Abstract: This combustion adjustment method applied to the combustion control of a combustor executes a first step for selecting a combustion parameter for a load of a gas turbine and increasing or decreasing a command value of the combustion parameter from an original location. When the command value reaches a target tolerance upper limit value or a target tolerance lower limit value, the first step is terminated. Furthermore, a second step for decreasing or increasing the command value in a direction opposite to that of the first step is executed. When the command value reaches the target tolerance upper limit value or the target tolerance lower limit value, the present invention includes a combustion tolerance confirmation step for confirming a combustion tolerance range of the combustion parameter at which the second step is terminated.

Abstract: A control device is a device for controlling a gas turbine. The control device is provided with: a prediction unit configured to predict a future state quantity of the gas turbine corresponding to a control input to the gas turbine in a prediction horizon, using a prediction model; an optimization unit configured to optimize the control input in at least a part of the prediction horizon, using a prediction result of the prediction unit; a storage unit for storing sensitivity information indicating sensitivity of the control input to a change speed of the state quantity for each operating condition of the gas turbine; and an update unit configured to read the sensitivity information corresponding to the operating condition assumed in the prediction horizon from the storage unit, and update one or more coefficients of a prediction equation of the state quantity used in the prediction model.

Abstract: A control device is a device for controlling a gas turbine. The control device is provided with: a prediction unit configured to predict a future state quantity of the gas turbine corresponding to a control input to the gas turbine in a prediction horizon, using a prediction model; an optimization unit configured to optimize the control input in at least a part of the prediction horizon, using a prediction result of the prediction unit; a storage unit for storing sensitivity information indicating sensitivity of the control input to a change speed of the state quantity for each operating condition of the gas turbine; and an update unit configured to read the sensitivity information corresponding to the operating condition assumed in the prediction horizon from the storage unit, and update one or more coefficients of a prediction equation of the state quantity used in the prediction model.

Abstract: A fuel control device includes a combustion temperature estimation value calculation unit that calculates a temperature estimation value when a mixture of fuel and inflow air is burned using an atmospheric condition, an opening degree command value of a valve that controls the amount of air that is mixed with the fuel and burned, and an output prediction value calculated on the basis of a fuel control signal command value used for calculation of a total fuel flow rate flowing through a plurality of fuel supply systems, a fuel distribution command value calculation unit that calculates a fuel distribution command value indicating a distribution of fuel output from the fuel supply systems based on the temperature estimation value, and outputs the fuel distribution command value, and a valve opening degree calculation unit that calculates each valve opening degree of a fuel flow rate control valve of the fuel supply systems.

Abstract: A fuel control device includes a combustion temperature estimation value calculation unit that calculates a temperature estimation value when a mixture of fuel and inflow air is burned using an atmospheric condition, an opening degree command value of a valve that controls the amount of air that is mixed with the fuel and burned, and an output prediction value calculated on the basis of a fuel control signal command value used for calculation of a total fuel flow rate flowing through a plurality of fuel supply systems, a fuel distribution command value calculation unit that calculates a fuel distribution command value indicating a distribution of fuel output from the fuel supply systems based on the temperature estimation value, and outputs the fuel distribution command value, and a valve opening degree calculation unit that calculates each valve opening degree of a fuel flow rate control valve of the fuel supply systems.

Abstract: This invention provides a culture control method and a cell culture apparatus that enable stable cell culture by regulating shear stress to be applied to cells within an adequate range. With the application of such culture control method and cell culture apparatus, cell culture is performed under agitation culture conditions in which the shear stress distribution is 0.5 Pa to 20 Pa in 80% or more of the culture vessel by volume.

Abstract: A fuel control device includes a combustion temperature estimation value calculation unit that calculates a temperature estimation value when a mixture of fuel and inflow air is burned using an atmospheric condition, an opening degree command value of a valve that controls the amount of air that is mixed with the fuel and burned, and an output prediction value calculated on the basis of a fuel control signal command value used for calculation of a total fuel flow rate flowing through a plurality of fuel supply systems, a fuel distribution command value calculation unit that calculates a fuel distribution command value indicating a distribution of fuel output from the fuel supply systems based on the temperature estimation value, and outputs the fuel distribution command value, and a valve opening degree calculation unit that calculates each valve opening degree of a fuel flow rate control valve of the fuel supply systems.

