Abstract: A molten iron refining method having, an auxiliary material, and an oxidizing gas supplied through a top-blowing lance, to a cold iron source and molten pig iron that are contained/fed in a converter-type vessel, and molten iron is subjected to a refining process. A pre-charged cold iron source is charged into the converter-type vessel at an amount not larger than 0.15 times. A furnace-top-added cold iron source that's part or all of the cold iron source and added from a furnace top is fed during the refining process. A burner at a leading end of the top-blowing lance that spray holes through which a fuel and a combustion-supporting gas are ejected. During the refining process, a powdery auxiliary material processed into powder that's part of the auxiliary material is blown in, to pass through a flame formed by the burner.

Abstract: A cell isolation method includes: a cell trapping step of allowing a test liquid to pass through a cell trapping filter which has a plurality of through-holes in the thickness direction, thereby trapping isolation target cells contained in the test liquid on one surface of the cell trapping filter; a gel embedding step of introducing a stimulus-responsive hydrogel onto the one surface of the cell trapping filter on which the cells have been trapped in the cell trapping step, thereby embedding the cells in the stimulus-responsive hydrogel; a gel hardening step of applying a stimulus to the stimulus-responsive hydrogel in which the cells are embedded, thereby hardening the stimulus-responsive hydrogel; and a detachment step of detaching the stimulus-responsive hydrogel that was hardened in the gel hardening step from the cell trapping filter.

Abstract: A cell isolation method includes: a cell trapping step of allowing a test liquid to pass through a cell trapping filter which has a plurality of through-holes in the thickness direction, thereby trapping isolation target cells contained in the test liquid on one surface of the cell trapping filter; a gel embedding step of introducing a stimulus-responsive hydrogel onto the one surface of the cell trapping filter on which the cells have been trapped in the cell trapping step, thereby embedding the cells in the stimulus-responsive hydrogel; a gel hardening step of applying a stimulus to the stimulus-responsive hydrogel in which the cells are embedded, thereby hardening the stimulus-responsive hydrogel; and a detachment step of detaching the stimulus-responsive hydrogel that was hardened in the gel hardening step from the cell trapping filter.