Patents by Inventor Ryotaro Kotani

Ryotaro Kotani has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Process for producing tissue plasminogen activator
    Patent number: 4935368
    Abstract: This invention discloses a process for producing tissue plasminogen activator. The process comprises cultivating Escherichia coli, yeast, or mammalian cells into which the vector having the gene for human tissue plasminogen activator (tPA) has been introduced by the recombinant DNA technology. In the cultivation or purification process, a specific antiplasmin agent, which is one of the protease inhibitors, is added to the medium and/or the buffer solutions used for purification, so as to prevent the conversion of single-chain form tPA into double-chain form tPA by a protease which is present in the medium.
    Type: Grant
    Filed: June 27, 1986
    Date of Patent: June 19, 1990
    Assignees: Toyo Boseki Kabushiki Kaisha, Daiichi Seiyaku Co., Ltd.
    Inventors: Ryotaro Kotani, Tsuneo Unuma, Shigeki Otawara, Setsuo Kobayashi, Tadao Suzuki
  • Stable enzyme composition
    Patent number: 4451569
    Abstract: There is provided a stable enzyme composition comprising glutathione peroxidase and at least one stabilizer compound selected from the group consisting of pentoses, hexoses, penthahydric sugar alcohols, hexahydric sugar alcohols and disaccharides.
    Type: Grant
    Filed: July 1, 1982
    Date of Patent: May 29, 1984
    Assignee: Toyo Boseki Kabushiki Kaisha
    Inventors: Setsuo Kobayashi, Kazuo Beppu, Hiroshi Shimizu, Satoshi Ogawa, Ryotaro Kotani