Abstract: A gas turbine is provided with a combustor having a pilot nozzle, a first main nozzle, and a second main nozzle. A combustor control device has a load interruption detector which detects load interruption of the gas turbine, a pilot nozzle flow rate control unit which increases the amount of premixed fuel supplied to the pilot nozzle, based on the detection of the load interruption, a first main nozzle flow rate control unit which reduces the amount of premixed fuel supplied to the first main nozzles, based on the detection of the load interruption, and a second main nozzle flow rate control unit which reduces the amount of premixed fuel supplied to the second main nozzles to a predetermined amount, based on the detection of the load interruption, and then further reduces the amount of premixed fuel supplied after the elapse of a predetermined time.

Abstract: A method for easily and non-invasively determining a degree of stratification and/or differentiation of cultured cells, and an apparatus for determining a degree of stratification and/or differentiation of cultured cells are provided. The invention relates to a method for determining a degree of stratification and/or differentiation of cultured cells, including: (a) a harvesting step of harvesting a culture solution of the cultured cells; (b) an analytical step of analyzing at least one type of a metabolite of the pentose phosphate pathway and/or the TCA cycle in the culture solution; and (c) a determination step of referring to a database of correlations between prospectively-obtained degrees of stratification and/or differentiation of the cultured cells and analytical results of metabolites, for analytical results obtained in the analytical step, to determine the degree of stratification and/or differentiation of the cultured cells.

Abstract: This invention provides a culture control method and a cell culture apparatus that enable stable cell culture by regulating shear stress to be applied to cells within an adequate range. With the application of such culture control method and cell culture apparatus, cell culture is performed under agitation culture conditions in which the shear stress distribution is 0.5 Pa to 20 Pa in 80% or more of the culture vessel by volume.

Abstract: A state of cells while being cultivated is ascertained and a feed medium suitable for the cell state is added for producing a target substance to be produced with a high yield. The system for cultivating cells of the invention includes a bioreactor/fermentor for cultivating cells to be cultivated, a measuring device for measuring the culture cells being cultivated in the bioreactor/fermentor or a component contained in the culture solution, and a control device for selecting a feed medium to be added to the bioreactor/fermentor from two or more feed media having different composition ratios based on the culture cells state determined by measured values obtained by the measuring device.

Abstract: A cell is separated from a culture broth with an extremely simple configuration, and production yield of a target product is improved. There are provided separating means for separating a cell from the culture broth containing the cell, a buffer tank connected to the separating means through a first pipeline, at least one or more liquid reservoir tank connected to the buffer tank through a second pipeline, a first valve disposed on the first pipeline and controlling communication between the separating means and the buffer tank, and a second valve disposed on the second pipeline and controlling communication between the buffer tank and the liquid reservoir tank.

Abstract: An automatic protein quantitative measurement apparatus having a reduced burden on an analysis operator, being free from bacterial contamination in each process device during collection of a sample liquid, having reduced measurement time and being capable of downsizing is provided. The automatic protein quantitative measurement apparatus has a sampling unit for automatically collecting a sample liquid online from at least one operation process of a cell culture step (wherein an antibody or other proteins are produced and secreted into the culture medium) and a separation step or a culture product purification step in a plant for culturing biological cells to produce a protein, a preprocessing unit for performing at least one of dilution and filtration of the sample liquid to adjust the liquid composition, a reaction unit having a cross-sectional area of 0.

Abstract: In order to investigate an object, such as a culture of micro-organisms, the object is repeatedly captured at two different magnifications by a suitable image pick-up device. The images at the two different magnifications are then analysed by a suitable picture image recognition device and the results of the repeated analysis are compared, thereby to derive a measurement of the change in the object with time. Thus, for a culture of micro-organisms, the number of cells can be determined at one magnification and the number of microscopic spherical bodies can be determined at another magnification, to obtain a measure of the activity of the culture. Preferably, one or more conditions of the object are then controlled on the basis of the measurement of change.

