantFiled: August 20, 2010Date of Patent: May 1, 2018Assignee: Lallemand Hungary Liquidity Management LLCInventors: John E. McBride, Vineet Rajgarhia, Arthur J. Shaw, IV, Shital A. Tripathi, Elena Brevnova, Nicky Caiazza, Johannes Pieter Van Dijken, Allan C. Froehlich, William Ryan Sillers, James H. Flatt
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Publication number: 20170356000Abstract: The present invention provides for novel metabolic pathways to reduce or eliminate glycerol production and increase product formation. More specifically, the invention provides for a recombinant microorganism comprising a deletion of one or more native enzymes that function to produce glycerol and/or regulate glycerol synthesis and one or more native and/or heterologous enzymes that function in one or more engineered metabolic pathways to convert a carbohydrate source, such as lignocellulose, to a product, such as ethanol, wherein the one or more native and/or heterologous enzymes is activated, upregulated, or downregulated.Type: ApplicationFiled: June 30, 2017Publication date: December 14, 2017Inventors: Aaron Argyros, William Ryan Sillers, Trisha Barrett, Nicky Caiazza, Arthur J. Shaw, IV