Abstract: Systems and methods are disclosed for a shuttle sortation system having a calibration shuttle for determining and adjusting shuttle coordinates for depositing items and/or loading totes at various tote receiving areas. The shuttle sortation system may include item tracks and tote tracks for sorting and/or distributing items, packages, and/or totes, and may move the shuttles about the shuttle sortation system via linear synchronous motors (LSMs). The calibration shuttle may include a base portion designed to interface with the track (e.g., with the LSMs). The base may support imaging systems having cameras and processors for capturing images of the tote receiving areas and processing the images to determine a centerline of such tote receiving areas and adjusting coordinates used to navigate the shuttle to the tote receiving areas. The calibration shuttle may be navigated to each tote receiving area to adjust coordinates where an offset from the centerline is detected.