Abstract: A device for stably maintaining the shape of a fragile object in a liquid includes a container for accommodating the fragile object, a lid member for sealing the container, and a protecting member. The device is configured to form a liquid-tight space by covering a liquid surface in the container which accommodates the fragile object and the liquid with the protecting member and further covering the protecting member and an open portion of the container with the lid member.

Abstract: A method is disclosed for modifying a sheet-shaped cell culture containing at least two types of cells. The method includes soaking the sheet-shaped cell culture in a low nutrient isotonic solution; and changing a content ratio of the at least two cell types constituting the sheet-shaped cell culture.

Abstract: [Problem] To evaluate objective cell groups by estimating a mixing ratio of objective cell group without affecting treatment itself and its production process when the objective cell groups include plural kinds of cell groups having different attributes, and to provide an image analysis system and a culture management system capable of accurately perform a quality control and a production control with low cost. [Solution] The cell quality evaluation system 1 detects a feature amount in each cell easily analyzable from images, and estimates a mixing ratio of each of plural kinds of cell groups included in the objective cell groups based on a distribution of the detected feature amount and pre-recorded information of the feature amount. As the feature amount, the embodiment uses a migration speed of each cell, easily distinguishable by analyzing tracking of each cell from plural images in time series.

Abstract: An osteotomy surgical apparatus according to the present invention includes a bone cutting blade used for cutting bone tissue and a guard disposed so as to overlap the bone cutting blade in a side view of the bone cutting blade. A distal end of the guard is disposed at a position retracted rearward relative to a blade edge of the bone cutting blade.

Abstract: A laminated foam sheet obtained by a coextrusion lamination method and having a polyethylene foam layer, and an antistatic layer laminated on at least one side of the foam layer. The antistatic layer contains a polyethylene resin, an ionomer resin and a polyalkylene glycol and has such specific morphology that the polyethylene resin forms a continuous phase and the ionomer resin forms small dispersed phases dispersed in the continuous phase.

Abstract: A three-layer structure is comprised of an extra-cellular matrix, a sheet-shaped cell culture, and a biodegradable gel layer (single layer). By virtue of this three-layer structure, breakage of the sheet-shaped cell culture due to high spray pressure is reduced. The fibrin gel may be formed on one surface of the sheet-shaped cell culture by spraying of a thrombin liquid. A production method may involve culturing cells on a temperature-responsive cell culture substrate, dripping fibrinogen onto a sheet-shaped cell culture having an extra-cellular matrix on a lower surface thereof, further spraying a thrombin liquid onto the sheet-shaped cell culture from an oblique direction, whereby the result is a three-layer structure having a fibrin gel layer only on one surface of the sheet-shaped cell culture and having an extra-cellular matrix layer on the opposite surface.

Abstract: A holding tool is configured to efficiently and reliably enable a close contact between a container and a lid with a simple mechanism and a simple operation. The holding tool is configured to be used together with a container and a lid for the container to sandwich the container and the lid. The holding tool includes a base on which the container is placed, and a cover configured to cover the lid. The base and the cover each have an opening at a central portion and are configured to engage with each other to press and sandwich the container and the lid upward and downward.

Abstract: A pressing tool for bone surgery according to the present invention includes a pressing member formed substantially columnar shape having, at one end, a pressing surface formed of a convex and substantially spherical surface; and a hook member formed to have a hook shape that is to be engaged with a surface of a bone, the hook member having a support portion at a first end, the support portion supporting the pressing member so that the pressing surface is directed toward a second end, and a projection portion at the second end, the projection portion projecting toward the supporting portion and being to be bited into the surface of the bone, wherein the support portion supports the pressing member in state of the pressing member is movable in a longitudinal direction toward the projection portion.

Abstract: A method for evaluating the ability of cells to grow into a sheet includes (1) a step of examining cell cultures growing into a sheet to determine their impedance and/or electrical resistance or numerical values relating thereto; (2) a step of comparing the numerical values determined by Step (1) with reference values; and (3) a step of judging, based on the results of comparison obtained in Step (2), the ability of cells to grow into a sheet.

Abstract: A device for transporting objects such as a graft is configured to efficiently establish a liquid-tight state with a simple operation. The device is to be used in a state of being detachably attached to a container for storing a graft, the device including: a lid member for sealing the container; and an annular member for forming a first space by being interposed between the lid member and the container, wherein a second space is formed between the lid member and an inside surface of the container.