Abstract: A centrifugal separation method for living cells is disclosed in which a cell containing solution is charged in a rotor chamber confined by a steam pressure-resistant wall. A rotor having septa is disposed in the rotor chamber. The septa are rotatable in the same direction at the same angular velocity as the rotor while at least part thereof is immersed in a solution in the rotor when cells are to be centrifuged from the cell-containing solution. After the cells are separated and adhered to the inner surface of the rotor, a supernatant in the rotor is discharged form the rotor. Then a fresh cultivating medium is introduced into the rotor, and the septa and the rotor are rotated at mutually different angular velocities while at least part of the septa is immersed in the fresh medium in the rotor. As a result, the cells are again suspended in the fresh medium.

Abstract: Cross-linked polyglucans obtained by cross-linking polyglucans having branched chains intermolecularly and/or intramolecularly or cross-linked homooligomers obtained by cross-linking .alpha.-1,4-linked homooligomers of glucose intermolecularly can selectively adsorb glucoamylase and/or .beta.-amylase thereto. By the use of such three-dimensional, cross-linked high molecular weight substances, glucoamylase and .beta.-amylase can be isolated and purified extremely efficiently.

Abstract: A culturing method and system are disclosed in which a cell culture broth in withdrawn from a culturing apparatus to a filteration apparatus. The withdrawn broth in brought into contact with a membrane for precision filteration. Then a non-toxic gas is brown into the broth, while filtering the broth to separate the cells and breaking foams occurring on the surface of the broth due to gas bubbling. The separated cells are returned to the vessel and the filterate is withdrawn from the filteration apparatus.The method and system have such advantages that a perfusion culture of a long period of time can be realized because the clogging of the filteration membrane is effectively prevented.

Abstract: An apparatus for culturing animal cells, includes a diagnosis system to make it possible to diagnose by measuring sizes of the cells by means of image processing while a system of culturing the animal cells is cut off from the outside of the apparatus system, a control system to control the culture conditions of a culturing cistern on the basis of a result of the diagnosis, and a culture medium regenerating system to remove waste contents in a culture medium. In the apparatus of this invention, the culture system and diagnosis system are cut off from the outside of the apparatus system. Therefore, no infection of miscellaneous microorganism, etc., occurs, nor does consumption of valuable cells and products occur. Further, since it is possible to easily obtain data of the proportions of division potential-possessing cells and living cells, the environmental factors to the culturing cistern can be easily and instantly controlled based on the data, and therefore, the culturing efficiency can be enhanced.

Abstract: A centrifugal separation method for living cells is disclosed in which a cell containing solution is charged in a rotor chamber confined by a steam pressure-resistant wall. A rotor having septa is disposed in the rotor chamber. The septa are rotatable in the same direction at the same angular velocity as the rotor while at least part thereof is immersed in a solution in the rotor when cells are to be centrifuged from the cell-containing solution. After the cells are separated and adhered to the inner surface of the rotor, a supernatant in the rotor is discharged from the rotor. Then a fresh cultivating medium is introduced into the rotor, and the septa and the rotor are rotated at mutually different angular velocities while at least part of the septa is immersed in the fresh medium in the rotor. As a result, the cells are again suspended in the fresh medium.

Abstract: An immobilized enzyme having high activity and stability is obtained by immobilizing an enzyme on a water-insoluble amino group-containing carrier by use of a polyfunctional cross-linking agent such as glutaraldehyde in the presence of a phenolic carboxylic acid having one or more hydroxyl groups such as tannic acid. In addition to the phenolic carboxylic acid, a basic polysaccharide such as chitosan may also be present. The amino group-containing carrier may be aminated silica gel, aminated porous glass, aminated zeolite, or water insoluble crosslinked chitosen.