Abstract: A method for editing a plant genome includes coating a microparticle with at least one type of nucleic acid and/or at least one type of protein, introducing a deletion, insertion, or substitution into a target site in the genome of a plant by bombarding a shoot apex of the plant with the coated microparticle using a gene gun, growing the shoot apex bombarded with the coated microparticle to obtain a plant body, and selecting a genome-edited plant body from the plant body. The shoot apex of the plant is selected from the group consisting of a shoot apex of an embryo of a fully mature seed, a shoot apex of a young bud of a tuber, and a shoot apex of a terminal bud or a lateral bud.

Abstract: A method to transforming a plant includes coating a microparticle having a diameter of 0.3 to 0.9 ?m with at least one type of nucleic acid, bombarding a shoot apex of the plant with the coated microparticle using a gene gun, growing the shoot apex bombarded with the coated microparticle to obtain a plant body, and selecting a transformed plant body from the plant body. The shoot apex is selected from the group consisting of a shoot apex of an embryo of a fully mature seed, a shoot apex of a young bud of a tuber, and a shoot apex of a terminal bud or a lateral bud.

Abstract: This invention provides a method of plant transformation via Agrobacterium comprising inoculating and infecting a plant with an Agrobacterium carrying a foreign-gene-containing vector under application of a plant growth-inhibiting hormone. This invention also provides a method of plant transformation via Agrobacterium comprising transforming a plant with an Agrobacterium carrying a vector comprising a T-DNA region in which a foreign gene is contained but no selectable marker gene is contained.

Abstract: A method to transforming a plant includes coating a microparticle having a diameter of 0.3 to 0.9 ?m with at least one type of nucleic acid, bombarding a shoot apex of the plant with the coated microparticle using a gene gun, growing the shoot apex bombarded with the coated microparticle to obtain a plant body, and selecting a transformed plant body from the plant body. The shoot apex is selected from the group consisting of a shoot apex of an embryo of a fully mature seed, a shoot apex of a young bud of a tuber, and a shoot apex of a terminal bud or a lateral bud.

Abstract: A method for editing a plant genome includes coating a microparticle with at least one type of nucleic acid and/or at least one type of protein, introducing a deletion, insertion, or substitution into a target site in the genome of a plant by bombarding a shoot apex of the plant with the coated microparticle using a gene gun, growing the shoot apex bombarded with the coated microparticle to obtain a plant body, and selecting a genome-edited plant body from the plant body. The shoot apex of the plant is selected from the group consisting of a shoot apex of an embryo of a fully mature seed, a shoot apex of a young bud of a tuber, and a shoot apex of a terminal bud or a lateral bud.

Abstract: It is an object solved by the present invention to provide an Agrobacterium with increased gene targeting efficiency. The present invention provides an Agrobacterium having a T-DNA nuclear translocation ability equivalent to that of a wild-type Agrobacterium and also having a T-DNA chromosomal insertion ability that is lost or reduced in comparison to a wild-type Agrobacterium.

Abstract: This invention provides a method of plant transformation via Agrobacterium comprising inoculating and infecting a plant with an Agrobacterium carrying a foreign-gene-containing vector under application of a plant growth-inhibiting hormone. This invention also provides a method of plant transformation via Agrobacterium comprising transforming a plant with an Agrobacterium carrying a vector comprising a T-DNA region in which a foreign gene is contained but no selectable marker gene is contained.

Abstract: This invention relates to a plant having high resistance to environmental stress and high seed productivity, and, more particularly, a transformed plant that is genetically modified to overexpress a polyadenylate-binding protein (PABN) gene and has enhanced environmental stress resistance and enhanced seed productivity.