Abstract: The bone plate of the present invention includes a body portion to be fixed to an obliquely anterior medial surface of the tibia below the osteotomy surface; a wide portion to be fixed to an outer peripheral surface of the tibia at a higher position than the osteotomy surface; a connecting portion that connects the body portion and the wide portion; and a plurality of screw holes arrayed in the body portion, the wide portion and the connecting portion. An inner surface that extends from the wide portion to the connecting portion and faces the tibia has a continuous curved shape twisted about an axis along the longitudinal direction of the tibia. The wide portion is offset in an outward plate thickness direction with respect to the body portion.

Abstract: A laminated foam sheet obtained by a coextrusion lamination method and having a polyethylene foam layer, and an antistatic layer laminated on at least one side of the foam layer. The antistatic layer contains a polyethylene resin, an ionomer resin and a polyalkylene glycol and has such specific morphology that the polyethylene resin forms a continuous phase and the ionomer resin forms small dispersed phases dispersed in the continuous phase.

Abstract: A laminated foam interleaf sheet for being interposed between glass plates, having a foam layer of a polyethylene-based resin, and an antistatic layer which is laminated on each of both sides of the foam layer and contains a polyethylene-based resin, a polystyrene-based resin and a polymeric antistatic agent, the laminated foam interleaf sheet having a surface roughness Ra of 30 ?m or less.

Abstract: A method is disclosed for recovering living cells highly efficiently from cryopreserved cells by thawing and a system designed for such a method. The method for recovering living cells from cryopreserved cells includes thawing cryopreserved cells and diluting the thawed cell suspension with a diluent, wherein the dilution is performed in such a way that the maximum load of osmotic pressure at the time of dilution is equal to or less than 250 mOsm/second.

Abstract: A device for stably maintaining the shape of a fragile object in a liquid includes a container for accommodating the fragile object, a lid member for sealing the container, and a protecting member. The device is configured to form a liquid-tight space by covering a liquid surface in the container which accommodates the fragile object and the liquid with the protecting member and further covering the protecting member and an open portion of the container with the lid member.

Abstract: A technique is disclosed for preserving a shape of a fragile object in a liquid. The technique includes a device including a container that houses a fragile object and has a cylindrical shape including a bottom, a lid that seals the container, and a seal. The container includes a lower portion that houses the fragile object, an upper portion that includes an opening, and a stepped portion provided between the lower portion and the upper portion. The lid seals the opening of the upper portion. The seal is interposed between the lid and the stepped portion to seal an opening of the lower portion.

Abstract: A multi-layer foam sheet with an apparent density of 30 to 300 kg/m3 and a thickness of 0.05 to 2 mm, including a foam layer containing a polyethylene-based resin, and an antistatic layer fusion-laminated by coextrusion on each of both sides of the foam layer. The antistatic layer has a basis weight of 1 to 10 g/m2 and contains a polyethylene-based resin, a polystyrene-based resin, a styrenic elastomer, and a polymeric antistatic agent, with the polystyrene-based resin being contained in the antistatic layer in an amount of 15 to 70% by weight.

Abstract: To provide a preservative solution for live cells or a composition containing live cells, a method for preserving live cells or a composition containing live cells by use of the preservative solution and the like, the preservative solution being easy to prepare and exhibiting a high cytoprotective effect, especially during low-temperature storage. The preservative solution for live cells or a composition containing live cells contains vitamin C and/or vitamin E or edaravone in a solution serving as a base of the preservative solution, for example, a physiological isotonic solution.

Abstract: To provide a preservative solution for live cells or a composition containing live cells, a method for preserving live cells or a composition containing live cells by use of the preservative solution and the like, the preservative solution being easy to prepare and exhibiting a high cytoprotective effect, especially during low-temperature storage. The preservative solution for live cells or a composition containing live cells contains vitamin C and/or vitamin E or edaravone in a solution serving as a base of the preservative solution, for example, a physiological isotonic solution.

Abstract: [Problem] To evaluate objective cell groups by estimating a mixing ratio of objective cell group without affecting treatment itself and its production process when the objective cell groups include plural kinds of cell groups having different attributes, and to provide an image analysis system and a culture management system capable of accurately perform a quality control and a production control with low cost. [Solution] The cell quality evaluation system 1 detects a feature amount in each cell easily analyzable from images, and estimates a mixing ratio of each of plural kinds of cell groups included in the objective cell groups based on a distribution of the detected feature amount and pre-recorded information of the feature amount. As the feature amount, the embodiment uses a migration speed of each cell, easily distinguishable by analyzing tracking of each cell from plural images in time series.